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Erythrophagocytosis in Entamoeba histolytica and Entamoeba dispar: a comparative study.

Talamás-Lara D, Chávez-Munguía B, González-Robles A, Talamás-Rohana P, Salazar-Villatoro L, Durán-Díaz Á, Martínez-Palomo A - Biomed Res Int (2014)

Bottom Line: In the present study, we confirmed that E. dispar has lower erythrophagocytic capacity.Moreover, E. dispar showed a lower capacity to produce ROS compared with the invasive species and also showed a large population of amoebae that did not engulf any erythrocyte over time.Our results demonstrate that E. histolytica has a higher phagocytic capacity than E. dispar, including a higher rate of production of ROS in the course of ingesting red blood cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.

ABSTRACT
Entamoeba histolytica is the causative agent of human intestinal and liver amebiasis. The extraordinary phagocytic activity of E. histolytica trophozoites has been accepted as one of the virulence mechanisms responsible for their invasive capacity. The recognition of the noninvasive Entamoeba dispar as a different species has raised the question as to whether the lack of pathogenic potential of this ameba correlates with a limited phagocytic capacity. We have therefore compared the process of erythrophagocytosis in both species by means of light and video microscopy, hemoglobin measurement, and the estimation of reactive oxygen species (ROS). In the present study, we confirmed that E. dispar has lower erythrophagocytic capacity. We also observed by video microscopy a new event of erythrocyte opsonization-like in both species, being more characteristic in E. histolytica. Moreover, E. dispar showed a lower capacity to produce ROS compared with the invasive species and also showed a large population of amoebae that did not engulf any erythrocyte over time. Our results demonstrate that E. histolytica has a higher phagocytic capacity than E. dispar, including a higher rate of production of ROS in the course of ingesting red blood cells.

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Related in: MedlinePlus

Scatter diagrams showing correlations between area and the number of ingested erythrocytes with respect to interaction time. There is a direct and significant relation (P < 0.001) for both strains, meaning that the larger is the area of the amoeba, these engulf more erythrocytes. This ratio increases as time passes. The same trend for both strains was observed; however, correlations were higher for E. histolytica (“r” value).
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fig3: Scatter diagrams showing correlations between area and the number of ingested erythrocytes with respect to interaction time. There is a direct and significant relation (P < 0.001) for both strains, meaning that the larger is the area of the amoeba, these engulf more erythrocytes. This ratio increases as time passes. The same trend for both strains was observed; however, correlations were higher for E. histolytica (“r” value).

Mentions: To analyze whether the amoeba area could be a factor related with a higher rate of erythrophagocytosis, a dispersion analysis was performed. Results showed a relative, time-dependent association between amoeba area and number of phagocytosed erythrocytes, with “r” values of 0.5, 0.60, and 0.63 for E. dispar at 5, 10, and 15 min respectively, and with “r” values of 0.56, 0.59, and 0.70 for E. histolytica at 5, 10, and 15 min, respectively. Therefore, there was a direct relationship between the average surface area of the amoeba and the number of ingested red cells, with a significant difference (P < 0.001) between the two species; furthermore, this ratio increased with time. Although the same trend is observed for both species, the correlation values of E. histolytica were higher (Figure 3).


Erythrophagocytosis in Entamoeba histolytica and Entamoeba dispar: a comparative study.

Talamás-Lara D, Chávez-Munguía B, González-Robles A, Talamás-Rohana P, Salazar-Villatoro L, Durán-Díaz Á, Martínez-Palomo A - Biomed Res Int (2014)

Scatter diagrams showing correlations between area and the number of ingested erythrocytes with respect to interaction time. There is a direct and significant relation (P < 0.001) for both strains, meaning that the larger is the area of the amoeba, these engulf more erythrocytes. This ratio increases as time passes. The same trend for both strains was observed; however, correlations were higher for E. histolytica (“r” value).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4066688&req=5

fig3: Scatter diagrams showing correlations between area and the number of ingested erythrocytes with respect to interaction time. There is a direct and significant relation (P < 0.001) for both strains, meaning that the larger is the area of the amoeba, these engulf more erythrocytes. This ratio increases as time passes. The same trend for both strains was observed; however, correlations were higher for E. histolytica (“r” value).
Mentions: To analyze whether the amoeba area could be a factor related with a higher rate of erythrophagocytosis, a dispersion analysis was performed. Results showed a relative, time-dependent association between amoeba area and number of phagocytosed erythrocytes, with “r” values of 0.5, 0.60, and 0.63 for E. dispar at 5, 10, and 15 min respectively, and with “r” values of 0.56, 0.59, and 0.70 for E. histolytica at 5, 10, and 15 min, respectively. Therefore, there was a direct relationship between the average surface area of the amoeba and the number of ingested red cells, with a significant difference (P < 0.001) between the two species; furthermore, this ratio increased with time. Although the same trend is observed for both species, the correlation values of E. histolytica were higher (Figure 3).

Bottom Line: In the present study, we confirmed that E. dispar has lower erythrophagocytic capacity.Moreover, E. dispar showed a lower capacity to produce ROS compared with the invasive species and also showed a large population of amoebae that did not engulf any erythrocyte over time.Our results demonstrate that E. histolytica has a higher phagocytic capacity than E. dispar, including a higher rate of production of ROS in the course of ingesting red blood cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, IPN, Avenida Instituto Politécnico Nacional No. 2508, Colonia San Pedro Zacatenco, Delegación Gustavo A. Madero, 07360 Mexico City, DF, Mexico.

ABSTRACT
Entamoeba histolytica is the causative agent of human intestinal and liver amebiasis. The extraordinary phagocytic activity of E. histolytica trophozoites has been accepted as one of the virulence mechanisms responsible for their invasive capacity. The recognition of the noninvasive Entamoeba dispar as a different species has raised the question as to whether the lack of pathogenic potential of this ameba correlates with a limited phagocytic capacity. We have therefore compared the process of erythrophagocytosis in both species by means of light and video microscopy, hemoglobin measurement, and the estimation of reactive oxygen species (ROS). In the present study, we confirmed that E. dispar has lower erythrophagocytic capacity. We also observed by video microscopy a new event of erythrocyte opsonization-like in both species, being more characteristic in E. histolytica. Moreover, E. dispar showed a lower capacity to produce ROS compared with the invasive species and also showed a large population of amoebae that did not engulf any erythrocyte over time. Our results demonstrate that E. histolytica has a higher phagocytic capacity than E. dispar, including a higher rate of production of ROS in the course of ingesting red blood cells.

Show MeSH
Related in: MedlinePlus