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Combining neuroprotectants in a model of retinal degeneration: no additive benefit.

Di Marco F, Di Paolo M, Romeo S, Colecchi L, Fiorani L, Spana S, Stone J, Bisti S - PLoS ONE (2014)

Bottom Line: Results confirmed the neuroprotective potential of each used separately, but gave no evidence that their effects are additive.Detailed analysis suggests that there is actually a negative interaction between PBM and saffron when given simultaneously, with a consequent reduction of the neuroprotection.Specific testing will be required to understand the mechanisms involved and to establish whether there is clinical potential in combining neuroprotectants, to improve the quality of life of people affected by retinal pathology, such as age-related macular degeneration, the major cause of blindness and visual impairment in older adults.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Applied Clinical Science, University of L'Aquila, L'Aquila, Italy.

ABSTRACT
The central nervous system undergoing degeneration can be stabilized, and in some models can be restored to function, by neuroprotective treatments. Photobiomodulation (PBM) and dietary saffron are distinctive as neuroprotectants in that they upregulate protective mechanisms, without causing measurable tissue damage. This study reports a first attempt to combine the actions of PBM and saffron. Our working hypothesis was that the actions of PBM and saffron in protecting retinal photoreceptors, in a rat light damage model, would be additive. Results confirmed the neuroprotective potential of each used separately, but gave no evidence that their effects are additive. Detailed analysis suggests that there is actually a negative interaction between PBM and saffron when given simultaneously, with a consequent reduction of the neuroprotection. Specific testing will be required to understand the mechanisms involved and to establish whether there is clinical potential in combining neuroprotectants, to improve the quality of life of people affected by retinal pathology, such as age-related macular degeneration, the major cause of blindness and visual impairment in older adults.

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Impact of single and combined neuroprotectants on the thickness of the ONL in superior retina.A–E: Representative bisbenzimide labelling in control (A), light damage (B), 7 days PBM (C), 10 days saffron (D) and combined treatment (E) groups, 1 w after light damage. Images are taken one millimeter dorsal from optic disc. F: The ratio of ONL thickness to retinal thickness from the superior to the inferior edge. The arrow shows the position of the optic disc. Different symbols represent different experimental groups. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. G: The ratio of ONL thickness to retinal thickness, in the hot spot area (the area of greatest light-induced damage), 1 mm superior to the optic disc. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. Statistical significance indicators: (***) p<0.001; (**) p<0.01, for the difference of each group from the light damage group value.
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pone-0100389-g002: Impact of single and combined neuroprotectants on the thickness of the ONL in superior retina.A–E: Representative bisbenzimide labelling in control (A), light damage (B), 7 days PBM (C), 10 days saffron (D) and combined treatment (E) groups, 1 w after light damage. Images are taken one millimeter dorsal from optic disc. F: The ratio of ONL thickness to retinal thickness from the superior to the inferior edge. The arrow shows the position of the optic disc. Different symbols represent different experimental groups. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. G: The ratio of ONL thickness to retinal thickness, in the hot spot area (the area of greatest light-induced damage), 1 mm superior to the optic disc. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. Statistical significance indicators: (***) p<0.001; (**) p<0.01, for the difference of each group from the light damage group value.

Mentions: Figure 2 shows representative images of superior mid-peripheral retina, in a control retina (A), and in retinas from the four experimental groups (B-E). The ONL is 50-80 µm thick in the control retina (A), and is sharply reduced in thickness by exposure to bright light for 24 h (B). Preconditioning with PBM (C) or saffron (D) was protective, limiting the thinning on the ONL; this confirms prior reports [1], [4], [5], [14]. Conditioning with both PBM and saffron (E) was also protective, but did not improve survival, compared to saffron or PBM conditioning separately.


Combining neuroprotectants in a model of retinal degeneration: no additive benefit.

Di Marco F, Di Paolo M, Romeo S, Colecchi L, Fiorani L, Spana S, Stone J, Bisti S - PLoS ONE (2014)

Impact of single and combined neuroprotectants on the thickness of the ONL in superior retina.A–E: Representative bisbenzimide labelling in control (A), light damage (B), 7 days PBM (C), 10 days saffron (D) and combined treatment (E) groups, 1 w after light damage. Images are taken one millimeter dorsal from optic disc. F: The ratio of ONL thickness to retinal thickness from the superior to the inferior edge. The arrow shows the position of the optic disc. Different symbols represent different experimental groups. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. G: The ratio of ONL thickness to retinal thickness, in the hot spot area (the area of greatest light-induced damage), 1 mm superior to the optic disc. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. Statistical significance indicators: (***) p<0.001; (**) p<0.01, for the difference of each group from the light damage group value.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4067315&req=5

pone-0100389-g002: Impact of single and combined neuroprotectants on the thickness of the ONL in superior retina.A–E: Representative bisbenzimide labelling in control (A), light damage (B), 7 days PBM (C), 10 days saffron (D) and combined treatment (E) groups, 1 w after light damage. Images are taken one millimeter dorsal from optic disc. F: The ratio of ONL thickness to retinal thickness from the superior to the inferior edge. The arrow shows the position of the optic disc. Different symbols represent different experimental groups. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. G: The ratio of ONL thickness to retinal thickness, in the hot spot area (the area of greatest light-induced damage), 1 mm superior to the optic disc. For each group, each point shows the mean of the group; the error bars show standard errors of the mean. Statistical significance indicators: (***) p<0.001; (**) p<0.01, for the difference of each group from the light damage group value.
Mentions: Figure 2 shows representative images of superior mid-peripheral retina, in a control retina (A), and in retinas from the four experimental groups (B-E). The ONL is 50-80 µm thick in the control retina (A), and is sharply reduced in thickness by exposure to bright light for 24 h (B). Preconditioning with PBM (C) or saffron (D) was protective, limiting the thinning on the ONL; this confirms prior reports [1], [4], [5], [14]. Conditioning with both PBM and saffron (E) was also protective, but did not improve survival, compared to saffron or PBM conditioning separately.

Bottom Line: Results confirmed the neuroprotective potential of each used separately, but gave no evidence that their effects are additive.Detailed analysis suggests that there is actually a negative interaction between PBM and saffron when given simultaneously, with a consequent reduction of the neuroprotection.Specific testing will be required to understand the mechanisms involved and to establish whether there is clinical potential in combining neuroprotectants, to improve the quality of life of people affected by retinal pathology, such as age-related macular degeneration, the major cause of blindness and visual impairment in older adults.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Applied Clinical Science, University of L'Aquila, L'Aquila, Italy.

ABSTRACT
The central nervous system undergoing degeneration can be stabilized, and in some models can be restored to function, by neuroprotective treatments. Photobiomodulation (PBM) and dietary saffron are distinctive as neuroprotectants in that they upregulate protective mechanisms, without causing measurable tissue damage. This study reports a first attempt to combine the actions of PBM and saffron. Our working hypothesis was that the actions of PBM and saffron in protecting retinal photoreceptors, in a rat light damage model, would be additive. Results confirmed the neuroprotective potential of each used separately, but gave no evidence that their effects are additive. Detailed analysis suggests that there is actually a negative interaction between PBM and saffron when given simultaneously, with a consequent reduction of the neuroprotection. Specific testing will be required to understand the mechanisms involved and to establish whether there is clinical potential in combining neuroprotectants, to improve the quality of life of people affected by retinal pathology, such as age-related macular degeneration, the major cause of blindness and visual impairment in older adults.

Show MeSH
Related in: MedlinePlus