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Ethanol modulates spontaneous calcium waves in axonal growth cones in vitro.

Lindsley TA, Mazurkiewicz JE - Brain Sci (2013)

Bottom Line: Using time-series fluorescence calcium imaging we found that acute treatment of fetal rat hippocampal neurons with 43 or 87 mM ethanol at an early stage of development in culture decreased the percent of axon growth cones showing at least one Ca2+ wave during 10 min of recording, from 18% in controls to 5% in cultures exposed to ethanol.As expected, waves were most prevalent in stationary or retracting growth cones in all treatment groups, except in cultures exposed chronically to 87 mM ethanol.Thus, the relationship between growth cone Ca2+ waves and axon growth dynamics is disrupted by ethanol.

View Article: PubMed Central - PubMed

Affiliation: Center for Neuropharmacology & Neuroscience, Albany Medical College (MC-136), 47 New Scotland Ave., Albany, NY 12208, USA. lindslt@mail.amc.edu.

ABSTRACT
In developing neurons the frequency of long duration, spontaneous, transient calcium (Ca2+) elevations localized to the growth cone, is inversely related to the rate of axon elongation and increases several fold when axons pause. Here we report that these spontaneous Ca2+ transients with slow kinetics, called Ca2+ waves, are modulated by conditions of ethanol exposure that alter axonal growth dynamics. Using time-series fluorescence calcium imaging we found that acute treatment of fetal rat hippocampal neurons with 43 or 87 mM ethanol at an early stage of development in culture decreased the percent of axon growth cones showing at least one Ca2+ wave during 10 min of recording, from 18% in controls to 5% in cultures exposed to ethanol. Chronic exposure to 43 mM ethanol also reduced the incidence of Ca2+ waves to 8%, but exposure to 87 mM ethanol increased their incidence to 31%. Neither chronic nor acute ethanol affected the peak amplitude, time to peak or total duration of Ca2+ waves. In some experiments, we determined the temporal correlation between Ca2+ waves and growth and non-growth phases of axonal growth dynamics. As expected, waves were most prevalent in stationary or retracting growth cones in all treatment groups, except in cultures exposed chronically to 87 mM ethanol. Thus, the relationship between growth cone Ca2+ waves and axon growth dynamics is disrupted by ethanol.

No MeSH data available.


Related in: MedlinePlus

Ethanol alters the incidence of spontaneous calcium waves in axonal growth cones. The plot shows the percent of neurons in each treatment group that exhibited at least one Ca2+ wave during the 10 min. recording. The data are combined from 39 separate cultures with a total of 453 growth cones analyzed (54–91 per treatment group). * p < 0.05, ** p < 0.001, chi square, compared to control group for acute or chronic treatment.
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brainsci-03-00615-f003: Ethanol alters the incidence of spontaneous calcium waves in axonal growth cones. The plot shows the percent of neurons in each treatment group that exhibited at least one Ca2+ wave during the 10 min. recording. The data are combined from 39 separate cultures with a total of 453 growth cones analyzed (54–91 per treatment group). * p < 0.05, ** p < 0.001, chi square, compared to control group for acute or chronic treatment.

Mentions: We, therefore, determined the effect of acute or chronic ethanol exposure on incidence of spontaneous Ca2+ waves in axonal growth cones of hippocampal neurons in dissociated cultures Figure 3 shows the proportion (%) of axonal growth cones in each treatment group that displayed at least 1 wave during a 10 min recording period. Chi square tests were performed to determine if the observed proportion of growth cones displaying waves after ethanol treatment was different than the expected proportion as indicated by proportion of control growth cones displaying waves. Compared to control cultures without ethanol, the incidence of spontaneous waves was decreased by acute or chronic exposure to 43 mM ethanol and by acute exposure to 87 mM ethanol. In contrast, chronic exposure to 87 mM ethanol increased the incidence of growth cones with waves.


Ethanol modulates spontaneous calcium waves in axonal growth cones in vitro.

Lindsley TA, Mazurkiewicz JE - Brain Sci (2013)

Ethanol alters the incidence of spontaneous calcium waves in axonal growth cones. The plot shows the percent of neurons in each treatment group that exhibited at least one Ca2+ wave during the 10 min. recording. The data are combined from 39 separate cultures with a total of 453 growth cones analyzed (54–91 per treatment group). * p < 0.05, ** p < 0.001, chi square, compared to control group for acute or chronic treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061854&req=5

brainsci-03-00615-f003: Ethanol alters the incidence of spontaneous calcium waves in axonal growth cones. The plot shows the percent of neurons in each treatment group that exhibited at least one Ca2+ wave during the 10 min. recording. The data are combined from 39 separate cultures with a total of 453 growth cones analyzed (54–91 per treatment group). * p < 0.05, ** p < 0.001, chi square, compared to control group for acute or chronic treatment.
Mentions: We, therefore, determined the effect of acute or chronic ethanol exposure on incidence of spontaneous Ca2+ waves in axonal growth cones of hippocampal neurons in dissociated cultures Figure 3 shows the proportion (%) of axonal growth cones in each treatment group that displayed at least 1 wave during a 10 min recording period. Chi square tests were performed to determine if the observed proportion of growth cones displaying waves after ethanol treatment was different than the expected proportion as indicated by proportion of control growth cones displaying waves. Compared to control cultures without ethanol, the incidence of spontaneous waves was decreased by acute or chronic exposure to 43 mM ethanol and by acute exposure to 87 mM ethanol. In contrast, chronic exposure to 87 mM ethanol increased the incidence of growth cones with waves.

Bottom Line: Using time-series fluorescence calcium imaging we found that acute treatment of fetal rat hippocampal neurons with 43 or 87 mM ethanol at an early stage of development in culture decreased the percent of axon growth cones showing at least one Ca2+ wave during 10 min of recording, from 18% in controls to 5% in cultures exposed to ethanol.As expected, waves were most prevalent in stationary or retracting growth cones in all treatment groups, except in cultures exposed chronically to 87 mM ethanol.Thus, the relationship between growth cone Ca2+ waves and axon growth dynamics is disrupted by ethanol.

View Article: PubMed Central - PubMed

Affiliation: Center for Neuropharmacology & Neuroscience, Albany Medical College (MC-136), 47 New Scotland Ave., Albany, NY 12208, USA. lindslt@mail.amc.edu.

ABSTRACT
In developing neurons the frequency of long duration, spontaneous, transient calcium (Ca2+) elevations localized to the growth cone, is inversely related to the rate of axon elongation and increases several fold when axons pause. Here we report that these spontaneous Ca2+ transients with slow kinetics, called Ca2+ waves, are modulated by conditions of ethanol exposure that alter axonal growth dynamics. Using time-series fluorescence calcium imaging we found that acute treatment of fetal rat hippocampal neurons with 43 or 87 mM ethanol at an early stage of development in culture decreased the percent of axon growth cones showing at least one Ca2+ wave during 10 min of recording, from 18% in controls to 5% in cultures exposed to ethanol. Chronic exposure to 43 mM ethanol also reduced the incidence of Ca2+ waves to 8%, but exposure to 87 mM ethanol increased their incidence to 31%. Neither chronic nor acute ethanol affected the peak amplitude, time to peak or total duration of Ca2+ waves. In some experiments, we determined the temporal correlation between Ca2+ waves and growth and non-growth phases of axonal growth dynamics. As expected, waves were most prevalent in stationary or retracting growth cones in all treatment groups, except in cultures exposed chronically to 87 mM ethanol. Thus, the relationship between growth cone Ca2+ waves and axon growth dynamics is disrupted by ethanol.

No MeSH data available.


Related in: MedlinePlus