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Glutamate and lipid metabolic perturbation in the hippocampi of asymptomatic borna disease virus-infected horses.

Zhang L, Lei Y, Liu X, Wang X, Liu Z, Li D, Zheng P, Zhang L, Chen S, Xie P - PLoS ONE (2014)

Bottom Line: Multivariate statistical analysis showed a significant discrimination between the BDV-infected and control groups.These differential metabolites are primarily involved in glutamate and lipid metabolism.These finding provide an improved understanding of hippocampal changes associated with asymptomatic BDV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, China.

ABSTRACT
Borna disease virus (BDV) is a neurotropic, enveloped, non-segmented, negative-stranded RNA virus that infects a wide variety of vertebrate species from birds to humans across a broad global geographic distribution. Animal symptomatology range from asymptomatic infection to behavioral abnormalities to acute meningoencephalitis. Asymptomatic BDV infection has been shown to be more frequent than conventionally estimated. However, the molecular mechanism(s) underyling asymptomatic BDV infection remain largely unknown. Here, based on real-time quantitative PCR and Western blotting, a total of 18 horse hippocampi were divided into BDV-infected (n = 8) and non-infected control (n = 10) groups. A gas chromatography coupled with mass spectrometry (GC-MS) metabolomic approach, in conjunction with multivariate statistical analysis, was used to characterize the hippocampal metabolic changes associated with asymptomatic BDV infection. Multivariate statistical analysis showed a significant discrimination between the BDV-infected and control groups. BDV-infected hippocampi were characterized by lower levels of D-myo-inositol-1-phosphate, glutamate, phosphoethanolamine, heptadecanoic acid, and linoleic acid in combination with a higher level of ammonia. These differential metabolites are primarily involved in glutamate and lipid metabolism. These finding provide an improved understanding of hippocampal changes associated with asymptomatic BDV infection.

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Representative GC-MS Total Ion Current Chromatograms from BDV-Infected and Control Horses.
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pone-0099752-g002: Representative GC-MS Total Ion Current Chromatograms from BDV-Infected and Control Horses.

Mentions: Representative GC/MS total ion current (TIC) chromatograms of hippocampal samples from the BDV and control group are displayed in Fig. 2. Initially, we took advantage of 2D-PCA scores plot (principal components 1 versus principal components 4) in order to reflect the metabolic differences associated with BDV infection. Although the clusters of the BDV and control groups partially overlapped, distinct clustering of metabolic profiles was still obtained (Fig. 3a). The OPLS-DA model, a supervised projection method, verified a better class separation (Fig. 3b), and R2Y and Q2 demonstrated high robustness (R2Y = 0.918, Q2 = 0.732).


Glutamate and lipid metabolic perturbation in the hippocampi of asymptomatic borna disease virus-infected horses.

Zhang L, Lei Y, Liu X, Wang X, Liu Z, Li D, Zheng P, Zhang L, Chen S, Xie P - PLoS ONE (2014)

Representative GC-MS Total Ion Current Chromatograms from BDV-Infected and Control Horses.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4067290&req=5

pone-0099752-g002: Representative GC-MS Total Ion Current Chromatograms from BDV-Infected and Control Horses.
Mentions: Representative GC/MS total ion current (TIC) chromatograms of hippocampal samples from the BDV and control group are displayed in Fig. 2. Initially, we took advantage of 2D-PCA scores plot (principal components 1 versus principal components 4) in order to reflect the metabolic differences associated with BDV infection. Although the clusters of the BDV and control groups partially overlapped, distinct clustering of metabolic profiles was still obtained (Fig. 3a). The OPLS-DA model, a supervised projection method, verified a better class separation (Fig. 3b), and R2Y and Q2 demonstrated high robustness (R2Y = 0.918, Q2 = 0.732).

Bottom Line: Multivariate statistical analysis showed a significant discrimination between the BDV-infected and control groups.These differential metabolites are primarily involved in glutamate and lipid metabolism.These finding provide an improved understanding of hippocampal changes associated with asymptomatic BDV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, China.

ABSTRACT
Borna disease virus (BDV) is a neurotropic, enveloped, non-segmented, negative-stranded RNA virus that infects a wide variety of vertebrate species from birds to humans across a broad global geographic distribution. Animal symptomatology range from asymptomatic infection to behavioral abnormalities to acute meningoencephalitis. Asymptomatic BDV infection has been shown to be more frequent than conventionally estimated. However, the molecular mechanism(s) underyling asymptomatic BDV infection remain largely unknown. Here, based on real-time quantitative PCR and Western blotting, a total of 18 horse hippocampi were divided into BDV-infected (n = 8) and non-infected control (n = 10) groups. A gas chromatography coupled with mass spectrometry (GC-MS) metabolomic approach, in conjunction with multivariate statistical analysis, was used to characterize the hippocampal metabolic changes associated with asymptomatic BDV infection. Multivariate statistical analysis showed a significant discrimination between the BDV-infected and control groups. BDV-infected hippocampi were characterized by lower levels of D-myo-inositol-1-phosphate, glutamate, phosphoethanolamine, heptadecanoic acid, and linoleic acid in combination with a higher level of ammonia. These differential metabolites are primarily involved in glutamate and lipid metabolism. These finding provide an improved understanding of hippocampal changes associated with asymptomatic BDV infection.

Show MeSH
Related in: MedlinePlus