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Experimental protection of diabetic mice against Lethal P. aeruginosa infection by bacteriophage.

Shivshetty N, Hosamani R, Ahmed L, Oli AK, Sannauallah S, Sharanbassappa S, Patil SA, Kelmani CR - Biomed Res Int (2014)

Bottom Line: The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa.Evaluation of results confirmed that a single intraperitoneal injection of the phage dose (3 × 10(9) PFU/mL) was more effective than the multiple doses of imipenem.These results uphold the efficacy of phage therapy against pernicious P. aeruginosa infections, especially in cases of immunocompromised host.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.

ABSTRACT
The emergence of antibiotic-resistant bacterial strains has become a global crisis and is vulnerable for the exploration of alternative antibacterial therapies. The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa. P. aeruginosa was used to induce septicemia in streptozotocin (STZ) induced diabetic and nondiabetic mice by intraperitoneal (i.p.) injection of 3 × 10(8) CFU, resulting in a fatal bacteremia within 48 hrs. A single i.p. injection of 3 × 10(9) PFU phage GNCP showed efficient protection in both diabetic (90%) and nondiabetic (100%) bacteremic mice. It was further noted that the protection rate was reduced in diabetic mice when phage GNCP was administered after 4 h and 6 h of lethal bacterial challenge. In contrast, nondiabetic bacteremic mice were rescued even when treatment was delayed up to 20 h after lethal bacterial challenge. Evaluation of results confirmed that a single intraperitoneal injection of the phage dose (3 × 10(9) PFU/mL) was more effective than the multiple doses of imipenem. These results uphold the efficacy of phage therapy against pernicious P. aeruginosa infections, especially in cases of immunocompromised host.

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Histopathology of the liver. Sections of hematoxylin and eosin-stained liver (×500 magnification) are shown. (a) Diseased mice treated with phage GNCP. (b) Diseased mice treated with imipenem. (c) Diseased mice infected with lethal dose. (d) Control group of mice received PBS.
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fig7: Histopathology of the liver. Sections of hematoxylin and eosin-stained liver (×500 magnification) are shown. (a) Diseased mice treated with phage GNCP. (b) Diseased mice treated with imipenem. (c) Diseased mice infected with lethal dose. (d) Control group of mice received PBS.

Mentions: Histological examination of the group showed normal architecture with occasional dilated central vein with feathery degeneration of hepatocytes (Figure 7(d)). The section of diseased mice showed degeneration of hepatocytes with focal areas of hemorrhages (Figure 7(c)). The segment of the liver from the antibiotic treated mice showed eccentrically placed nuclei with vacuolated cytoplasm and few of hepatocytes showing feathery degeneration of hepatocytes with focal areas of hemorrhage (Figure 7(b)). The phage treated mice group showed the normal architecture with little quantity of regeneration of focal areas of hemorrhages (Figure 7(a)).


Experimental protection of diabetic mice against Lethal P. aeruginosa infection by bacteriophage.

Shivshetty N, Hosamani R, Ahmed L, Oli AK, Sannauallah S, Sharanbassappa S, Patil SA, Kelmani CR - Biomed Res Int (2014)

Histopathology of the liver. Sections of hematoxylin and eosin-stained liver (×500 magnification) are shown. (a) Diseased mice treated with phage GNCP. (b) Diseased mice treated with imipenem. (c) Diseased mice infected with lethal dose. (d) Control group of mice received PBS.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4066716&req=5

fig7: Histopathology of the liver. Sections of hematoxylin and eosin-stained liver (×500 magnification) are shown. (a) Diseased mice treated with phage GNCP. (b) Diseased mice treated with imipenem. (c) Diseased mice infected with lethal dose. (d) Control group of mice received PBS.
Mentions: Histological examination of the group showed normal architecture with occasional dilated central vein with feathery degeneration of hepatocytes (Figure 7(d)). The section of diseased mice showed degeneration of hepatocytes with focal areas of hemorrhages (Figure 7(c)). The segment of the liver from the antibiotic treated mice showed eccentrically placed nuclei with vacuolated cytoplasm and few of hepatocytes showing feathery degeneration of hepatocytes with focal areas of hemorrhage (Figure 7(b)). The phage treated mice group showed the normal architecture with little quantity of regeneration of focal areas of hemorrhages (Figure 7(a)).

Bottom Line: The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa.Evaluation of results confirmed that a single intraperitoneal injection of the phage dose (3 × 10(9) PFU/mL) was more effective than the multiple doses of imipenem.These results uphold the efficacy of phage therapy against pernicious P. aeruginosa infections, especially in cases of immunocompromised host.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka 585106, India.

ABSTRACT
The emergence of antibiotic-resistant bacterial strains has become a global crisis and is vulnerable for the exploration of alternative antibacterial therapies. The present study emphasizes the use of bacteriophage for the treatment of multidrug resistant P. aeruginosa. P. aeruginosa was used to induce septicemia in streptozotocin (STZ) induced diabetic and nondiabetic mice by intraperitoneal (i.p.) injection of 3 × 10(8) CFU, resulting in a fatal bacteremia within 48 hrs. A single i.p. injection of 3 × 10(9) PFU phage GNCP showed efficient protection in both diabetic (90%) and nondiabetic (100%) bacteremic mice. It was further noted that the protection rate was reduced in diabetic mice when phage GNCP was administered after 4 h and 6 h of lethal bacterial challenge. In contrast, nondiabetic bacteremic mice were rescued even when treatment was delayed up to 20 h after lethal bacterial challenge. Evaluation of results confirmed that a single intraperitoneal injection of the phage dose (3 × 10(9) PFU/mL) was more effective than the multiple doses of imipenem. These results uphold the efficacy of phage therapy against pernicious P. aeruginosa infections, especially in cases of immunocompromised host.

Show MeSH
Related in: MedlinePlus