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Preparation of laponite bioceramics for potential bone tissue engineering applications.

Wang C, Wang S, Li K, Ju Y, Li J, Zhang Y, Li J, Liu X, Shi X, Zhao Q - PLoS ONE (2014)

Bottom Line: Our results reveal that the LAP bioceramic possesses an excellent surface hydrophilicity and serum absorption capacity, and good cytocompatibility and hemocompatibility as demonstrated by resazurin reduction assay of rat mesenchymal stem cells (rMSCs) and hemolytic assay of pig red blood cells, respectively.Most strikingly, alkaline phosphatase activity together with alizarin red staining results reveal that the produced LAP bioceramic is able to induce osteoblast differentiation of rMSCs in growth medium without any inducing factors.Finally, in vivo animal implantation, acute systemic toxicity test and hematoxylin and eosin (H&E)-staining data demonstrate that the prepared LAP bioceramic displays an excellent biosafety and is able to heal the bone defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P. R. China.

ABSTRACT
We report a facile approach to preparing laponite (LAP) bioceramics via sintering LAP powder compacts for bone tissue engineering applications. The sintering behavior and mechanical properties of LAP compacts under different temperatures, heating rates, and soaking times were investigated. We show that LAP bioceramic with a smooth and porous surface can be formed at 800°C with a heating rate of 5°C/h for 6 h under air. The formed LAP bioceramic was systematically characterized via different methods. Our results reveal that the LAP bioceramic possesses an excellent surface hydrophilicity and serum absorption capacity, and good cytocompatibility and hemocompatibility as demonstrated by resazurin reduction assay of rat mesenchymal stem cells (rMSCs) and hemolytic assay of pig red blood cells, respectively. The potential bone tissue engineering applicability of LAP bioceramic was explored by studying the surface mineralization behavior via soaking in simulated body fluid (SBF), as well as the surface cellular response of rMSCs. Our results suggest that LAP bioceramic is able to induce hydroxyapatite deposition on its surface when soaked in SBF and rMSCs can proliferate well on the LAP bioceramic surface. Most strikingly, alkaline phosphatase activity together with alizarin red staining results reveal that the produced LAP bioceramic is able to induce osteoblast differentiation of rMSCs in growth medium without any inducing factors. Finally, in vivo animal implantation, acute systemic toxicity test and hematoxylin and eosin (H&E)-staining data demonstrate that the prepared LAP bioceramic displays an excellent biosafety and is able to heal the bone defect. Findings from this study suggest that the developed LAP bioceramic holds a great promise for treating bone defects in bone tissue engineering.

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Biomineralization onto the surface of LAP ceramic.(a) and (c) show the SEM surface morphology of LAP ceramic before and after soaking in SBF solution for 7 days, respectively. (b) and (d) show the EDS analysis of the LAP ceramic before and after soaking in SBF solution for 7 days, respectively.
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pone-0099585-g005: Biomineralization onto the surface of LAP ceramic.(a) and (c) show the SEM surface morphology of LAP ceramic before and after soaking in SBF solution for 7 days, respectively. (b) and (d) show the EDS analysis of the LAP ceramic before and after soaking in SBF solution for 7 days, respectively.

Mentions: Bone-like HA plays an essential role in the formation, growth, and maintenance of the tissue-biomaterial interface [34]. Bioceramic was reported to be compatible with living bone through interfacial formation of HA interface layer [18]. We then evaluated the HA formation ability by soaking LAP bioceramic into SBF. Figures 5a and 5c illustrate the surface morphology of LAP ceramic before and after soaking in SBF solution for 7 days, respectively. Obviously, the HA deposits could be found on the LAP bioceremic surface after 7 day incubation in SBF. The mineralized HA shows a particulate morphology, with a diameter of nearly 1 µm. Figures 5b and 5d show the EDS analysis of the LAP ceramic before and after soaking in SBF solution for 7 days, respectively. It can be seen that before soaking in SBF, the element of Si, Mg, Na, and O belonging to the LAP itself can be detected. After mineralization for 7 days in SBF, besides the above elements associated with LAP, Ca and P with a molar ratio of 1.57 similar to the stoichiometric molar ratio of HA can be found, further confirming that LAP biocermic possesses the apatite formation ability, which is essential for its bone tissue engineering applications.


Preparation of laponite bioceramics for potential bone tissue engineering applications.

Wang C, Wang S, Li K, Ju Y, Li J, Zhang Y, Li J, Liu X, Shi X, Zhao Q - PLoS ONE (2014)

Biomineralization onto the surface of LAP ceramic.(a) and (c) show the SEM surface morphology of LAP ceramic before and after soaking in SBF solution for 7 days, respectively. (b) and (d) show the EDS analysis of the LAP ceramic before and after soaking in SBF solution for 7 days, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4067276&req=5

pone-0099585-g005: Biomineralization onto the surface of LAP ceramic.(a) and (c) show the SEM surface morphology of LAP ceramic before and after soaking in SBF solution for 7 days, respectively. (b) and (d) show the EDS analysis of the LAP ceramic before and after soaking in SBF solution for 7 days, respectively.
Mentions: Bone-like HA plays an essential role in the formation, growth, and maintenance of the tissue-biomaterial interface [34]. Bioceramic was reported to be compatible with living bone through interfacial formation of HA interface layer [18]. We then evaluated the HA formation ability by soaking LAP bioceramic into SBF. Figures 5a and 5c illustrate the surface morphology of LAP ceramic before and after soaking in SBF solution for 7 days, respectively. Obviously, the HA deposits could be found on the LAP bioceremic surface after 7 day incubation in SBF. The mineralized HA shows a particulate morphology, with a diameter of nearly 1 µm. Figures 5b and 5d show the EDS analysis of the LAP ceramic before and after soaking in SBF solution for 7 days, respectively. It can be seen that before soaking in SBF, the element of Si, Mg, Na, and O belonging to the LAP itself can be detected. After mineralization for 7 days in SBF, besides the above elements associated with LAP, Ca and P with a molar ratio of 1.57 similar to the stoichiometric molar ratio of HA can be found, further confirming that LAP biocermic possesses the apatite formation ability, which is essential for its bone tissue engineering applications.

Bottom Line: Our results reveal that the LAP bioceramic possesses an excellent surface hydrophilicity and serum absorption capacity, and good cytocompatibility and hemocompatibility as demonstrated by resazurin reduction assay of rat mesenchymal stem cells (rMSCs) and hemolytic assay of pig red blood cells, respectively.Most strikingly, alkaline phosphatase activity together with alizarin red staining results reveal that the produced LAP bioceramic is able to induce osteoblast differentiation of rMSCs in growth medium without any inducing factors.Finally, in vivo animal implantation, acute systemic toxicity test and hematoxylin and eosin (H&E)-staining data demonstrate that the prepared LAP bioceramic displays an excellent biosafety and is able to heal the bone defect.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, P. R. China.

ABSTRACT
We report a facile approach to preparing laponite (LAP) bioceramics via sintering LAP powder compacts for bone tissue engineering applications. The sintering behavior and mechanical properties of LAP compacts under different temperatures, heating rates, and soaking times were investigated. We show that LAP bioceramic with a smooth and porous surface can be formed at 800°C with a heating rate of 5°C/h for 6 h under air. The formed LAP bioceramic was systematically characterized via different methods. Our results reveal that the LAP bioceramic possesses an excellent surface hydrophilicity and serum absorption capacity, and good cytocompatibility and hemocompatibility as demonstrated by resazurin reduction assay of rat mesenchymal stem cells (rMSCs) and hemolytic assay of pig red blood cells, respectively. The potential bone tissue engineering applicability of LAP bioceramic was explored by studying the surface mineralization behavior via soaking in simulated body fluid (SBF), as well as the surface cellular response of rMSCs. Our results suggest that LAP bioceramic is able to induce hydroxyapatite deposition on its surface when soaked in SBF and rMSCs can proliferate well on the LAP bioceramic surface. Most strikingly, alkaline phosphatase activity together with alizarin red staining results reveal that the produced LAP bioceramic is able to induce osteoblast differentiation of rMSCs in growth medium without any inducing factors. Finally, in vivo animal implantation, acute systemic toxicity test and hematoxylin and eosin (H&E)-staining data demonstrate that the prepared LAP bioceramic displays an excellent biosafety and is able to heal the bone defect. Findings from this study suggest that the developed LAP bioceramic holds a great promise for treating bone defects in bone tissue engineering.

Show MeSH
Related in: MedlinePlus