Non-tuberculous mycobacteria have diverse effects on BCG efficacy against Mycobacterium tuberculosis.
Bottom Line: The efficacy of Bacillus Calmette-Guerin (BCG) vaccination in protection against pulmonary tuberculosis (TB) is highly variable between populations.CD4+ and CD8+ T cell responses were profiled to define the immunological mechanisms underlying any effect on BCG efficacy.BCG efficacy was reduced by exposure to live MA administered by the oral route; T helper 2 cells were associated with reduced protection.
Affiliation: The Jenner Institute, Nuffield Department of Clinical Medicine, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, United Kingdom. Electronic address: email@example.com.Show MeSH
Related in: MedlinePlus
Mentions: Frozen aliquots of bacteria were thawed and sonicated. Where MA was to be given heat-killed, the aliquots were placed in a water bath at 80 °C for 25 min. Bacteria were then diluted to the required concentration in sterile PBS before administration. 100 μl of BCG in PBS was injected subcutaneously (SC) at the base of the tail using a U-100 29G syringe. 1 × 105 CFU was administered. 100 μl of MA 724 or 2-151 for intraperitoneal injection (IP) was suspended in PBS and administered with a U-100 29G syringe in the right or left side of the peritoneal cavity. In the IP exposure model 1 × 108 CFU of killed MA strain 2-151 or 724 were administered. In the model of oral exposure mice received 100 μl of live MA 104 suspended in PBS via a dosing cannula inserted directly in to the stomach. 1 × 102 CFU was administered. Mice were restrained but not anaesthetised for these procedures (Figure 1(A): Experiment plan of the IP exposure model; Figure 4(A): Experiment plan of the oral exposure model).
Affiliation: The Jenner Institute, Nuffield Department of Clinical Medicine, University of Oxford, Old Road Campus Research Building, Oxford OX3 7DQ, United Kingdom. Electronic address: firstname.lastname@example.org.