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Hydrolytic function of Exo1 in mammalian mismatch repair.

Shao H, Baitinger C, Soderblom EJ, Burdett V, Modrich P - Nucleic Acids Res. (2014)

Bottom Line: However, recent analysis of an Exo1-E109K knockin mouse has concluded that Exo1 function in mammalian mismatch repair is restricted to a structural role, a conclusion based on a prior report that N-terminal His-tagged Exo1-E109K is hydrolytically defective.Because Glu-109 is distant from the nuclease hydrolytic center, we have compared the activity of untagged full-length Exo1-E109K with that of wild type Exo1 and the hydrolytically defective active site mutant Exo1-D173A.We also consider the other phenotypes of the Exo1-E109K mouse in the context of Exo1 hydrolytic function.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

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Exo1 hydrolytic action is required for reconstituted mismatch repair. Mismatch repair of 5′- (panel A) or 3′- (panel B) G-T heteroduplex DNAs was determined in the presence of MutLα, RPA, RFC, PCNA and DNA polymerase δ in the absence or presence of MutSα, Exo1-D173A, Exo1-E109K or wild type Exo1 as indicated. Mismatch repair was determined as in Figure 3.
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Figure 5: Exo1 hydrolytic action is required for reconstituted mismatch repair. Mismatch repair of 5′- (panel A) or 3′- (panel B) G-T heteroduplex DNAs was determined in the presence of MutLα, RPA, RFC, PCNA and DNA polymerase δ in the absence or presence of MutSα, Exo1-D173A, Exo1-E109K or wild type Exo1 as indicated. Mismatch repair was determined as in Figure 3.

Mentions: Supplementation of MutSα, MutLα, Exo1, RPA, PCNA and RFC, the components required for bidirectional excision, with DNA polymerase δ and the dNTPs yields a minimal system that supports both 5′- and 3′-directed mismatch repair (37). As shown in Figure 5, wild type Exo1 and Exo1-E109K support MutSα-dependent mismatch correction on 5′- (panel A, compare lanes 4 and 5 with 8 and 9) or 3′-heteroduplex DNA (panel B, compare lanes 6 and 7, and lanes 8 and 9). However, as observed in cell extracts, hydrolytically deficient Exo1-D173A is incapable of supporting the repair of either heteroduplex in this purified system (panel A, lane 3; panel B, lane 4).


Hydrolytic function of Exo1 in mammalian mismatch repair.

Shao H, Baitinger C, Soderblom EJ, Burdett V, Modrich P - Nucleic Acids Res. (2014)

Exo1 hydrolytic action is required for reconstituted mismatch repair. Mismatch repair of 5′- (panel A) or 3′- (panel B) G-T heteroduplex DNAs was determined in the presence of MutLα, RPA, RFC, PCNA and DNA polymerase δ in the absence or presence of MutSα, Exo1-D173A, Exo1-E109K or wild type Exo1 as indicated. Mismatch repair was determined as in Figure 3.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4066806&req=5

Figure 5: Exo1 hydrolytic action is required for reconstituted mismatch repair. Mismatch repair of 5′- (panel A) or 3′- (panel B) G-T heteroduplex DNAs was determined in the presence of MutLα, RPA, RFC, PCNA and DNA polymerase δ in the absence or presence of MutSα, Exo1-D173A, Exo1-E109K or wild type Exo1 as indicated. Mismatch repair was determined as in Figure 3.
Mentions: Supplementation of MutSα, MutLα, Exo1, RPA, PCNA and RFC, the components required for bidirectional excision, with DNA polymerase δ and the dNTPs yields a minimal system that supports both 5′- and 3′-directed mismatch repair (37). As shown in Figure 5, wild type Exo1 and Exo1-E109K support MutSα-dependent mismatch correction on 5′- (panel A, compare lanes 4 and 5 with 8 and 9) or 3′-heteroduplex DNA (panel B, compare lanes 6 and 7, and lanes 8 and 9). However, as observed in cell extracts, hydrolytically deficient Exo1-D173A is incapable of supporting the repair of either heteroduplex in this purified system (panel A, lane 3; panel B, lane 4).

Bottom Line: However, recent analysis of an Exo1-E109K knockin mouse has concluded that Exo1 function in mammalian mismatch repair is restricted to a structural role, a conclusion based on a prior report that N-terminal His-tagged Exo1-E109K is hydrolytically defective.Because Glu-109 is distant from the nuclease hydrolytic center, we have compared the activity of untagged full-length Exo1-E109K with that of wild type Exo1 and the hydrolytically defective active site mutant Exo1-D173A.We also consider the other phenotypes of the Exo1-E109K mouse in the context of Exo1 hydrolytic function.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

Show MeSH
Related in: MedlinePlus