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Non-tuberculous mycobacteria have diverse effects on BCG efficacy against Mycobacterium tuberculosis.

Poyntz HC, Stylianou E, Griffiths KL, Marsay L, Checkley AM, McShane H - Tuberculosis (Edinb) (2014)

Bottom Line: The efficacy of Bacillus Calmette-Guerin (BCG) vaccination in protection against pulmonary tuberculosis (TB) is highly variable between populations.CD4+ and CD8+ T cell responses were profiled to define the immunological mechanisms underlying any effect on BCG efficacy.BCG efficacy was reduced by exposure to live MA administered by the oral route; T helper 2 cells were associated with reduced protection.

View Article: PubMed Central - PubMed

Affiliation: The Jenner Institute, Nuffield Department of Clinical Medicine, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, United Kingdom. Electronic address: hpoyntz@malaghan.org.nz.

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Burden of M. tb CFU in the lungs and spleens 35 days after challenge Groups in the challenge included naïve mice, BCG vaccinated mice (BCG) and BCG vaccinated followed by oral MA 104 exposure mice (BCG + MA 104). Data is Log (10) of the CFU count of the whole organ. Each data point represents one mouse and the mean is displayed. A One-way ANOVA with Bonferroni post test was used to determine statistical significance; the p value for each comparison is displayed. Colony forming units (CFU), 6 months since BCG (6 m).
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fig5: Burden of M. tb CFU in the lungs and spleens 35 days after challenge Groups in the challenge included naïve mice, BCG vaccinated mice (BCG) and BCG vaccinated followed by oral MA 104 exposure mice (BCG + MA 104). Data is Log (10) of the CFU count of the whole organ. Each data point represents one mouse and the mean is displayed. A One-way ANOVA with Bonferroni post test was used to determine statistical significance; the p value for each comparison is displayed. Colony forming units (CFU), 6 months since BCG (6 m).

Mentions: Thirty five days after aerosol M. tb challenge, the group that received MA 104 orally after BCG had a significantly greater CFU burden in the spleen compared to the BCG alone group (p = <0.05 spleen). The mean CFU count in the lung of the group receiving MA 104 orally after BCG was greater compared to the group that received BCG alone but this difference did not reach statistical significance. The mean Log(10) CFU counts in the naïve group was 5.62 lung; 4.36 spleen, in the BCG alone group 4.92 lung; 2.34 spleen and in the group that received MA 104 after BCG 5.1 lung; 3.63 spleen (Figure 5). Both the BCG alone group and the group receiving MA 104 orally after BCG had a significantly lower CFU burden in the lungs compared to naïve mice and the BCG alone group had significantly lower CFU burden in the spleen compared to naïve mice (p = <0.0001 lungs, p = <0.001 spleen BCG alone; p = <0.0001 lungs, p = ns spleen BCG – oral MA;Figure 5(A) and (B). The CFU burden in the spleen of the group receiving MA 104 orally after BCG was not significantly different to the naïve group.


Non-tuberculous mycobacteria have diverse effects on BCG efficacy against Mycobacterium tuberculosis.

Poyntz HC, Stylianou E, Griffiths KL, Marsay L, Checkley AM, McShane H - Tuberculosis (Edinb) (2014)

Burden of M. tb CFU in the lungs and spleens 35 days after challenge Groups in the challenge included naïve mice, BCG vaccinated mice (BCG) and BCG vaccinated followed by oral MA 104 exposure mice (BCG + MA 104). Data is Log (10) of the CFU count of the whole organ. Each data point represents one mouse and the mean is displayed. A One-way ANOVA with Bonferroni post test was used to determine statistical significance; the p value for each comparison is displayed. Colony forming units (CFU), 6 months since BCG (6 m).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4066954&req=5

fig5: Burden of M. tb CFU in the lungs and spleens 35 days after challenge Groups in the challenge included naïve mice, BCG vaccinated mice (BCG) and BCG vaccinated followed by oral MA 104 exposure mice (BCG + MA 104). Data is Log (10) of the CFU count of the whole organ. Each data point represents one mouse and the mean is displayed. A One-way ANOVA with Bonferroni post test was used to determine statistical significance; the p value for each comparison is displayed. Colony forming units (CFU), 6 months since BCG (6 m).
Mentions: Thirty five days after aerosol M. tb challenge, the group that received MA 104 orally after BCG had a significantly greater CFU burden in the spleen compared to the BCG alone group (p = <0.05 spleen). The mean CFU count in the lung of the group receiving MA 104 orally after BCG was greater compared to the group that received BCG alone but this difference did not reach statistical significance. The mean Log(10) CFU counts in the naïve group was 5.62 lung; 4.36 spleen, in the BCG alone group 4.92 lung; 2.34 spleen and in the group that received MA 104 after BCG 5.1 lung; 3.63 spleen (Figure 5). Both the BCG alone group and the group receiving MA 104 orally after BCG had a significantly lower CFU burden in the lungs compared to naïve mice and the BCG alone group had significantly lower CFU burden in the spleen compared to naïve mice (p = <0.0001 lungs, p = <0.001 spleen BCG alone; p = <0.0001 lungs, p = ns spleen BCG – oral MA;Figure 5(A) and (B). The CFU burden in the spleen of the group receiving MA 104 orally after BCG was not significantly different to the naïve group.

Bottom Line: The efficacy of Bacillus Calmette-Guerin (BCG) vaccination in protection against pulmonary tuberculosis (TB) is highly variable between populations.CD4+ and CD8+ T cell responses were profiled to define the immunological mechanisms underlying any effect on BCG efficacy.BCG efficacy was reduced by exposure to live MA administered by the oral route; T helper 2 cells were associated with reduced protection.

View Article: PubMed Central - PubMed

Affiliation: The Jenner Institute, Nuffield Department of Clinical Medicine, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, United Kingdom. Electronic address: hpoyntz@malaghan.org.nz.

Show MeSH
Related in: MedlinePlus