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Novel functional changes during podocyte differentiation: increase of oxidative resistance and H-ferritin expression.

Bányai E, Balogh E, Fagyas M, Arosio P, Hendrik Z, Király G, Nagy G, Tánczos B, Pócsi I, Balla G, Balla J, Bánfalvi G, Jeney V - Oxid Med Cell Longev (2014)

Bottom Line: We observed that differentiated podocytes were highly resistant to oxidants such as H2O2 and heme when applied separately or in combination, whereas undifferentiated cells were prone to such challenges.Elevated oxidative resistance of differentiated podocytes was associated with increased activities of antioxidant enzymes and H-ferritin expression.Immunohistochemical analysis of normal human kidney specimens revealed that podocytes highly express H-ferritin in vivo as well.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, University of Debrecen, Debrecen 4032, Hungary.

ABSTRACT
Podocytes are highly specialized, arborized epithelial cells covering the outer surface of the glomerular tuft in the kidney. Terminally differentiated podocytes are unable to go through cell division and hereby they are lacking a key property for regeneration after a toxic injury. Podocytes are long-lived cells but, to date, little is known about the mechanisms that support their stress resistance. Our aim was to investigate whether the well-known morphological changes during podocyte differentiation are accompanied by changes in oxidative resistance in a manner that could support their long-term survival. We used a conditionally immortalized human podocyte cell line to study the morphological and functional changes during differentiation. We followed the differentiation process for 14 days by time-lapse microscopy. During this period nondifferentiated podocytes gradually transformed into large, nonproliferating, frequently multinucleated cells, with enlarged nuclei and opened chromatin structure. We observed that differentiated podocytes were highly resistant to oxidants such as H2O2 and heme when applied separately or in combination, whereas undifferentiated cells were prone to such challenges. Elevated oxidative resistance of differentiated podocytes was associated with increased activities of antioxidant enzymes and H-ferritin expression. Immunohistochemical analysis of normal human kidney specimens revealed that podocytes highly express H-ferritin in vivo as well.

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Schematic illustration of phenotype alterations during podocyte differentiation. Morphological changes characterized by alteration in cell body, nucleus size, and chromatin condensation accompanied by functional changes such as increase in oxidative resistance and FtH expression during podocyte differentiation are shown.
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fig8: Schematic illustration of phenotype alterations during podocyte differentiation. Morphological changes characterized by alteration in cell body, nucleus size, and chromatin condensation accompanied by functional changes such as increase in oxidative resistance and FtH expression during podocyte differentiation are shown.

Mentions: This study provides the first evidence that, during embryonic differentiation, podocytes go through fundamental morphological and functional changes assisting them to intensify defence mechanisms against oxidative stress damage and increase their resistance essential for long life duration (Figure 8). Antioxidant enzymes and FtH might contribute to the protection of human glomerular podocytes against oxidative injuries and highlight the so far unrevealed but certainly central importance of this protective system in glomerular diseases.


Novel functional changes during podocyte differentiation: increase of oxidative resistance and H-ferritin expression.

Bányai E, Balogh E, Fagyas M, Arosio P, Hendrik Z, Király G, Nagy G, Tánczos B, Pócsi I, Balla G, Balla J, Bánfalvi G, Jeney V - Oxid Med Cell Longev (2014)

Schematic illustration of phenotype alterations during podocyte differentiation. Morphological changes characterized by alteration in cell body, nucleus size, and chromatin condensation accompanied by functional changes such as increase in oxidative resistance and FtH expression during podocyte differentiation are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109136&req=5

fig8: Schematic illustration of phenotype alterations during podocyte differentiation. Morphological changes characterized by alteration in cell body, nucleus size, and chromatin condensation accompanied by functional changes such as increase in oxidative resistance and FtH expression during podocyte differentiation are shown.
Mentions: This study provides the first evidence that, during embryonic differentiation, podocytes go through fundamental morphological and functional changes assisting them to intensify defence mechanisms against oxidative stress damage and increase their resistance essential for long life duration (Figure 8). Antioxidant enzymes and FtH might contribute to the protection of human glomerular podocytes against oxidative injuries and highlight the so far unrevealed but certainly central importance of this protective system in glomerular diseases.

Bottom Line: We observed that differentiated podocytes were highly resistant to oxidants such as H2O2 and heme when applied separately or in combination, whereas undifferentiated cells were prone to such challenges.Elevated oxidative resistance of differentiated podocytes was associated with increased activities of antioxidant enzymes and H-ferritin expression.Immunohistochemical analysis of normal human kidney specimens revealed that podocytes highly express H-ferritin in vivo as well.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, University of Debrecen, Debrecen 4032, Hungary.

ABSTRACT
Podocytes are highly specialized, arborized epithelial cells covering the outer surface of the glomerular tuft in the kidney. Terminally differentiated podocytes are unable to go through cell division and hereby they are lacking a key property for regeneration after a toxic injury. Podocytes are long-lived cells but, to date, little is known about the mechanisms that support their stress resistance. Our aim was to investigate whether the well-known morphological changes during podocyte differentiation are accompanied by changes in oxidative resistance in a manner that could support their long-term survival. We used a conditionally immortalized human podocyte cell line to study the morphological and functional changes during differentiation. We followed the differentiation process for 14 days by time-lapse microscopy. During this period nondifferentiated podocytes gradually transformed into large, nonproliferating, frequently multinucleated cells, with enlarged nuclei and opened chromatin structure. We observed that differentiated podocytes were highly resistant to oxidants such as H2O2 and heme when applied separately or in combination, whereas undifferentiated cells were prone to such challenges. Elevated oxidative resistance of differentiated podocytes was associated with increased activities of antioxidant enzymes and H-ferritin expression. Immunohistochemical analysis of normal human kidney specimens revealed that podocytes highly express H-ferritin in vivo as well.

Show MeSH
Related in: MedlinePlus