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Observations on morphologic and genetic diversity in populations of Filoboletus manipularis (Fungi: Mycenaceae) in southern Viet Nam.

Vydryakova GA, Morozova OV, Redhead SA, Bissett J - Mycology (2014)

Bottom Line: No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied.The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations.On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition.

View Article: PubMed Central - PubMed

Affiliation: ECORC, Agriculture and Agri-Food Canada, Ottawa, Canada ; Vietnam-Russian Tropical Research and Technological Centre, Southern Branch, Ho Chi Minh City, Viet Nam.

ABSTRACT
The morphological variation of basidiomata of Filoboletus manipularis (Berk) Singer collected in southern Viet Nam was studied. Phylogenetic analyses comprising three gene loci indicated that these collections, although exhibiting widely varying morphologies, represented a single species with a population composed of genetically diverse, sexually compatible monokaryon parental strains. No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied. Primers were designed to amplify the intron-rich 5' region of the translation elongation factor 1-α gene (tef1α) and amplicons cloned and sequenced to characterize the parental haplotypes for individual basidiomata. The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations. On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition. The literature supporting our observations of the occurrence of multinucleate basidiomata is reviewed and possible mechanisms for this phenomenon are proposed.

No MeSH data available.


Median-joining network, showing polymorphisms in the internal transcribed spacer (ITS) regions. Lines separating ITS haplotypes represent polymorphisms: (a) adenosine indel at position 195, (b) T/C transition at position 338, (c) adenosine indel at position 538, (d) A/G transition at position 669; * alternate possible positions for AB509828 from Japan.
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Figure 10: Median-joining network, showing polymorphisms in the internal transcribed spacer (ITS) regions. Lines separating ITS haplotypes represent polymorphisms: (a) adenosine indel at position 195, (b) T/C transition at position 338, (c) adenosine indel at position 538, (d) A/G transition at position 669; * alternate possible positions for AB509828 from Japan.

Mentions: ITS was sequenced for 13 F. manipularis collections, with the ITS1-5.8S-ITS2 region varying from 667 to 669 nt in length. Four sequence polymorphisms were found in the ITS regions from the sequence alignment (Table 4). These were an adenosine indel at position 195 and a T-C transition at position 338 in the ITS1 region, and a second adenosine indel at position 538 and A-G transition at position 669 in the ITS2 region. The 5.8 region and bordering fragments of 28S and 18S were invariant in our samples. Two sequence variants were noted for collections G3, G7, G24 and G25 and unbalanced indels occurred in sequences for G3, G24 and G25, indicating ITS sequence differences including length variants between haplotypes from individual basidiomata in these collections. In total, there were 10 ITS sequence variants noted for all of the collections and median-joining analysis of the polymorphism similarities indicated an interconnected, non-branching genetic relationship among all presumed dikaryon strains (Figure 10). The ITS2 sequence for F. manipularis from Japan (Genbank AB509828) was identical to sequences for eight of the Viet Nam collections indicating a close genetic relationship over a broader geographic region than sampled in the current study.


Observations on morphologic and genetic diversity in populations of Filoboletus manipularis (Fungi: Mycenaceae) in southern Viet Nam.

Vydryakova GA, Morozova OV, Redhead SA, Bissett J - Mycology (2014)

Median-joining network, showing polymorphisms in the internal transcribed spacer (ITS) regions. Lines separating ITS haplotypes represent polymorphisms: (a) adenosine indel at position 195, (b) T/C transition at position 338, (c) adenosine indel at position 538, (d) A/G transition at position 669; * alternate possible positions for AB509828 from Japan.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4066922&req=5

Figure 10: Median-joining network, showing polymorphisms in the internal transcribed spacer (ITS) regions. Lines separating ITS haplotypes represent polymorphisms: (a) adenosine indel at position 195, (b) T/C transition at position 338, (c) adenosine indel at position 538, (d) A/G transition at position 669; * alternate possible positions for AB509828 from Japan.
Mentions: ITS was sequenced for 13 F. manipularis collections, with the ITS1-5.8S-ITS2 region varying from 667 to 669 nt in length. Four sequence polymorphisms were found in the ITS regions from the sequence alignment (Table 4). These were an adenosine indel at position 195 and a T-C transition at position 338 in the ITS1 region, and a second adenosine indel at position 538 and A-G transition at position 669 in the ITS2 region. The 5.8 region and bordering fragments of 28S and 18S were invariant in our samples. Two sequence variants were noted for collections G3, G7, G24 and G25 and unbalanced indels occurred in sequences for G3, G24 and G25, indicating ITS sequence differences including length variants between haplotypes from individual basidiomata in these collections. In total, there were 10 ITS sequence variants noted for all of the collections and median-joining analysis of the polymorphism similarities indicated an interconnected, non-branching genetic relationship among all presumed dikaryon strains (Figure 10). The ITS2 sequence for F. manipularis from Japan (Genbank AB509828) was identical to sequences for eight of the Viet Nam collections indicating a close genetic relationship over a broader geographic region than sampled in the current study.

Bottom Line: No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied.The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations.On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition.

View Article: PubMed Central - PubMed

Affiliation: ECORC, Agriculture and Agri-Food Canada, Ottawa, Canada ; Vietnam-Russian Tropical Research and Technological Centre, Southern Branch, Ho Chi Minh City, Viet Nam.

ABSTRACT
The morphological variation of basidiomata of Filoboletus manipularis (Berk) Singer collected in southern Viet Nam was studied. Phylogenetic analyses comprising three gene loci indicated that these collections, although exhibiting widely varying morphologies, represented a single species with a population composed of genetically diverse, sexually compatible monokaryon parental strains. No correlation was found between any aspect of morphological variation and intraspecific phylogenetic patterns for the three gene loci studied. Primers were designed to amplify the intron-rich 5' region of the translation elongation factor 1-α gene (tef1α) and amplicons cloned and sequenced to characterize the parental haplotypes for individual basidiomata. The presence of recombination over the entire morphological diversity seen was confirmed by split decomposition analysis and analysis of gene diversity indicated a lack of allelic fixation within local populations. On several occasions, more than two apparent parental haplotypes were characterized from individual basidiomata, indicating that at least some basidiomata are chimeric or otherwise develop from a multinucleate condition. The literature supporting our observations of the occurrence of multinucleate basidiomata is reviewed and possible mechanisms for this phenomenon are proposed.

No MeSH data available.