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Evaluation of plant phenolic metabolites as a source of Alzheimer's drug leads.

Hassaan Y, Handoussa H, El-Khatib AH, Linscheid MW, El Sayed N, Ayoub N - Biomed Res Int (2014)

Bottom Line: The aim of this study is pharmacological screening of the aqueous alcohol extract of Markhamia platycalyx leaves, Schotia brachypetala leaves and stalks, and piceatannol compared to aqueous alcohol extract of Camellia sinensis leaves as potential Alzheimer's disease drugs.LC-HRESI(-ve)-MS(n) was performed to identify phenolics' profile of Schotia brachypetala stalks aqueous alcohol extract and revealed ten phenolic compounds as first report: daidzein, naringin, procyanidin isomers, procyanidin dimer gallate, quercetin 3-O-rhamnoside, quercetin 3-O-glucuronide, quercetin hexose gallic acid, quercetin hexose protocatechuic acid, and ellagic acid.In vivo behavioral tests (Y maze and object recognition) and in vitro estimation of amyloid beta 42 by ELISA showed significant differences between results of treated and nontreated animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.

ABSTRACT
Epidemiological studies have proven an association between consumption of polyphenols and prevention of Alzheimer's disease, the most common form of dementia characterized by extracellular deposition of amyloid beta plaques. The aim of this study is pharmacological screening of the aqueous alcohol extract of Markhamia platycalyx leaves, Schotia brachypetala leaves and stalks, and piceatannol compared to aqueous alcohol extract of Camellia sinensis leaves as potential Alzheimer's disease drugs. LC-HRESI(-ve)-MS(n) was performed to identify phenolics' profile of Schotia brachypetala stalks aqueous alcohol extract and revealed ten phenolic compounds as first report: daidzein, naringin, procyanidin isomers, procyanidin dimer gallate, quercetin 3-O-rhamnoside, quercetin 3-O-glucuronide, quercetin hexose gallic acid, quercetin hexose protocatechuic acid, and ellagic acid. Alzheimer's disease was induced by a single intraperitoneal injection of LPS. Adult male Swiss albino mice were divided into groups of 8-10 mice each receiving treatment for six days. In vivo behavioral tests (Y maze and object recognition) and in vitro estimation of amyloid beta 42 by ELISA showed significant differences between results of treated and nontreated animals.

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Effects of Lipopolysaccharide, green tea, piceatannol, Markhamia platycalyx, and Schotia brachypetala leaves and Schotia brachypetala stalks on mean alternation percentage using Y maze test. Normal group animals were injected with DMSO or saline 0.9% i.p. once with nonsignificant results between them. The 6 other groups had Alzheimer's disease that was induced by i.p. injection of LPS once. Five of these groups were treated with either PCT (2.5 mg/kg), or 100 mg/kg of GT, MP, SBS, or SBL daily for 6 days. The animals of each group (n = 8–10) were subjected to Y maze testing on the 7th day and the sequence of arm entries was recorded for 8 minutes for every mouse. Then the percentage alternations were calculated for each mouse. Statistical analysis was performed using unpaired t-test to compare every two groups. Each value represents mean ± standard error of mean. *Significantly different from normal at P < 0.05.**Significantly different from normal at P < 0.01. ***Significantly different from normal at P < 0.001. +Significantly different from LPS at P < 0.05. ++Significantly different from LPS at P < 0.01. +++Significantly different from LPS at P < 0.001. MPA: mean alternation percentage. DMSO: dimethyl sulfoxide. LPS: Lipopolysaccharide. GT: green tea extract of Camellia sinensis. PCT: piceatannol. SBS: Schotia brachypetala leaves. SBL: Schotia brachypetala stalk. MP: Markhamia platycalyx. i.p. intraperitoneal injection.
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fig2: Effects of Lipopolysaccharide, green tea, piceatannol, Markhamia platycalyx, and Schotia brachypetala leaves and Schotia brachypetala stalks on mean alternation percentage using Y maze test. Normal group animals were injected with DMSO or saline 0.9% i.p. once with nonsignificant results between them. The 6 other groups had Alzheimer's disease that was induced by i.p. injection of LPS once. Five of these groups were treated with either PCT (2.5 mg/kg), or 100 mg/kg of GT, MP, SBS, or SBL daily for 6 days. The animals of each group (n = 8–10) were subjected to Y maze testing on the 7th day and the sequence of arm entries was recorded for 8 minutes for every mouse. Then the percentage alternations were calculated for each mouse. Statistical analysis was performed using unpaired t-test to compare every two groups. Each value represents mean ± standard error of mean. *Significantly different from normal at P < 0.05.**Significantly different from normal at P < 0.01. ***Significantly different from normal at P < 0.001. +Significantly different from LPS at P < 0.05. ++Significantly different from LPS at P < 0.01. +++Significantly different from LPS at P < 0.001. MPA: mean alternation percentage. DMSO: dimethyl sulfoxide. LPS: Lipopolysaccharide. GT: green tea extract of Camellia sinensis. PCT: piceatannol. SBS: Schotia brachypetala leaves. SBL: Schotia brachypetala stalk. MP: Markhamia platycalyx. i.p. intraperitoneal injection.

Mentions: Administration of LPS (0.8 mg/kg i.p) then on the 7th day subjecting mice to Y maze showed a significant decrease in the mean percentage alternations by 42.25% compared to normal group. The mean percentage alternations were significantly increased in animals that were treated with piceatannol (2.5 mg/kg/day), Camellia sinensis leaves (100 mg/kg/day), Markhamia platycalyx leaves (100 mg/kg/day), Schotia brachypetala leaves (100 mg/kg/day), and Schotia brachypetala stalk (100 mg/kg/day) for 6 days by 68.78%, 39.74%, 72.14%, 40.9%, and 57.85%, respectively, compared to LPS group (Figure 2).


Evaluation of plant phenolic metabolites as a source of Alzheimer's drug leads.

Hassaan Y, Handoussa H, El-Khatib AH, Linscheid MW, El Sayed N, Ayoub N - Biomed Res Int (2014)

Effects of Lipopolysaccharide, green tea, piceatannol, Markhamia platycalyx, and Schotia brachypetala leaves and Schotia brachypetala stalks on mean alternation percentage using Y maze test. Normal group animals were injected with DMSO or saline 0.9% i.p. once with nonsignificant results between them. The 6 other groups had Alzheimer's disease that was induced by i.p. injection of LPS once. Five of these groups were treated with either PCT (2.5 mg/kg), or 100 mg/kg of GT, MP, SBS, or SBL daily for 6 days. The animals of each group (n = 8–10) were subjected to Y maze testing on the 7th day and the sequence of arm entries was recorded for 8 minutes for every mouse. Then the percentage alternations were calculated for each mouse. Statistical analysis was performed using unpaired t-test to compare every two groups. Each value represents mean ± standard error of mean. *Significantly different from normal at P < 0.05.**Significantly different from normal at P < 0.01. ***Significantly different from normal at P < 0.001. +Significantly different from LPS at P < 0.05. ++Significantly different from LPS at P < 0.01. +++Significantly different from LPS at P < 0.001. MPA: mean alternation percentage. DMSO: dimethyl sulfoxide. LPS: Lipopolysaccharide. GT: green tea extract of Camellia sinensis. PCT: piceatannol. SBS: Schotia brachypetala leaves. SBL: Schotia brachypetala stalk. MP: Markhamia platycalyx. i.p. intraperitoneal injection.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig2: Effects of Lipopolysaccharide, green tea, piceatannol, Markhamia platycalyx, and Schotia brachypetala leaves and Schotia brachypetala stalks on mean alternation percentage using Y maze test. Normal group animals were injected with DMSO or saline 0.9% i.p. once with nonsignificant results between them. The 6 other groups had Alzheimer's disease that was induced by i.p. injection of LPS once. Five of these groups were treated with either PCT (2.5 mg/kg), or 100 mg/kg of GT, MP, SBS, or SBL daily for 6 days. The animals of each group (n = 8–10) were subjected to Y maze testing on the 7th day and the sequence of arm entries was recorded for 8 minutes for every mouse. Then the percentage alternations were calculated for each mouse. Statistical analysis was performed using unpaired t-test to compare every two groups. Each value represents mean ± standard error of mean. *Significantly different from normal at P < 0.05.**Significantly different from normal at P < 0.01. ***Significantly different from normal at P < 0.001. +Significantly different from LPS at P < 0.05. ++Significantly different from LPS at P < 0.01. +++Significantly different from LPS at P < 0.001. MPA: mean alternation percentage. DMSO: dimethyl sulfoxide. LPS: Lipopolysaccharide. GT: green tea extract of Camellia sinensis. PCT: piceatannol. SBS: Schotia brachypetala leaves. SBL: Schotia brachypetala stalk. MP: Markhamia platycalyx. i.p. intraperitoneal injection.
Mentions: Administration of LPS (0.8 mg/kg i.p) then on the 7th day subjecting mice to Y maze showed a significant decrease in the mean percentage alternations by 42.25% compared to normal group. The mean percentage alternations were significantly increased in animals that were treated with piceatannol (2.5 mg/kg/day), Camellia sinensis leaves (100 mg/kg/day), Markhamia platycalyx leaves (100 mg/kg/day), Schotia brachypetala leaves (100 mg/kg/day), and Schotia brachypetala stalk (100 mg/kg/day) for 6 days by 68.78%, 39.74%, 72.14%, 40.9%, and 57.85%, respectively, compared to LPS group (Figure 2).

Bottom Line: The aim of this study is pharmacological screening of the aqueous alcohol extract of Markhamia platycalyx leaves, Schotia brachypetala leaves and stalks, and piceatannol compared to aqueous alcohol extract of Camellia sinensis leaves as potential Alzheimer's disease drugs.LC-HRESI(-ve)-MS(n) was performed to identify phenolics' profile of Schotia brachypetala stalks aqueous alcohol extract and revealed ten phenolic compounds as first report: daidzein, naringin, procyanidin isomers, procyanidin dimer gallate, quercetin 3-O-rhamnoside, quercetin 3-O-glucuronide, quercetin hexose gallic acid, quercetin hexose protocatechuic acid, and ellagic acid.In vivo behavioral tests (Y maze and object recognition) and in vitro estimation of amyloid beta 42 by ELISA showed significant differences between results of treated and nontreated animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.

ABSTRACT
Epidemiological studies have proven an association between consumption of polyphenols and prevention of Alzheimer's disease, the most common form of dementia characterized by extracellular deposition of amyloid beta plaques. The aim of this study is pharmacological screening of the aqueous alcohol extract of Markhamia platycalyx leaves, Schotia brachypetala leaves and stalks, and piceatannol compared to aqueous alcohol extract of Camellia sinensis leaves as potential Alzheimer's disease drugs. LC-HRESI(-ve)-MS(n) was performed to identify phenolics' profile of Schotia brachypetala stalks aqueous alcohol extract and revealed ten phenolic compounds as first report: daidzein, naringin, procyanidin isomers, procyanidin dimer gallate, quercetin 3-O-rhamnoside, quercetin 3-O-glucuronide, quercetin hexose gallic acid, quercetin hexose protocatechuic acid, and ellagic acid. Alzheimer's disease was induced by a single intraperitoneal injection of LPS. Adult male Swiss albino mice were divided into groups of 8-10 mice each receiving treatment for six days. In vivo behavioral tests (Y maze and object recognition) and in vitro estimation of amyloid beta 42 by ELISA showed significant differences between results of treated and nontreated animals.

Show MeSH
Related in: MedlinePlus