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Matrix metalloproteinases 2 and 9 immunoexpression in prostate carcinoma at the positive margin of radical prostatectomy specimens.

Oguić R, Mozetič V, Cini Tešar E, Fučkar Čupić D, Mustać E, Dorđević G - Patholog Res Int (2014)

Bottom Line: Tissue microarrays of 120 archival prostate carcinoma samples were immunohistochemically evaluated for MMP-2 and MMP-9 expression and compared with clinicopathological parameters.In the group of patients with negative margins, MMP-9 expression above the cut-off value was significantly associated with recurrence (P = 0.0065).Multivariate analysis indicated that MMP-9 is a good predictor of biochemical recurrence (odds ratio = 10.29; P = 0.0052).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Clinical Hospital Centre Rijeka, Krešimirova 42, 51000 Rijeka, Croatia.

ABSTRACT
The aim of this study was to evaluate the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) in prostate cancer in the main tumor mass and tumor cells at the positive margin as well as the influence of these biomarkers on the biochemical recurrence of the disease in prostatectomy patients. Tissue microarrays of 120 archival prostate carcinoma samples were immunohistochemically evaluated for MMP-2 and MMP-9 expression and compared with clinicopathological parameters. Tumors with positive surgical margins showed significantly higher overall expression of MMP-9 versus tumors with negative resection margins (P = 0.0121). MMP-9 expression was significantly elevated in tumors from patients who had biochemical recurrence (P = 0.0207). In the group of patients with negative margins, MMP-9 expression above the cut-off value was significantly associated with recurrence (P = 0.0065). Multivariate analysis indicated that MMP-9 is a good predictor of biochemical recurrence (odds ratio = 10.29; P = 0.0052). Expression of MMP-2 in tumor cells was significantly higher at the positive margins than in the main tumor mass (P = 0.0301). The present results highlight the potential value of MMP-2 and MMP-9 expression for predicting the behavior of prostate tumors after prostatectomy with both positive and negative surgical margins.

No MeSH data available.


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(a) Glands lined with hyperplastic epithelium and PIN (hematoxylin and eosin staining, 100x). (b) MMP-2 and MMP-9 immunohistochemistry indicates weak or negative expression in hyperplastic epithelium, except for the PIN, where the expression is strong (100x). (c) MMP-9 immunoexpression in moderately differentiated prostate cancer. Note the intense staining in the cytoplasm of tumor cells and the strongly positive reaction in the cytoplasm of stromal cells (200x). (d) MMP-2 immunoexpression in poorly differentiated prostate cancer. Note the strong cytoplasmic staining intensity (200x).
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fig3: (a) Glands lined with hyperplastic epithelium and PIN (hematoxylin and eosin staining, 100x). (b) MMP-2 and MMP-9 immunohistochemistry indicates weak or negative expression in hyperplastic epithelium, except for the PIN, where the expression is strong (100x). (c) MMP-9 immunoexpression in moderately differentiated prostate cancer. Note the intense staining in the cytoplasm of tumor cells and the strongly positive reaction in the cytoplasm of stromal cells (200x). (d) MMP-2 immunoexpression in poorly differentiated prostate cancer. Note the strong cytoplasmic staining intensity (200x).

Mentions: MMP-2 was expressed not only in the cytoplasm of tumor cells but also in the cytoplasm of prostatic stromal cells, endothelium, fibroblasts, smooth muscle cells, macrophages, and lymphocytes. The average histoscore of MMP-2 expression per tumor was 98.9160 (standard deviation (SD) 50.1750). MMP-2 was significantly more strongly expressed in tumor cells on the positive margins than in the main tumor mass, with an average of 109.0237 (SD 64.0751; P = 0.0301). In the stroma, the strongest MMP-2 expression occurred in the cytoplasm of inflammatory cells, especially macrophages (Figure 3).


Matrix metalloproteinases 2 and 9 immunoexpression in prostate carcinoma at the positive margin of radical prostatectomy specimens.

Oguić R, Mozetič V, Cini Tešar E, Fučkar Čupić D, Mustać E, Dorđević G - Patholog Res Int (2014)

(a) Glands lined with hyperplastic epithelium and PIN (hematoxylin and eosin staining, 100x). (b) MMP-2 and MMP-9 immunohistochemistry indicates weak or negative expression in hyperplastic epithelium, except for the PIN, where the expression is strong (100x). (c) MMP-9 immunoexpression in moderately differentiated prostate cancer. Note the intense staining in the cytoplasm of tumor cells and the strongly positive reaction in the cytoplasm of stromal cells (200x). (d) MMP-2 immunoexpression in poorly differentiated prostate cancer. Note the strong cytoplasmic staining intensity (200x).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4109076&req=5

fig3: (a) Glands lined with hyperplastic epithelium and PIN (hematoxylin and eosin staining, 100x). (b) MMP-2 and MMP-9 immunohistochemistry indicates weak or negative expression in hyperplastic epithelium, except for the PIN, where the expression is strong (100x). (c) MMP-9 immunoexpression in moderately differentiated prostate cancer. Note the intense staining in the cytoplasm of tumor cells and the strongly positive reaction in the cytoplasm of stromal cells (200x). (d) MMP-2 immunoexpression in poorly differentiated prostate cancer. Note the strong cytoplasmic staining intensity (200x).
Mentions: MMP-2 was expressed not only in the cytoplasm of tumor cells but also in the cytoplasm of prostatic stromal cells, endothelium, fibroblasts, smooth muscle cells, macrophages, and lymphocytes. The average histoscore of MMP-2 expression per tumor was 98.9160 (standard deviation (SD) 50.1750). MMP-2 was significantly more strongly expressed in tumor cells on the positive margins than in the main tumor mass, with an average of 109.0237 (SD 64.0751; P = 0.0301). In the stroma, the strongest MMP-2 expression occurred in the cytoplasm of inflammatory cells, especially macrophages (Figure 3).

Bottom Line: Tissue microarrays of 120 archival prostate carcinoma samples were immunohistochemically evaluated for MMP-2 and MMP-9 expression and compared with clinicopathological parameters.In the group of patients with negative margins, MMP-9 expression above the cut-off value was significantly associated with recurrence (P = 0.0065).Multivariate analysis indicated that MMP-9 is a good predictor of biochemical recurrence (odds ratio = 10.29; P = 0.0052).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Clinical Hospital Centre Rijeka, Krešimirova 42, 51000 Rijeka, Croatia.

ABSTRACT
The aim of this study was to evaluate the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) in prostate cancer in the main tumor mass and tumor cells at the positive margin as well as the influence of these biomarkers on the biochemical recurrence of the disease in prostatectomy patients. Tissue microarrays of 120 archival prostate carcinoma samples were immunohistochemically evaluated for MMP-2 and MMP-9 expression and compared with clinicopathological parameters. Tumors with positive surgical margins showed significantly higher overall expression of MMP-9 versus tumors with negative resection margins (P = 0.0121). MMP-9 expression was significantly elevated in tumors from patients who had biochemical recurrence (P = 0.0207). In the group of patients with negative margins, MMP-9 expression above the cut-off value was significantly associated with recurrence (P = 0.0065). Multivariate analysis indicated that MMP-9 is a good predictor of biochemical recurrence (odds ratio = 10.29; P = 0.0052). Expression of MMP-2 in tumor cells was significantly higher at the positive margins than in the main tumor mass (P = 0.0301). The present results highlight the potential value of MMP-2 and MMP-9 expression for predicting the behavior of prostate tumors after prostatectomy with both positive and negative surgical margins.

No MeSH data available.


Related in: MedlinePlus