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Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector.

Seixas G, Salgueiro P, Silva AC, Campos M, Spenassatto C, Reyes-Lugo M, Novo MT, Ribolla PE, Silva Pinto JP, Sousa CA - Mem. Inst. Oswaldo Cruz (2013)

Bottom Line: We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C).These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance.To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes.

View Article: PubMed Central - PubMed

Affiliation: Unidade de Ensino e Investigação-Parasitologia Médica, Unidade de Ensino e Investigação-Parasitologia Médica.

ABSTRACT
The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance (kdr) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.

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: map showing the geographic collection sites.
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f01: : map showing the geographic collection sites.

Mentions: Mosquito samples and DNA extraction - Samples of Ae. aegypti were collected on Madeira Island and from two populations of Brazil andVenezuela ( Figure , Table I ). Larvae and eggs were collected and reared to adults in aninsectary under standard conditions. Adults were kept in dry, individual tubes withsilica gel until molecular analyses.


Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector.

Seixas G, Salgueiro P, Silva AC, Campos M, Spenassatto C, Reyes-Lugo M, Novo MT, Ribolla PE, Silva Pinto JP, Sousa CA - Mem. Inst. Oswaldo Cruz (2013)

: map showing the geographic collection sites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109174&req=5

f01: : map showing the geographic collection sites.
Mentions: Mosquito samples and DNA extraction - Samples of Ae. aegypti were collected on Madeira Island and from two populations of Brazil andVenezuela ( Figure , Table I ). Larvae and eggs were collected and reared to adults in aninsectary under standard conditions. Adults were kept in dry, individual tubes withsilica gel until molecular analyses.

Bottom Line: We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C).These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance.To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes.

View Article: PubMed Central - PubMed

Affiliation: Unidade de Ensino e Investigação-Parasitologia Médica, Unidade de Ensino e Investigação-Parasitologia Médica.

ABSTRACT
The increasing population of Aedes aegypti mosquitoes on Madeira Island (Portugal) resulted in the first autochthonous dengue outbreak, which occurred in October 2012. Our study establishes the first genetic evaluation based on the mitochondrial DNA (mtDNA) genes [cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 4 (ND4)] and knockdown resistance (kdr) mutations exploring the colonisation history and the genetic diversity of this insular vector population. We included mosquito populations from Brazil and Venezuela in the analysis as putative geographic sources. The Ae. aegypti population from Madeira showed extremely low mtDNA genetic variability, with a single haplotype for COI and ND4. We also detected the presence of two important kdr mutations and the quasi-fixation of one of these mutations (F1534C). These results are consistent with a unique recent founder event that occurred on the island of Ae. aegypti mosquitoes that carry kdr mutations associated with insecticide resistance. Finally, we also report the presence of the F1534C kdr mutation in the Brazil and Venezuela populations. To our knowledge, this is the first time this mutation has been found in South American Ae. aegypti mosquitoes. Given the present risk of Ae. aegypti re-invading continental Europe from Madeira and the recent dengue outbreaks on the island, this information is important to plan surveillance and control measures.

Show MeSH
Related in: MedlinePlus