Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast.
Bottom Line: Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species.Two of these driving loci are linked by a chromosomal translocation and thus constitute a novel type of paired meiotic drive complex.Our study reveals how quickly multiple barriers to fertility can arise.
Affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, United States.Show MeSH
Related in: MedlinePlus
Mentions: We induced Sk/Sp hybrids as well as Sk/Sk and Sp/Sp control diploids to undergo meiosis and measured their fertility using a viable spore yield assay (VSY). All diploids were heterozygous for genetic markers on chromosome 1 (lys1), chromsome 2 (his5) and chromsome 3 (ade6). We genotyped the viable spores from each diploid using these markers. In the rec12+Sk/Sp hybrids, the Sk parent contributed the his5+, lys1−, and ade6− alleles. In cases where the diploids had co-dominant markers on chromosomes 2 and 3, we were also able to determine the number of chromosome 3 aneuploids and diploids amongst the viable spores. Parts of these data are also presented in Figure 1H, Figure 3, Figure 5, Figure 6, Figure 8, and Figure 8—figure supplement 2.
Affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, United States.