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DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.

Wang Y, Luo Y, Bing T, Chen Z, Lu M, Zhang N, Shangguan D, Gao X - PLoS ONE (2014)

Bottom Line: Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines.The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa.The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

ABSTRACT
Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.

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Related in: MedlinePlus

The binding of selected aptamer to PC-3 cells pre-treated with trypsin (A) or proteinase-K (B).After treatment with trypsin or proteinase K, PC-3 cells almost did not bind Wy-5a. Blank is the background fluorescence of untreated cells respectively.
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pone-0100243-g005: The binding of selected aptamer to PC-3 cells pre-treated with trypsin (A) or proteinase-K (B).After treatment with trypsin or proteinase K, PC-3 cells almost did not bind Wy-5a. Blank is the background fluorescence of untreated cells respectively.

Mentions: The confocal imaging has shown that the obtained aptamers bound on cell surface (Figure 2C and Figure 4 Row A), thus a proteinase digestion experiment was carried out to verify whether the target molecule is a membrane protein. As shown in Figure 5, after treatment with trypsin or proteinase K for 2 min, PC-3 cells almost did not bind Wy-5a, indicating that the target molecule of aptamer Wy-5 is a membrane protein.


DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.

Wang Y, Luo Y, Bing T, Chen Z, Lu M, Zhang N, Shangguan D, Gao X - PLoS ONE (2014)

The binding of selected aptamer to PC-3 cells pre-treated with trypsin (A) or proteinase-K (B).After treatment with trypsin or proteinase K, PC-3 cells almost did not bind Wy-5a. Blank is the background fluorescence of untreated cells respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4067300&req=5

pone-0100243-g005: The binding of selected aptamer to PC-3 cells pre-treated with trypsin (A) or proteinase-K (B).After treatment with trypsin or proteinase K, PC-3 cells almost did not bind Wy-5a. Blank is the background fluorescence of untreated cells respectively.
Mentions: The confocal imaging has shown that the obtained aptamers bound on cell surface (Figure 2C and Figure 4 Row A), thus a proteinase digestion experiment was carried out to verify whether the target molecule is a membrane protein. As shown in Figure 5, after treatment with trypsin or proteinase K for 2 min, PC-3 cells almost did not bind Wy-5a, indicating that the target molecule of aptamer Wy-5 is a membrane protein.

Bottom Line: Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines.The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa.The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, the third affiliated hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

ABSTRACT
Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.

Show MeSH
Related in: MedlinePlus