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Observations on morphologic and genetic diversity in populations of Filoboletus manipularis (Fungi: Mycenaceae) in southern Viet Nam.

Vydryakova GA, Morozova OV, Redhead SA, Bissett J - Mycology (2014)

Bottom Line: No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied.The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations.On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition.

View Article: PubMed Central - PubMed

Affiliation: ECORC, Agriculture and Agri-Food Canada, Ottawa, Canada ; Vietnam-Russian Tropical Research and Technological Centre, Southern Branch, Ho Chi Minh City, Viet Nam.

ABSTRACT
The morphological variation of basidiomata of Filoboletus manipularis (Berk) Singer collected in southern Viet Nam was studied. Phylogenetic analyses comprising three gene loci indicated that these collections, although exhibiting widely varying morphologies, represented a single species with a population composed of genetically diverse, sexually compatible monokaryon parental strains. No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied. Primers were designed to amplify the intron-rich 5' region of the translation elongation factor 1-α gene (tef1α) and amplicons cloned and sequenced to characterize the parental haplotypes for individual basidiomata. The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations. On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition. The literature supporting our observations of the occurrence of multinucleate basidiomata is reviewed and possible mechanisms for this phenomenon are proposed.

No MeSH data available.


Split decomposition analysis of the tef1α locus. Pairwise homoplasy index (PHI) = 3.646–11, indicating the presence of recombination across all collections.
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Figure 13: Split decomposition analysis of the tef1α locus. Pairwise homoplasy index (PHI) = 3.646–11, indicating the presence of recombination across all collections.

Mentions: The phylogenetic analysis based on tef1α exon regions with Mycena plumbea (GU187729) as outgroup (Figure 11, http://purl.org/phylo/treebase/phylows/study/TB2:S14862) indicated considerable genetic distance between Filoboletus and Mycena with limited phylogenetic structure within F. manipularis. Analysis of gene diversities showed an unusually low level of heterozygosity among populations from the three different localities within the study area (Fst = −0.023, σ = 0.060, supplementary Table S1) indicating an absence of allelic fixation or inbreeding within localized populations. In the phylogenetic analysis fifteen clone sequence variants were grouped together in a polytomy collapsed at the 50% significance level. Among these, one clone variant (i.e. haplotype representing one presumed parental monokaryon) from G2 had identical exon sequence to one haplotype from each of G7 and G30, and one haplotype from G3 was identical to two haplotypes from G31. Similarly, Gl and G7 had one haplotype with identical exon sequences. However, none of the basidiomata had identical paired haplotypes, indicating a rich assemblage of compatible mating strains in relatively close proximity to one another in these locations. Only two branches within Filoboletus were supported at levels higher than 90% and none higher than 95%. One branch joined two haplotypes from G3, and the second joined haplotypes from G1 and G7. However, all three basidiomata had one additional haplotype outside of the supported branches. Significantly, three different haplotype sequences were seen for basidiomata of G3, G25 and G30 (supplementary Table S1). Since DNA for this study was isolated from individual basidiomata, which are presumed to have arisen from conjugation of two compatible monokaryons, this indicates that more than two haploid components could be involved in the development of individual basidiomata, Similar observations were found when the intron regions were included in the analysis (Figure 12, http://purl.org/phylo/treebase/phylows/study/TB2:S14862). Significant branches (>0.95) joined paired haplotypes for G31, G25 and G3, as well as for individual haplotypes from G1 with G7, and G2 with G24. With the exception of G31, the other basidiomata had additional haplotypes arising on unsupported branches (<0.95), again indicating the possibility that more than two component nuclei were involved in the development of individual basidiomata. G7 and G30 each had one haplotype with identical sequence, as did G1 and G7; however, all of these collections originated from the vicinity of Nam Cat Tiên. Recombination patterns were visualized by split decomposition analysis (Figure 13) onto which the morphological characteristics of the basidiomata were summarized. This analysis showed a complex network of relationships and statistically significant recombination within all of F. manipularis (Phitest, Φ = 3.64E-11). There was no evident pattern of phylogenetic differentiation or correlation of genetic variation with morphological characteristics.


Observations on morphologic and genetic diversity in populations of Filoboletus manipularis (Fungi: Mycenaceae) in southern Viet Nam.

Vydryakova GA, Morozova OV, Redhead SA, Bissett J - Mycology (2014)

Split decomposition analysis of the tef1α locus. Pairwise homoplasy index (PHI) = 3.646–11, indicating the presence of recombination across all collections.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4066922&req=5

Figure 13: Split decomposition analysis of the tef1α locus. Pairwise homoplasy index (PHI) = 3.646–11, indicating the presence of recombination across all collections.
Mentions: The phylogenetic analysis based on tef1α exon regions with Mycena plumbea (GU187729) as outgroup (Figure 11, http://purl.org/phylo/treebase/phylows/study/TB2:S14862) indicated considerable genetic distance between Filoboletus and Mycena with limited phylogenetic structure within F. manipularis. Analysis of gene diversities showed an unusually low level of heterozygosity among populations from the three different localities within the study area (Fst = −0.023, σ = 0.060, supplementary Table S1) indicating an absence of allelic fixation or inbreeding within localized populations. In the phylogenetic analysis fifteen clone sequence variants were grouped together in a polytomy collapsed at the 50% significance level. Among these, one clone variant (i.e. haplotype representing one presumed parental monokaryon) from G2 had identical exon sequence to one haplotype from each of G7 and G30, and one haplotype from G3 was identical to two haplotypes from G31. Similarly, Gl and G7 had one haplotype with identical exon sequences. However, none of the basidiomata had identical paired haplotypes, indicating a rich assemblage of compatible mating strains in relatively close proximity to one another in these locations. Only two branches within Filoboletus were supported at levels higher than 90% and none higher than 95%. One branch joined two haplotypes from G3, and the second joined haplotypes from G1 and G7. However, all three basidiomata had one additional haplotype outside of the supported branches. Significantly, three different haplotype sequences were seen for basidiomata of G3, G25 and G30 (supplementary Table S1). Since DNA for this study was isolated from individual basidiomata, which are presumed to have arisen from conjugation of two compatible monokaryons, this indicates that more than two haploid components could be involved in the development of individual basidiomata, Similar observations were found when the intron regions were included in the analysis (Figure 12, http://purl.org/phylo/treebase/phylows/study/TB2:S14862). Significant branches (>0.95) joined paired haplotypes for G31, G25 and G3, as well as for individual haplotypes from G1 with G7, and G2 with G24. With the exception of G31, the other basidiomata had additional haplotypes arising on unsupported branches (<0.95), again indicating the possibility that more than two component nuclei were involved in the development of individual basidiomata. G7 and G30 each had one haplotype with identical sequence, as did G1 and G7; however, all of these collections originated from the vicinity of Nam Cat Tiên. Recombination patterns were visualized by split decomposition analysis (Figure 13) onto which the morphological characteristics of the basidiomata were summarized. This analysis showed a complex network of relationships and statistically significant recombination within all of F. manipularis (Phitest, Φ = 3.64E-11). There was no evident pattern of phylogenetic differentiation or correlation of genetic variation with morphological characteristics.

Bottom Line: No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied.The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations.On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition.

View Article: PubMed Central - PubMed

Affiliation: ECORC, Agriculture and Agri-Food Canada, Ottawa, Canada ; Vietnam-Russian Tropical Research and Technological Centre, Southern Branch, Ho Chi Minh City, Viet Nam.

ABSTRACT
The morphological variation of basidiomata of Filoboletus manipularis (Berk) Singer collected in southern Viet Nam was studied. Phylogenetic analyses comprising three gene loci indicated that these collections, although exhibiting widely varying morphologies, represented a single species with a population composed of genetically diverse, sexually compatible monokaryon parental strains. No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied. Primers were designed to amplify the intron-rich 5' region of the translation elongation factor 1-α gene (tef1α) and amplicons cloned and sequenced to characterize the parental haplotypes for individual basidiomata. The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations. On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition. The literature supporting our observations of the occurrence of multinucleate basidiomata is reviewed and possible mechanisms for this phenomenon are proposed.

No MeSH data available.