Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells.
Bottom Line: Exosomal miR-122, expressed and released by Huh7 cells and taken by miR-122 deficient HepG2 cells, was found to be effective in repression of target mRNAs and to reduce growth and proliferation of recipient HepG2 cells.Interestingly, in a reciprocal process, HepG2 secretes Insulin-like Growth Factor 1 (IGF1) that decreases miR-122 expression in Huh7 cells.This interaction is mediated via intercellular exosome-mediated miR-122 transfer and countered by a reciprocal IGF1-dependent anti-miR-122 signal.
Affiliation: RNA Biology Research Laboratory, Molecular and Human Genetics Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India.Show MeSH
Related in: MedlinePlus
Mentions: For the experiment in Figure 1J, the supernatant from Huh7 cells was centrifuged to clear cellular debris and this constituted the Huh7 CM. This was then further centrifuged in a centricon with a molecular weight cutoff of 100 kDa such that constituents having molecular weight of <100 kDa were filtered out and those which were >100 kDa were retained. The latter was then filter sterilized and added to HepG2 cells.
Affiliation: RNA Biology Research Laboratory, Molecular and Human Genetics Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India.