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Cell transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer.

Lai WF, Jung HS - PLoS ONE (2014)

Bottom Line: This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery.In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity.Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT
Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

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FT-IR spectra of (A) bPEI 12 kDa, (B) PEA and (C) BPEA.
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pone-0100258-g003: FT-IR spectra of (A) bPEI 12 kDa, (B) PEA and (C) BPEA.

Mentions: The successful generation of PEA and BPEA was further confirmed using FT-IR. In the spectrum of PEI (Fig. 3A), stretching vibration peaks of proton assigned to methyl and methylene could be found at 2920 cm−1 and 2822 cm−1. After cross-linking of PEI with propane-1,2,3-triol and due to the hydrogen bonding between N-H of PEI and C = O of propane-1,2,3-triol, an ester signal shifted to 1703 cm−1 in the spectrum of PEA (Fig. 3B). Finally, apart from the wide peak caused by the OH group at around 3200–3400 cm−1, O-C stretching vibration peaks of β-CD appeared at 1032, 1083 and1153 cm−1 in the spectrum of BPEA (Fig. 3C), suggesting successful incorporation of β-CD into PEA.


Cell transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer.

Lai WF, Jung HS - PLoS ONE (2014)

FT-IR spectra of (A) bPEI 12 kDa, (B) PEA and (C) BPEA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4067318&req=5

pone-0100258-g003: FT-IR spectra of (A) bPEI 12 kDa, (B) PEA and (C) BPEA.
Mentions: The successful generation of PEA and BPEA was further confirmed using FT-IR. In the spectrum of PEI (Fig. 3A), stretching vibration peaks of proton assigned to methyl and methylene could be found at 2920 cm−1 and 2822 cm−1. After cross-linking of PEI with propane-1,2,3-triol and due to the hydrogen bonding between N-H of PEI and C = O of propane-1,2,3-triol, an ester signal shifted to 1703 cm−1 in the spectrum of PEA (Fig. 3B). Finally, apart from the wide peak caused by the OH group at around 3200–3400 cm−1, O-C stretching vibration peaks of β-CD appeared at 1032, 1083 and1153 cm−1 in the spectrum of BPEA (Fig. 3C), suggesting successful incorporation of β-CD into PEA.

Bottom Line: This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery.In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity.Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT
Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

Show MeSH