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Potential of Pleurotus ostreatus mycelium for selenium absorption.

Milovanović I, Brčeski I, Stajić M, Korać A, Vukojević J, Knežević A - ScientificWorldJournal (2014)

Bottom Line: The aim of this study was to evaluate the effect of high selenium (Se) concentrations on morphophysiological and ultrastructural properties of Pleurotus ostreatus.Biomass yield ranged between 11.8 g L(-1), in the control, and 6.8 g L(-1), at an Se concentration of 100.0 mg L(-1), while no production was detected at a concentration of 500.0 mg L(-1).Se content in the mycelia reached a peak (938.9 μg g(-1)) after cultivation in the medium enriched with Se at the concentration of 20.0 mg L(-1), while the highest absorption level (53.25%) was found in the medium enriched with 5.0 mg L(-1) Se.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia.

ABSTRACT
The aim of this study was to evaluate the effect of high selenium (Se) concentrations on morphophysiological and ultrastructural properties of Pleurotus ostreatus. Mycelium growth was good in media enriched with 5.0, 10.0, and 20.0 mg L(-1) of Se, concentration of 500.0 mg L(-1) strongly inhibited growth, and 1000.0 mg L(-1) was the minimum inhibitory concentration. Contrary to thin-walled, hyaline, branched, and anastomized hyphae with clamp-connections in the control, at Se concentrations of 100.0 and 500.0 mg L(-1), they were noticeably short, frequently septed and branched, with a more intensive extracellular matrix, and without clamp-connections. At high Se concentrations, hyphae with intact membrane, without cellular contents, with a high level of vacuolization, and with numerous proteinaceous bodies were observed. Biomass yield ranged between 11.8 g L(-1), in the control, and 6.8 g L(-1), at an Se concentration of 100.0 mg L(-1), while no production was detected at a concentration of 500.0 mg L(-1). Se content in the mycelia reached a peak (938.9 μg g(-1)) after cultivation in the medium enriched with Se at the concentration of 20.0 mg L(-1), while the highest absorption level (53.25%) was found in the medium enriched with 5.0 mg L(-1) Se.

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Se metabolism pathways (scheme).
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Related In: Results  -  Collection


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fig2: Se metabolism pathways (scheme).

Mentions: Although it is known that absorbed Se is incorporated into selenocysteine, selenomethionine, Se-methylselenocysteine, and several unidentified selenocompounds [29], mechanisms of its uptake, translocation, accumulation, and metabolism depend on the species [30]. According to Ip [27] and Lenz and Lens [2], water soluble selenites and selenates could be introduced into two metabolic pathways: (i) methylation directly or indirectly (through incorporation into selenomethionine) to methylselenol, dimethyl selenide, and trimethylselenonium ion which are vaporized or excreted and (ii) reduction to insoluble Se0, which could be either reoxidized to selenites, rarely selenates, or reduced to H2Se. H2Se, a precursor for selenoprotein synthesis, in the reaction with metal cations forms metal-Se that further either precipitates or oxidizes to selenites (Figure 2).


Potential of Pleurotus ostreatus mycelium for selenium absorption.

Milovanović I, Brčeski I, Stajić M, Korać A, Vukojević J, Knežević A - ScientificWorldJournal (2014)

Se metabolism pathways (scheme).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4066853&req=5

fig2: Se metabolism pathways (scheme).
Mentions: Although it is known that absorbed Se is incorporated into selenocysteine, selenomethionine, Se-methylselenocysteine, and several unidentified selenocompounds [29], mechanisms of its uptake, translocation, accumulation, and metabolism depend on the species [30]. According to Ip [27] and Lenz and Lens [2], water soluble selenites and selenates could be introduced into two metabolic pathways: (i) methylation directly or indirectly (through incorporation into selenomethionine) to methylselenol, dimethyl selenide, and trimethylselenonium ion which are vaporized or excreted and (ii) reduction to insoluble Se0, which could be either reoxidized to selenites, rarely selenates, or reduced to H2Se. H2Se, a precursor for selenoprotein synthesis, in the reaction with metal cations forms metal-Se that further either precipitates or oxidizes to selenites (Figure 2).

Bottom Line: The aim of this study was to evaluate the effect of high selenium (Se) concentrations on morphophysiological and ultrastructural properties of Pleurotus ostreatus.Biomass yield ranged between 11.8 g L(-1), in the control, and 6.8 g L(-1), at an Se concentration of 100.0 mg L(-1), while no production was detected at a concentration of 500.0 mg L(-1).Se content in the mycelia reached a peak (938.9 μg g(-1)) after cultivation in the medium enriched with Se at the concentration of 20.0 mg L(-1), while the highest absorption level (53.25%) was found in the medium enriched with 5.0 mg L(-1) Se.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia.

ABSTRACT
The aim of this study was to evaluate the effect of high selenium (Se) concentrations on morphophysiological and ultrastructural properties of Pleurotus ostreatus. Mycelium growth was good in media enriched with 5.0, 10.0, and 20.0 mg L(-1) of Se, concentration of 500.0 mg L(-1) strongly inhibited growth, and 1000.0 mg L(-1) was the minimum inhibitory concentration. Contrary to thin-walled, hyaline, branched, and anastomized hyphae with clamp-connections in the control, at Se concentrations of 100.0 and 500.0 mg L(-1), they were noticeably short, frequently septed and branched, with a more intensive extracellular matrix, and without clamp-connections. At high Se concentrations, hyphae with intact membrane, without cellular contents, with a high level of vacuolization, and with numerous proteinaceous bodies were observed. Biomass yield ranged between 11.8 g L(-1), in the control, and 6.8 g L(-1), at an Se concentration of 100.0 mg L(-1), while no production was detected at a concentration of 500.0 mg L(-1). Se content in the mycelia reached a peak (938.9 μg g(-1)) after cultivation in the medium enriched with Se at the concentration of 20.0 mg L(-1), while the highest absorption level (53.25%) was found in the medium enriched with 5.0 mg L(-1) Se.

Show MeSH