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An emerging translational model to screen potential medicinal plants for nephrolithiasis, an independent risk factor for chronic kidney disease.

Wu SY, Shen JL, Man KM, Lee YJ, Chen HY, Chen YH, Tsai KS, Tsai FJ, Lin WY, Chen WC - Evid Based Complement Alternat Med (2014)

Bottom Line: Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies.Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects.These useful medicinal plants can be further studied using other animal or human models to verify their effects.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Graduate Institute of Chinese Medicine, Graduate Institute of Integrated Medicine, College of Chinese Medicine, Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
Pharmacological therapy for urolithiasis using medicinal plants has been increasingly adopted for the prevention of its recurrence. A Drosophila melanogaster model developed for translational research of urolithiasis was applied to evaluate agents with potential antilithic effects and calcium oxalate (CaOx) formation. Potential antilithic herbs were prepared in a mixture of food in a diluted concentration of 5,000 from the original extract with 0.5% ethylene glycol (EG) as the lithogenic agent. The control group was fed with food only. After 3 weeks, flies (n ≥ 150 for each group) were killed using CO2 narcotization, and the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination of the crystals. The crystal formation rate in the EG group was 100.0%. In the study, 16 tested herbal drugs reached the crystal formation rate of 0.0%, including Salviae miltiorrhizae, Paeonia lactiflora, and Carthami flos. Scutellaria baicalensis enhanced CaOx crystal formation. Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies. Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects. These useful medicinal plants can be further studied using other animal or human models to verify their effects.

No MeSH data available.


Related in: MedlinePlus

EG-induced CaOx crystal deposition in the Malpighian tubules. The images show representative polarized microscopy for the (a) control flies and (b) the flies with 0.5% EG-induced crystal formation in Malpighian tubules.
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fig1: EG-induced CaOx crystal deposition in the Malpighian tubules. The images show representative polarized microscopy for the (a) control flies and (b) the flies with 0.5% EG-induced crystal formation in Malpighian tubules.

Mentions: The crystal formation rate in EG and control groups was 100.0% and 10.2%, respectively. Positive CaOx crystal formation can be seen in the Malpighian tubules of flies (Figure 1). In the first study, 16 tested herbal drugs reached the crystal formation rate of 0% (Table 1), namely, Salviae miltiorrhizae (number 11, 丹參), Paeonia lactiflora (number 19, 白芍藥), Carthami flos (number 21, 紅花), Corydalis yanhusuo (number 29, 延胡索), Imperata cylindrica (number 35, 白茅根), Prunus armeniaca (number 42, 杏仁), Eclipta prostrata (number 43, 旱蓮草), Artemisia argyi (number 46, 艾葉), Plantago asiatica (number 48 and its seed number 50, 車前子), Lonicera japonica (number 49, 忍冬藤), Polygoni cuspidati (number 52, 虎杖), Astragalus membranaceus (number 67, 黃耆), Wolfiporia cocos (number 70, 茯苓), Scutellaria baicalensis (number 76, 黃芩), and Angelicae sinensis (number 77, 當歸).


An emerging translational model to screen potential medicinal plants for nephrolithiasis, an independent risk factor for chronic kidney disease.

Wu SY, Shen JL, Man KM, Lee YJ, Chen HY, Chen YH, Tsai KS, Tsai FJ, Lin WY, Chen WC - Evid Based Complement Alternat Med (2014)

EG-induced CaOx crystal deposition in the Malpighian tubules. The images show representative polarized microscopy for the (a) control flies and (b) the flies with 0.5% EG-induced crystal formation in Malpighian tubules.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109113&req=5

fig1: EG-induced CaOx crystal deposition in the Malpighian tubules. The images show representative polarized microscopy for the (a) control flies and (b) the flies with 0.5% EG-induced crystal formation in Malpighian tubules.
Mentions: The crystal formation rate in EG and control groups was 100.0% and 10.2%, respectively. Positive CaOx crystal formation can be seen in the Malpighian tubules of flies (Figure 1). In the first study, 16 tested herbal drugs reached the crystal formation rate of 0% (Table 1), namely, Salviae miltiorrhizae (number 11, 丹參), Paeonia lactiflora (number 19, 白芍藥), Carthami flos (number 21, 紅花), Corydalis yanhusuo (number 29, 延胡索), Imperata cylindrica (number 35, 白茅根), Prunus armeniaca (number 42, 杏仁), Eclipta prostrata (number 43, 旱蓮草), Artemisia argyi (number 46, 艾葉), Plantago asiatica (number 48 and its seed number 50, 車前子), Lonicera japonica (number 49, 忍冬藤), Polygoni cuspidati (number 52, 虎杖), Astragalus membranaceus (number 67, 黃耆), Wolfiporia cocos (number 70, 茯苓), Scutellaria baicalensis (number 76, 黃芩), and Angelicae sinensis (number 77, 當歸).

Bottom Line: Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies.Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects.These useful medicinal plants can be further studied using other animal or human models to verify their effects.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Graduate Institute of Chinese Medicine, Graduate Institute of Integrated Medicine, College of Chinese Medicine, Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
Pharmacological therapy for urolithiasis using medicinal plants has been increasingly adopted for the prevention of its recurrence. A Drosophila melanogaster model developed for translational research of urolithiasis was applied to evaluate agents with potential antilithic effects and calcium oxalate (CaOx) formation. Potential antilithic herbs were prepared in a mixture of food in a diluted concentration of 5,000 from the original extract with 0.5% ethylene glycol (EG) as the lithogenic agent. The control group was fed with food only. After 3 weeks, flies (n ≥ 150 for each group) were killed using CO2 narcotization, and the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination of the crystals. The crystal formation rate in the EG group was 100.0%. In the study, 16 tested herbal drugs reached the crystal formation rate of 0.0%, including Salviae miltiorrhizae, Paeonia lactiflora, and Carthami flos. Scutellaria baicalensis enhanced CaOx crystal formation. Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies. Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects. These useful medicinal plants can be further studied using other animal or human models to verify their effects.

No MeSH data available.


Related in: MedlinePlus