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Cocaine-induced reinstatement of a conditioned place preference in developing rats: involvement of the d2 receptor.

Badanich KA, Kirstein CL - Brain Sci (2012)

Bottom Line: The present study determined whether the effects of intra-ventral tegmental area (VTA) or intra-nucleus accumbens septi (NAcc) dopamine (DA) D2 receptor antagonist infusions would block (or potentiate) cocaine-induced reinstatement of conditioned place preferences.Adolescent rats (postnatal day (PND 28-39)) were trained to express a cocaine place preference.These data suggest intrinsic compensatory mechanisms in the mesolimbic DA pathway mediate responsivity to cocaine-induced reinstatement of a conditioned place preference during development.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, College of Arts and Sciences, University of South Florida Sarasota-Manatee, 8350 N. Tamiami Trail, Sarasota, FL 34243, USA. badanich@sar.usf.edu.

ABSTRACT
Reinstatement of conditioned place preferences have been used to investigate physiological mechanisms mediating drug-seeking behavior in adolescent and adult rodents; however, it is still unclear how psychostimulant exposure during adolescence affects neuron communication and whether these changes would elicit enhanced drug-seeking behavior later in adulthood. The present study determined whether the effects of intra-ventral tegmental area (VTA) or intra-nucleus accumbens septi (NAcc) dopamine (DA) D2 receptor antagonist infusions would block (or potentiate) cocaine-induced reinstatement of conditioned place preferences. Adolescent rats (postnatal day (PND 28-39)) were trained to express a cocaine place preference. The involvement of D2 receptors on cocaine-induced reinstatement was determined by intra-VTA or intra-NAcc infusion of the DA D2 receptor antagonist sulpiride (100 μM) during a cocaine-primed reinstatement test (10 mg/kg cocaine, i.p.). Infusion of sulpiride into the VTA but not the NAcc blocked reinstatement of conditioned place preference. These data suggest intrinsic compensatory mechanisms in the mesolimbic DA pathway mediate responsivity to cocaine-induced reinstatement of a conditioned place preference during development.

No MeSH data available.


Related in: MedlinePlus

Place Conditioning. Cocaine place conditioning during adolescence. Cocaine treated adolescent rats showed a place preference. * differs from baseline. # differs from baseline and saline. Bars denote means and SEM. Sample sizes = 31 or 32 rats per group.
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brainsci-02-00573-f002: Place Conditioning. Cocaine place conditioning during adolescence. Cocaine treated adolescent rats showed a place preference. * differs from baseline. # differs from baseline and saline. Bars denote means and SEM. Sample sizes = 31 or 32 rats per group.

Mentions: It was the aim of the present study to determine whether cocaine-induced reinstatement of cocaine place conditioning was mediated by D2 receptors in the VTA and NAcc. Pretreatment (saline vs. cocaine) and Group (sal/sal; sal/sul; coc/sal; coc/sul) were between subjects variables and Session (baseline vs. expression) was the repeated measure. The dependent measure for place conditioning, extinction and reinstatement was time spent in the paired environment expressed as a difference score (second in paired environment − second in unpaired environment). A two-way analyses of variance (ANOVA) was used to analyze place conditioning scores (mixed model: Session (baseline vs. expression) × Pretreatment (saline vs. cocaine)). An unpaired t-test of Pretreatment effects was used to evaluate extinction scores. One way-between subjects ANOVA of Group were used to analyze reinstatement scores in VTA and NAcc infused rats. Simple effects (for Figure 2) and Fisher’s least significant difference (LSD) post hoc analyses (for Figure 2, Figure 3 and Figure 4) were used to isolate Session, Pretreatment, and Group effects. All statistical analyses were determined significant at the 0.05 alpha level.


Cocaine-induced reinstatement of a conditioned place preference in developing rats: involvement of the d2 receptor.

Badanich KA, Kirstein CL - Brain Sci (2012)

Place Conditioning. Cocaine place conditioning during adolescence. Cocaine treated adolescent rats showed a place preference. * differs from baseline. # differs from baseline and saline. Bars denote means and SEM. Sample sizes = 31 or 32 rats per group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061817&req=5

brainsci-02-00573-f002: Place Conditioning. Cocaine place conditioning during adolescence. Cocaine treated adolescent rats showed a place preference. * differs from baseline. # differs from baseline and saline. Bars denote means and SEM. Sample sizes = 31 or 32 rats per group.
Mentions: It was the aim of the present study to determine whether cocaine-induced reinstatement of cocaine place conditioning was mediated by D2 receptors in the VTA and NAcc. Pretreatment (saline vs. cocaine) and Group (sal/sal; sal/sul; coc/sal; coc/sul) were between subjects variables and Session (baseline vs. expression) was the repeated measure. The dependent measure for place conditioning, extinction and reinstatement was time spent in the paired environment expressed as a difference score (second in paired environment − second in unpaired environment). A two-way analyses of variance (ANOVA) was used to analyze place conditioning scores (mixed model: Session (baseline vs. expression) × Pretreatment (saline vs. cocaine)). An unpaired t-test of Pretreatment effects was used to evaluate extinction scores. One way-between subjects ANOVA of Group were used to analyze reinstatement scores in VTA and NAcc infused rats. Simple effects (for Figure 2) and Fisher’s least significant difference (LSD) post hoc analyses (for Figure 2, Figure 3 and Figure 4) were used to isolate Session, Pretreatment, and Group effects. All statistical analyses were determined significant at the 0.05 alpha level.

Bottom Line: The present study determined whether the effects of intra-ventral tegmental area (VTA) or intra-nucleus accumbens septi (NAcc) dopamine (DA) D2 receptor antagonist infusions would block (or potentiate) cocaine-induced reinstatement of conditioned place preferences.Adolescent rats (postnatal day (PND 28-39)) were trained to express a cocaine place preference.These data suggest intrinsic compensatory mechanisms in the mesolimbic DA pathway mediate responsivity to cocaine-induced reinstatement of a conditioned place preference during development.

View Article: PubMed Central - PubMed

Affiliation: Department of Psychology, College of Arts and Sciences, University of South Florida Sarasota-Manatee, 8350 N. Tamiami Trail, Sarasota, FL 34243, USA. badanich@sar.usf.edu.

ABSTRACT
Reinstatement of conditioned place preferences have been used to investigate physiological mechanisms mediating drug-seeking behavior in adolescent and adult rodents; however, it is still unclear how psychostimulant exposure during adolescence affects neuron communication and whether these changes would elicit enhanced drug-seeking behavior later in adulthood. The present study determined whether the effects of intra-ventral tegmental area (VTA) or intra-nucleus accumbens septi (NAcc) dopamine (DA) D2 receptor antagonist infusions would block (or potentiate) cocaine-induced reinstatement of conditioned place preferences. Adolescent rats (postnatal day (PND 28-39)) were trained to express a cocaine place preference. The involvement of D2 receptors on cocaine-induced reinstatement was determined by intra-VTA or intra-NAcc infusion of the DA D2 receptor antagonist sulpiride (100 μM) during a cocaine-primed reinstatement test (10 mg/kg cocaine, i.p.). Infusion of sulpiride into the VTA but not the NAcc blocked reinstatement of conditioned place preference. These data suggest intrinsic compensatory mechanisms in the mesolimbic DA pathway mediate responsivity to cocaine-induced reinstatement of a conditioned place preference during development.

No MeSH data available.


Related in: MedlinePlus