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Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast.

Zanders SE, Eickbush MT, Yu JS, Kang JW, Fowler KR, Smith GR, Malik HS - Elife (2014)

Bottom Line: Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species.Two of these driving loci are linked by a chromosomal translocation and thus constitute a novel type of paired meiotic drive complex.Our study reveals how quickly multiple barriers to fertility can arise.

View Article: PubMed Central - PubMed

Affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, United States.

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Summary of meiotic phenotypes for Sk/Sp hybrids and diploids with one heterozygous chromosome and the distribution of alleles in their viable progeny.Detailed data underlying the plots in Figure 8. We induced Sk/Sp hybrids as well as diploids with only one heterozygous chromosome to undergo meiosis and measured their fertility using a viable spore yeild assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromosome 2 (his5) and chromosome 3 (ade6). Sk chromosomes in the crosses to generate the diploids (top) are shown as ‘K’s whereas Sp chromosomes are shown as ‘P’s. The exact genotypes of the strains containing ‘R’ chromosomes can be found in Figure 8—figure supplement 1. We genotyped the viable spore progeny of each diploid. The alleles shown in bold were contributed by the pure Sk (KKK) parent. We used co-dominant markers on chromosomes 2 and 3 to determine the number of chromosome 3 aneuploids and diploids amongst the viable spore progeny. Some of the PPPxKKK data are also shown in Figure 5, Figure 6B, and Figure 6—figure supplement 1.DOI:http://dx.doi.org/10.7554/eLife.02630.032
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fig8s2: Summary of meiotic phenotypes for Sk/Sp hybrids and diploids with one heterozygous chromosome and the distribution of alleles in their viable progeny.Detailed data underlying the plots in Figure 8. We induced Sk/Sp hybrids as well as diploids with only one heterozygous chromosome to undergo meiosis and measured their fertility using a viable spore yeild assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromosome 2 (his5) and chromosome 3 (ade6). Sk chromosomes in the crosses to generate the diploids (top) are shown as ‘K’s whereas Sp chromosomes are shown as ‘P’s. The exact genotypes of the strains containing ‘R’ chromosomes can be found in Figure 8—figure supplement 1. We genotyped the viable spore progeny of each diploid. The alleles shown in bold were contributed by the pure Sk (KKK) parent. We used co-dominant markers on chromosomes 2 and 3 to determine the number of chromosome 3 aneuploids and diploids amongst the viable spore progeny. Some of the PPPxKKK data are also shown in Figure 5, Figure 6B, and Figure 6—figure supplement 1.DOI:http://dx.doi.org/10.7554/eLife.02630.032

Mentions: We induced Sk/Sp hybrids as well as Sk/Sk and Sp/Sp control diploids to undergo meiosis and measured their fertility using a viable spore yield assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromsome 2 (his5) and chromsome 3 (ade6). We genotyped the viable spores from each diploid using these markers. In the rec12+Sk/Sp hybrids, the Sk parent contributed the his5+, lys1−, and ade6− alleles. In cases where the diploids had co-dominant markers on chromosomes 2 and 3, we were also able to determine the number of chromosome 3 aneuploids and diploids amongst the viable spores. Parts of these data are also presented in Figure 1H, Figure 3, Figure 5, Figure 6, Figure 8, and Figure 8—figure supplement 2.


Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast.

Zanders SE, Eickbush MT, Yu JS, Kang JW, Fowler KR, Smith GR, Malik HS - Elife (2014)

Summary of meiotic phenotypes for Sk/Sp hybrids and diploids with one heterozygous chromosome and the distribution of alleles in their viable progeny.Detailed data underlying the plots in Figure 8. We induced Sk/Sp hybrids as well as diploids with only one heterozygous chromosome to undergo meiosis and measured their fertility using a viable spore yeild assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromosome 2 (his5) and chromosome 3 (ade6). Sk chromosomes in the crosses to generate the diploids (top) are shown as ‘K’s whereas Sp chromosomes are shown as ‘P’s. The exact genotypes of the strains containing ‘R’ chromosomes can be found in Figure 8—figure supplement 1. We genotyped the viable spore progeny of each diploid. The alleles shown in bold were contributed by the pure Sk (KKK) parent. We used co-dominant markers on chromosomes 2 and 3 to determine the number of chromosome 3 aneuploids and diploids amongst the viable spore progeny. Some of the PPPxKKK data are also shown in Figure 5, Figure 6B, and Figure 6—figure supplement 1.DOI:http://dx.doi.org/10.7554/eLife.02630.032
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4066438&req=5

fig8s2: Summary of meiotic phenotypes for Sk/Sp hybrids and diploids with one heterozygous chromosome and the distribution of alleles in their viable progeny.Detailed data underlying the plots in Figure 8. We induced Sk/Sp hybrids as well as diploids with only one heterozygous chromosome to undergo meiosis and measured their fertility using a viable spore yeild assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromosome 2 (his5) and chromosome 3 (ade6). Sk chromosomes in the crosses to generate the diploids (top) are shown as ‘K’s whereas Sp chromosomes are shown as ‘P’s. The exact genotypes of the strains containing ‘R’ chromosomes can be found in Figure 8—figure supplement 1. We genotyped the viable spore progeny of each diploid. The alleles shown in bold were contributed by the pure Sk (KKK) parent. We used co-dominant markers on chromosomes 2 and 3 to determine the number of chromosome 3 aneuploids and diploids amongst the viable spore progeny. Some of the PPPxKKK data are also shown in Figure 5, Figure 6B, and Figure 6—figure supplement 1.DOI:http://dx.doi.org/10.7554/eLife.02630.032
Mentions: We induced Sk/Sp hybrids as well as Sk/Sk and Sp/Sp control diploids to undergo meiosis and measured their fertility using a viable spore yield assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromsome 2 (his5) and chromsome 3 (ade6). We genotyped the viable spores from each diploid using these markers. In the rec12+Sk/Sp hybrids, the Sk parent contributed the his5+, lys1−, and ade6− alleles. In cases where the diploids had co-dominant markers on chromosomes 2 and 3, we were also able to determine the number of chromosome 3 aneuploids and diploids amongst the viable spores. Parts of these data are also presented in Figure 1H, Figure 3, Figure 5, Figure 6, Figure 8, and Figure 8—figure supplement 2.

Bottom Line: Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species.Two of these driving loci are linked by a chromosomal translocation and thus constitute a novel type of paired meiotic drive complex.Our study reveals how quickly multiple barriers to fertility can arise.

View Article: PubMed Central - PubMed

Affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, United States.

Show MeSH
Related in: MedlinePlus