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Phosphatidic acid enhances mTOR signaling and resistance exercise induced hypertrophy.

Joy JM, Gundermann DM, Lowery RP, Jäger R, McCleary SA, Purpura M, Roberts MD, Wilson SM, Hornberger TA, Wilson JM - Nutr Metab (Lond) (2014)

Bottom Line: Therefore, the purpose of this study was to compare the effects of various PA precursors and phospholipids on their ability to stimulate mTOR signaling and its ability to augment resistance training-induced changes in body composition and performance.In phase one, soy-phosphatidylserine, soy-Lyso-PA, egg-PA, and soy-PA stimulated mTOR signaling, and the effects of soy-PA (+636%) were significantly greater than egg-PA (+221%).In phase two, PA significantly increased lean body mass (+2.4 kg), cross sectional area (+1.0 cm), and leg press strength (+51.9 kg) over placebo.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Health Sciences and Human Performance, The University of Tampa, 401 W. Kennedy Blvd., Box 30 F, Tampa, FL 33606, USA.

ABSTRACT

Introduction: The lipid messenger phosphatidic acid (PA) plays a critical role in the stimulation of mTOR signaling. However, the mechanism by which PA stimulates mTOR is currently unknown. Therefore, the purpose of this study was to compare the effects of various PA precursors and phospholipids on their ability to stimulate mTOR signaling and its ability to augment resistance training-induced changes in body composition and performance.

Methods: In phase one, C2C12 myoblasts cells were stimulated with different phospholipids and phospholipid precursors derived from soy and egg sources. The ratio of phosphorylated p70 (P-p70-389) to total p70 was then used as readout for mTOR signaling. In phase two, resistance trained subjects (n = 28, 21 ± 3 years, 77 ± 4 kg, 176 ± 9 cm) consumed either 750 mg PA daily or placebo and each took part in an 8 week periodized resistance training program.

Results: In phase one, soy-phosphatidylserine, soy-Lyso-PA, egg-PA, and soy-PA stimulated mTOR signaling, and the effects of soy-PA (+636%) were significantly greater than egg-PA (+221%). In phase two, PA significantly increased lean body mass (+2.4 kg), cross sectional area (+1.0 cm), and leg press strength (+51.9 kg) over placebo.

Conclusion: PA significantly activates mTOR and significantly improved responses in skeletal muscle hypertrophy, lean body mass, and maximal strength to resistance exercise.

No MeSH data available.


Related in: MedlinePlus

Changes in body composition. a. PA significantly improves the resistance training-induced increase in CSA following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). b. PA significantly improves the resistance training-induced increase in LBM following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). c. PA and PLA both experience significant fat loss following 8 weeks of periodized resistance training. However, only a trend is observed between groups over time. (*denotes significantly different from pre).
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Figure 2: Changes in body composition. a. PA significantly improves the resistance training-induced increase in CSA following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). b. PA significantly improves the resistance training-induced increase in LBM following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). c. PA and PLA both experience significant fat loss following 8 weeks of periodized resistance training. However, only a trend is observed between groups over time. (*denotes significantly different from pre).

Mentions: No differences existed between groups at baseline for any measure. There was a significant group x time effect (p = 0.02) for CSA (Figure 2a), in which the PA group increased (pre 4.5 ± 1.1 cm2, post 5.5 ± 1.3 cm2, Effect Size (ES) = 0.92) to a greater extent than the PLA group (pre 4.5 ± 1.1 cm2, post 5.1 ± 1.2 cm2, ES = 0.52). There was a significant group × time effect (p = 0.01) for LBM (Figure 2b), in which the PA group increased to a greater extent (pre 59.7 ± 6.0 kg, post 62.1 ± 5.5 kg, ES = 0.42) than the PLA group (pre 59.5 ± 4.7 kg, post 60.7 ± 4.7 kg, ES = 0.26). There was a significant time effect (p = 0.02) for Total Body Mass (TBM) in which the PA group increased from 78.1 ± 8.7 to 78.7 ± 7.9 kg and the PLA group increased from 75.7 ± 5.8 to 76.5 ± 6.1 kg, but no differences existed between groups (p = 0.71). There was a significant time effect (p < 0.01) for fat mass (Figure 2c), in which there was a trend (p = 0.068) for fat mass to decrease to a greater extent in the PA group (pre 15.1 ± 4.8 kg, post 13.8 ± 4.2 kg, ES = −0.28) than the PLA group (pre 13.0 ± 6.5 kg, post 12.5 ± 6.9 kg, ES = −0.07).


Phosphatidic acid enhances mTOR signaling and resistance exercise induced hypertrophy.

Joy JM, Gundermann DM, Lowery RP, Jäger R, McCleary SA, Purpura M, Roberts MD, Wilson SM, Hornberger TA, Wilson JM - Nutr Metab (Lond) (2014)

Changes in body composition. a. PA significantly improves the resistance training-induced increase in CSA following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). b. PA significantly improves the resistance training-induced increase in LBM following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). c. PA and PLA both experience significant fat loss following 8 weeks of periodized resistance training. However, only a trend is observed between groups over time. (*denotes significantly different from pre).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4066292&req=5

Figure 2: Changes in body composition. a. PA significantly improves the resistance training-induced increase in CSA following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). b. PA significantly improves the resistance training-induced increase in LBM following 8 weeks of periodized resistance training. Data presented are means and standard deviations. (*denotes significantly different from pre, # denotes significantly different from placebo). c. PA and PLA both experience significant fat loss following 8 weeks of periodized resistance training. However, only a trend is observed between groups over time. (*denotes significantly different from pre).
Mentions: No differences existed between groups at baseline for any measure. There was a significant group x time effect (p = 0.02) for CSA (Figure 2a), in which the PA group increased (pre 4.5 ± 1.1 cm2, post 5.5 ± 1.3 cm2, Effect Size (ES) = 0.92) to a greater extent than the PLA group (pre 4.5 ± 1.1 cm2, post 5.1 ± 1.2 cm2, ES = 0.52). There was a significant group × time effect (p = 0.01) for LBM (Figure 2b), in which the PA group increased to a greater extent (pre 59.7 ± 6.0 kg, post 62.1 ± 5.5 kg, ES = 0.42) than the PLA group (pre 59.5 ± 4.7 kg, post 60.7 ± 4.7 kg, ES = 0.26). There was a significant time effect (p = 0.02) for Total Body Mass (TBM) in which the PA group increased from 78.1 ± 8.7 to 78.7 ± 7.9 kg and the PLA group increased from 75.7 ± 5.8 to 76.5 ± 6.1 kg, but no differences existed between groups (p = 0.71). There was a significant time effect (p < 0.01) for fat mass (Figure 2c), in which there was a trend (p = 0.068) for fat mass to decrease to a greater extent in the PA group (pre 15.1 ± 4.8 kg, post 13.8 ± 4.2 kg, ES = −0.28) than the PLA group (pre 13.0 ± 6.5 kg, post 12.5 ± 6.9 kg, ES = −0.07).

Bottom Line: Therefore, the purpose of this study was to compare the effects of various PA precursors and phospholipids on their ability to stimulate mTOR signaling and its ability to augment resistance training-induced changes in body composition and performance.In phase one, soy-phosphatidylserine, soy-Lyso-PA, egg-PA, and soy-PA stimulated mTOR signaling, and the effects of soy-PA (+636%) were significantly greater than egg-PA (+221%).In phase two, PA significantly increased lean body mass (+2.4 kg), cross sectional area (+1.0 cm), and leg press strength (+51.9 kg) over placebo.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Health Sciences and Human Performance, The University of Tampa, 401 W. Kennedy Blvd., Box 30 F, Tampa, FL 33606, USA.

ABSTRACT

Introduction: The lipid messenger phosphatidic acid (PA) plays a critical role in the stimulation of mTOR signaling. However, the mechanism by which PA stimulates mTOR is currently unknown. Therefore, the purpose of this study was to compare the effects of various PA precursors and phospholipids on their ability to stimulate mTOR signaling and its ability to augment resistance training-induced changes in body composition and performance.

Methods: In phase one, C2C12 myoblasts cells were stimulated with different phospholipids and phospholipid precursors derived from soy and egg sources. The ratio of phosphorylated p70 (P-p70-389) to total p70 was then used as readout for mTOR signaling. In phase two, resistance trained subjects (n = 28, 21 ± 3 years, 77 ± 4 kg, 176 ± 9 cm) consumed either 750 mg PA daily or placebo and each took part in an 8 week periodized resistance training program.

Results: In phase one, soy-phosphatidylserine, soy-Lyso-PA, egg-PA, and soy-PA stimulated mTOR signaling, and the effects of soy-PA (+636%) were significantly greater than egg-PA (+221%). In phase two, PA significantly increased lean body mass (+2.4 kg), cross sectional area (+1.0 cm), and leg press strength (+51.9 kg) over placebo.

Conclusion: PA significantly activates mTOR and significantly improved responses in skeletal muscle hypertrophy, lean body mass, and maximal strength to resistance exercise.

No MeSH data available.


Related in: MedlinePlus