Caveolin-1 alters the pattern of cytoplasmic Ca2+ oscillations and Ca2+-dependent gene expression by enhancing leukotriene receptor desensitization.
Bottom Line: Here, we show that the scaffolding protein caveolin-1 has a profound effect on receptor-driven Ca(2+) signals and downstream gene expression.Mutagenesis studies revealed that these effects required a functional scaffolding domain within caveolin-1.Our results reveal that caveolin-1 is a bimodal regulator of receptor-dependent Ca(2+) signaling, which fine-tunes the spatial and temporal profile of the Ca(2+) rise and thereby its ability to activate the NFAT pathway.
Affiliation: From the Department of Physiology, Anatomy, and Genetics, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom and.Show MeSH
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Mentions: Subcellular distribution of caveolin-1 and CysLT1 receptors in RBL-1 cells. Cells were co-transfected with caveolin-1-RFP and FLAG-tagged CysLT1 receptor and then fixed 48 h later. A, confocal images for the conditions shown. Line scans are shown in the merged panel. B, fluorescence profiles from the line scans are shown. Caveolin-1-RFP distribution is shown in red, and FLAG-tagged CysLT1 receptors are in green. C, histogram compares Pearson's correlation coefficient for the conditions shown. CaV-mutant denotes caveolin-1 with point mutations in the scaffolding domain (see Fig. 4). Mem., membrane; Cyt., cytosol.
Affiliation: From the Department of Physiology, Anatomy, and Genetics, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom and.