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Molecular analysis of an odorant-binding protein gene in two sympatric species of Lutzomyia longipalpis s.l.

Dias AK, Bauzer LG, Santos Dias DB, Peixoto AA - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: DNA samples from two Brazilian sympatric species that differ in pheromone and courtship song production were used to analyse molecular polymorphisms in an odorant-binding protein (obp29) gene.OBPs are proteins related to olfaction and are involved in activities fundamental to survival, such as foraging, mating and choice of oviposition site.In this study, the marker obp29 was found to be highly polymorphic in Lu. longipalpis s.l. , with no fixed differences observed between the two species.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, Fiocruz, Brasil, Rio de JaneiroRJ, Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.

ABSTRACT
Lutzomyia longipalpis s.l. is the main vector of American visceral leishmaniasis (AVL) and occurs as a species complex. DNA samples from two Brazilian sympatric species that differ in pheromone and courtship song production were used to analyse molecular polymorphisms in an odorant-binding protein (obp29) gene. OBPs are proteins related to olfaction and are involved in activities fundamental to survival, such as foraging, mating and choice of oviposition site. In this study, the marker obp29 was found to be highly polymorphic in Lu. longipalpis s.l. , with no fixed differences observed between the two species. A pairwise fixation index test indicated a moderate level of genetic differentiation between the samples analysed.

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Related in: MedlinePlus

Alignment of the variable sites in the Lutzomyia longipalpis s.l.odorant-binding protein 29  haplotypic sequences. Dots indicatenucleotides shared with the first sequence. Positions of the polymorphic sites areindicated on the top of the figure. Non synonymous sites are shown ingray.
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f01: Alignment of the variable sites in the Lutzomyia longipalpis s.l.odorant-binding protein 29 haplotypic sequences. Dots indicatenucleotides shared with the first sequence. Positions of the polymorphic sites areindicated on the top of the figure. Non synonymous sites are shown ingray.

Mentions: A 685-bp opb29 fragment encompassing the coding region and part of the 3’UTR was obtained. A total of 18 consensus sequences were analysed from the Sobral 1S and 2Spopulations. Of the 685 analysed nucleotides sites, 93 (13.6%) were variable and thenucleotide diversity estimation was similar for the two species analysed (π = 0.035 forSobral 1S and π = 0.0331 for Sobral 2S). Similarly, the parameter θ was found to beessentially the same for the two species (0.0310 and 0.0309 for Sobral 1S and Sobral 2S,respectively). Figure shows an alignment of all thepolymorphic sites observed in the analysed opb29 fragment. Twenty threepolymorphic sites were exclusive to Sobral 1S, 24 were exclusive to Sobral 2S and 54 wereshared between the species; non-synonymous mutations were found at 12 sites (6 exclusive toS1S, 3 exclusive to S2S and 3 shared). A moderate and significant genetic differentiation(Fst = 0.1098; p < 0.001; 1,000 permutations) was computed between the two species. Thegenetic divergence and polymorphism data were used to test departures from neutrality, withboth Tajima’s D statistics ( Tajima 1989 ) and theHKA test ( Hudson et al. 1987 ) indicating nodeparture from neutrality.


Molecular analysis of an odorant-binding protein gene in two sympatric species of Lutzomyia longipalpis s.l.

Dias AK, Bauzer LG, Santos Dias DB, Peixoto AA - Mem. Inst. Oswaldo Cruz (2013)

Alignment of the variable sites in the Lutzomyia longipalpis s.l.odorant-binding protein 29  haplotypic sequences. Dots indicatenucleotides shared with the first sequence. Positions of the polymorphic sites areindicated on the top of the figure. Non synonymous sites are shown ingray.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109184&req=5

f01: Alignment of the variable sites in the Lutzomyia longipalpis s.l.odorant-binding protein 29 haplotypic sequences. Dots indicatenucleotides shared with the first sequence. Positions of the polymorphic sites areindicated on the top of the figure. Non synonymous sites are shown ingray.
Mentions: A 685-bp opb29 fragment encompassing the coding region and part of the 3’UTR was obtained. A total of 18 consensus sequences were analysed from the Sobral 1S and 2Spopulations. Of the 685 analysed nucleotides sites, 93 (13.6%) were variable and thenucleotide diversity estimation was similar for the two species analysed (π = 0.035 forSobral 1S and π = 0.0331 for Sobral 2S). Similarly, the parameter θ was found to beessentially the same for the two species (0.0310 and 0.0309 for Sobral 1S and Sobral 2S,respectively). Figure shows an alignment of all thepolymorphic sites observed in the analysed opb29 fragment. Twenty threepolymorphic sites were exclusive to Sobral 1S, 24 were exclusive to Sobral 2S and 54 wereshared between the species; non-synonymous mutations were found at 12 sites (6 exclusive toS1S, 3 exclusive to S2S and 3 shared). A moderate and significant genetic differentiation(Fst = 0.1098; p < 0.001; 1,000 permutations) was computed between the two species. Thegenetic divergence and polymorphism data were used to test departures from neutrality, withboth Tajima’s D statistics ( Tajima 1989 ) and theHKA test ( Hudson et al. 1987 ) indicating nodeparture from neutrality.

Bottom Line: DNA samples from two Brazilian sympatric species that differ in pheromone and courtship song production were used to analyse molecular polymorphisms in an odorant-binding protein (obp29) gene.OBPs are proteins related to olfaction and are involved in activities fundamental to survival, such as foraging, mating and choice of oviposition site.In this study, the marker obp29 was found to be highly polymorphic in Lu. longipalpis s.l. , with no fixed differences observed between the two species.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, Fiocruz, Brasil, Rio de JaneiroRJ, Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.

ABSTRACT
Lutzomyia longipalpis s.l. is the main vector of American visceral leishmaniasis (AVL) and occurs as a species complex. DNA samples from two Brazilian sympatric species that differ in pheromone and courtship song production were used to analyse molecular polymorphisms in an odorant-binding protein (obp29) gene. OBPs are proteins related to olfaction and are involved in activities fundamental to survival, such as foraging, mating and choice of oviposition site. In this study, the marker obp29 was found to be highly polymorphic in Lu. longipalpis s.l. , with no fixed differences observed between the two species. A pairwise fixation index test indicated a moderate level of genetic differentiation between the samples analysed.

Show MeSH
Related in: MedlinePlus