Protein kinase C controls activation of the DNA integrity checkpoint.
Bottom Line: This modification is a phosphorylation event mediated by Tel1.The expression of different mammalian PKC isoforms at the endogenous level in yeast pkc1 mutant cells revealed that PKCδ is able to activate the DNA integrity checkpoint.Our results indicate that the control of the DNA integrity checkpoint by PKC is a mechanism conserved from yeast to humans.
Affiliation: Departament de Bioquímica i Biologia Molecular. Universitat de València, 46100 Burjassot (Valencia), Spain.Show MeSH
Related in: MedlinePlus
Mentions: Next, we tested whether Pkc1 kinase activity is required for proper checkpoint activation in response to DNA damage. Rad53 activation was checked in pkc1 mutant cells expressing a kinase-dead mutant protein, Pkc1K853R (49). Whereas the expression of the wild-type PKC1 gene was able to sustain Rad53 activation, no Rad53 phosphorylation was observed in the cells expressing the Pkc1K853R mutant protein (Figure 3). This indicates that the kinase activity of Pkc1 is required for checkpoint control in response to DNA damage, suggesting that Pkc1 may act by phosphorylating essential targets for Rad53 activation.
Affiliation: Departament de Bioquímica i Biologia Molecular. Universitat de València, 46100 Burjassot (Valencia), Spain.