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Male accessory gland substances from Aedes albopictus affect the locomotor activity of Aedes aegypti females.

Lima-Camara TN, Codeço CT, Honório NA, Bruno RV, Peixoto AA, Lounibos LP - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: These two diurnal mosquitoes are frequently found coexisting in similar habitats, enabling interactions between adults, such as cross-mating.Virgin Ae. aegypti females injected with conspecific and heterospecific MAGs showed a general decrease in locomotor activity compared to controls and were refractory to mating with conspecific males.The reduction in diurnal locomotor activity induced by injections of conspecific or heterospecific MAGs is consistent with regulation of female reproductive activities by male substances, which are capable of sterilising female Ae. aegypti through satyrisation by Ae. albopictus.

View Article: PubMed Central - PubMed

Affiliation: Programa de Computação Científica, Programa de Computação Científica.

ABSTRACT
Dengue is one of the world's most important mosquito-borne diseases and is usually transmitted by one of two vector species: Aedes aegypti or Aedes albopictus . These two diurnal mosquitoes are frequently found coexisting in similar habitats, enabling interactions between adults, such as cross-mating. The objective of this study was to assess cross-mating between Ae. aegypti females and Ae. albopictus males under artificial conditions and evaluate the locomotor activity of Ae. aegypti virgin females injected with male accessory gland (MAG) homogenates to infer the physiological and behavioural responses to interspecific mating. After seven days of exposure, 3.3-16% of Ae. aegypti females mated with Ae. albopictus males. Virgin Ae. aegypti females injected with conspecific and heterospecific MAGs showed a general decrease in locomotor activity compared to controls and were refractory to mating with conspecific males. The reduction in diurnal locomotor activity induced by injections of conspecific or heterospecific MAGs is consistent with regulation of female reproductive activities by male substances, which are capable of sterilising female Ae. aegypti through satyrisation by Ae. albopictus.

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: boxplots of means of Aedes aegypti females injected withsaline (n = 64), accessory gland (AG) of Ae. aegypti males(Con) (n = 56) and AG of Aedes albopictus  males (Hetero) (n =70). A: total activity (diurnal + nocturnal); B: diurnal activity; C: nocturnalactivity; D: lights-on activity. Different letters identify groups that werestatistically different (p < 0.05) according to the Tukey test.
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f03: : boxplots of means of Aedes aegypti females injected withsaline (n = 64), accessory gland (AG) of Ae. aegypti males(Con) (n = 56) and AG of Aedes albopictus males (Hetero) (n =70). A: total activity (diurnal + nocturnal); B: diurnal activity; C: nocturnalactivity; D: lights-on activity. Different letters identify groups that werestatistically different (p < 0.05) according to the Tukey test.

Mentions: The total activity of the three tested groups showed a significant effect of status(injected with saline vs. injected with AG) [F = 10.536; degree of freedom (df) = 2; p< 0.001], but no significant block effects (F = 0.292; df = 1; p = 0.590) orinteraction between block and status (F = 1.699; df = 2; p = 0.186). Tukey test ( Fig. 3 ) showed a significantly reduced activity ofAe. aegypti virgin females injected with conspecific MAG as comparedto the control group (p < 0.001). The total locomotor activity of females injectedwith Ae. albopictus MAG was lower than the control group as well (Table I ), but the difference was notstatistically significant (p = 0.156), remaining higher than those injected withconspecific MAG ( Fig. 3 ). When the locomotoractivity is stratified according to time of day, it becomes evident that only thediurnal behaviour is affected by MAG ( Fig. 3 ).Considering the lights-on peak, that is, the transition between dark and light providesthe sharpest discrimination among groups with both MAG-injected groups showingsignificantly less activity than the control group, with conspecific MAG inducingsimilar decrease of heterospecific MAG.


Male accessory gland substances from Aedes albopictus affect the locomotor activity of Aedes aegypti females.

Lima-Camara TN, Codeço CT, Honório NA, Bruno RV, Peixoto AA, Lounibos LP - Mem. Inst. Oswaldo Cruz (2013)

: boxplots of means of Aedes aegypti females injected withsaline (n = 64), accessory gland (AG) of Ae. aegypti males(Con) (n = 56) and AG of Aedes albopictus  males (Hetero) (n =70). A: total activity (diurnal + nocturnal); B: diurnal activity; C: nocturnalactivity; D: lights-on activity. Different letters identify groups that werestatistically different (p < 0.05) according to the Tukey test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109176&req=5

f03: : boxplots of means of Aedes aegypti females injected withsaline (n = 64), accessory gland (AG) of Ae. aegypti males(Con) (n = 56) and AG of Aedes albopictus males (Hetero) (n =70). A: total activity (diurnal + nocturnal); B: diurnal activity; C: nocturnalactivity; D: lights-on activity. Different letters identify groups that werestatistically different (p < 0.05) according to the Tukey test.
Mentions: The total activity of the three tested groups showed a significant effect of status(injected with saline vs. injected with AG) [F = 10.536; degree of freedom (df) = 2; p< 0.001], but no significant block effects (F = 0.292; df = 1; p = 0.590) orinteraction between block and status (F = 1.699; df = 2; p = 0.186). Tukey test ( Fig. 3 ) showed a significantly reduced activity ofAe. aegypti virgin females injected with conspecific MAG as comparedto the control group (p < 0.001). The total locomotor activity of females injectedwith Ae. albopictus MAG was lower than the control group as well (Table I ), but the difference was notstatistically significant (p = 0.156), remaining higher than those injected withconspecific MAG ( Fig. 3 ). When the locomotoractivity is stratified according to time of day, it becomes evident that only thediurnal behaviour is affected by MAG ( Fig. 3 ).Considering the lights-on peak, that is, the transition between dark and light providesthe sharpest discrimination among groups with both MAG-injected groups showingsignificantly less activity than the control group, with conspecific MAG inducingsimilar decrease of heterospecific MAG.

Bottom Line: These two diurnal mosquitoes are frequently found coexisting in similar habitats, enabling interactions between adults, such as cross-mating.Virgin Ae. aegypti females injected with conspecific and heterospecific MAGs showed a general decrease in locomotor activity compared to controls and were refractory to mating with conspecific males.The reduction in diurnal locomotor activity induced by injections of conspecific or heterospecific MAGs is consistent with regulation of female reproductive activities by male substances, which are capable of sterilising female Ae. aegypti through satyrisation by Ae. albopictus.

View Article: PubMed Central - PubMed

Affiliation: Programa de Computação Científica, Programa de Computação Científica.

ABSTRACT
Dengue is one of the world's most important mosquito-borne diseases and is usually transmitted by one of two vector species: Aedes aegypti or Aedes albopictus . These two diurnal mosquitoes are frequently found coexisting in similar habitats, enabling interactions between adults, such as cross-mating. The objective of this study was to assess cross-mating between Ae. aegypti females and Ae. albopictus males under artificial conditions and evaluate the locomotor activity of Ae. aegypti virgin females injected with male accessory gland (MAG) homogenates to infer the physiological and behavioural responses to interspecific mating. After seven days of exposure, 3.3-16% of Ae. aegypti females mated with Ae. albopictus males. Virgin Ae. aegypti females injected with conspecific and heterospecific MAGs showed a general decrease in locomotor activity compared to controls and were refractory to mating with conspecific males. The reduction in diurnal locomotor activity induced by injections of conspecific or heterospecific MAGs is consistent with regulation of female reproductive activities by male substances, which are capable of sterilising female Ae. aegypti through satyrisation by Ae. albopictus.

Show MeSH
Related in: MedlinePlus