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Cell transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer.

Lai WF, Jung HS - PLoS ONE (2014)

Bottom Line: This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery.In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity.Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT
Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

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The cytotoxicity of BPEA.The experiment was performed in (A and B) B16-F0, (C and D) HepG2 and (E and F) U87 cells both in the (A, C and E) absence and (B, D and F) presence of 10% FBS. PEI 25 kDa was used as a positive control to compare the relative cytotoxicity of BPEA.
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pone-0100258-g009: The cytotoxicity of BPEA.The experiment was performed in (A and B) B16-F0, (C and D) HepG2 and (E and F) U87 cells both in the (A, C and E) absence and (B, D and F) presence of 10% FBS. PEI 25 kDa was used as a positive control to compare the relative cytotoxicity of BPEA.

Mentions: The cytotoxicity of BPEA in B16-F0, HepG2 and U87 cells in the absence and presence of 10% FBS is shown in Fig. 9. The results suggested that, up to a concentration of 200 µg/ml, BPEA was basically non-toxic to all the cell lines tested. Comparatively, cells treated with PEI 25 kDa displayed a drastic drop in their viability with increasing concentrations of the polymer. The results of our hemolysis assay (Fig. 10) also showed that PEI 25 kDa induced approximately 60–80% hemolysis, which was approximately 2–4 times higher than the extent of hemoglobin release induced by BPEA.


Cell transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer.

Lai WF, Jung HS - PLoS ONE (2014)

The cytotoxicity of BPEA.The experiment was performed in (A and B) B16-F0, (C and D) HepG2 and (E and F) U87 cells both in the (A, C and E) absence and (B, D and F) presence of 10% FBS. PEI 25 kDa was used as a positive control to compare the relative cytotoxicity of BPEA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4067318&req=5

pone-0100258-g009: The cytotoxicity of BPEA.The experiment was performed in (A and B) B16-F0, (C and D) HepG2 and (E and F) U87 cells both in the (A, C and E) absence and (B, D and F) presence of 10% FBS. PEI 25 kDa was used as a positive control to compare the relative cytotoxicity of BPEA.
Mentions: The cytotoxicity of BPEA in B16-F0, HepG2 and U87 cells in the absence and presence of 10% FBS is shown in Fig. 9. The results suggested that, up to a concentration of 200 µg/ml, BPEA was basically non-toxic to all the cell lines tested. Comparatively, cells treated with PEI 25 kDa displayed a drastic drop in their viability with increasing concentrations of the polymer. The results of our hemolysis assay (Fig. 10) also showed that PEI 25 kDa induced approximately 60–80% hemolysis, which was approximately 2–4 times higher than the extent of hemoglobin release induced by BPEA.

Bottom Line: This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery.In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity.Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT
Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

Show MeSH
Related in: MedlinePlus