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Nicotine exposure increases the complexity of dopamine neurons in the parainterfascicular nucleus (PIF) sub-region of VTA.

Zhang D, Dragomir A, Akay YM, Akay M - J Neuroeng Rehabil (2014)

Bottom Line: Approximate entropy values were then grouped according to each anatomic location including the PBP, PIF and PN.Our results have shown that the local field potentials corresponding to the neurons located in the PIF region of the VTA have ApEn values significantly higher (p = 2x10-4) in the maternal nicotine cases when compared to the saline.Therefore, we speculate that the dopamine neurons located in the PIF sub-region of the VTA are very likely involved with the nicotine addiction.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Cullen College of Engineering, University of Houston, Houston, TX 77204, USA. makay@uh.edu.

ABSTRACT

Background: Recent publications highlight differences within the sub-regions of the ventral tegmental area (VTA) including the parabrachial pigmented nucleus (PBP), parainterfascicular nucleus (PIF) and paranigral nucleus (PN) in the projections to the prefrontal cortex (PFC) and the glutamatergic pathway.

Methods: In order to characterize the effects of prenatal nicotine exposure on the mesocorticolimbic system of the rat offspring, local field potentials were recorded from 27 sites across the VTA of 9 rats aged 40-55 days. The extracellular VTA neural activities were analyzed using Approximate Entropy (ApEn) method. Approximate entropy values were then grouped according to each anatomic location including the PBP, PIF and PN.

Results: Our results have shown that the local field potentials corresponding to the neurons located in the PIF region of the VTA have ApEn values significantly higher (p = 2x10-4) in the maternal nicotine cases when compared to the saline.

Conclusion: Therefore, we speculate that the dopamine neurons located in the PIF sub-region of the VTA are very likely involved with the nicotine addiction.

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Related in: MedlinePlus

Typical LFP traces recorded from VTA. This figure shows the typical LFP traces from the PBP, PIF and PN. Left panels (A) is of control group rats, and right panels (B) is of rats from the maternal nicotine exposure group.
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Figure 2: Typical LFP traces recorded from VTA. This figure shows the typical LFP traces from the PBP, PIF and PN. Left panels (A) is of control group rats, and right panels (B) is of rats from the maternal nicotine exposure group.

Mentions: A total of 9 offspring rats were used in the present study, 6 from dams exposed to nicotine during gestational period, while the remaining three were from dams which received the saline treatment, as described in the Methods section. Before analysis of the LFP recordings using the ApEn method, all data samples were denoised using the wavelet transform and detrended by removing their mean. Recordings were performed from several sites across the VTA, targeting three major sub-regions, namely the PBP, PIF and PN, as shown in Figure 1A and B. Generally we recorded ~3 min of LFP activity from each recording site. For each individual recording a number of six consecutive 10s long segments were chosen for ApEn analysis, after ~1 minute of stable recording was achieved. A total of 66 recordings were performed from 27 different sites within the VTA, 25 from offspring of saline treated dams (control group) and 41 from offspring of dams exposed to nicotine during gestational period (maternal nicotine exposure group).Figure 2 shows typical LFP traces recorded from the different VTA sub-regions of control group rats (left panels) and of rats from the maternal nicotine exposure group (right panels). The top panels display LFPs recorded from the PBP sub-region, the middle panels show LFP signals from the PIF sub-region, while the bottom panels display signals recorded from the PN sub-region and for all recordings within a specific VTA sub-region (PBP, PIF and PN) pertaining to the specific animal.


Nicotine exposure increases the complexity of dopamine neurons in the parainterfascicular nucleus (PIF) sub-region of VTA.

Zhang D, Dragomir A, Akay YM, Akay M - J Neuroeng Rehabil (2014)

Typical LFP traces recorded from VTA. This figure shows the typical LFP traces from the PBP, PIF and PN. Left panels (A) is of control group rats, and right panels (B) is of rats from the maternal nicotine exposure group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4061779&req=5

Figure 2: Typical LFP traces recorded from VTA. This figure shows the typical LFP traces from the PBP, PIF and PN. Left panels (A) is of control group rats, and right panels (B) is of rats from the maternal nicotine exposure group.
Mentions: A total of 9 offspring rats were used in the present study, 6 from dams exposed to nicotine during gestational period, while the remaining three were from dams which received the saline treatment, as described in the Methods section. Before analysis of the LFP recordings using the ApEn method, all data samples were denoised using the wavelet transform and detrended by removing their mean. Recordings were performed from several sites across the VTA, targeting three major sub-regions, namely the PBP, PIF and PN, as shown in Figure 1A and B. Generally we recorded ~3 min of LFP activity from each recording site. For each individual recording a number of six consecutive 10s long segments were chosen for ApEn analysis, after ~1 minute of stable recording was achieved. A total of 66 recordings were performed from 27 different sites within the VTA, 25 from offspring of saline treated dams (control group) and 41 from offspring of dams exposed to nicotine during gestational period (maternal nicotine exposure group).Figure 2 shows typical LFP traces recorded from the different VTA sub-regions of control group rats (left panels) and of rats from the maternal nicotine exposure group (right panels). The top panels display LFPs recorded from the PBP sub-region, the middle panels show LFP signals from the PIF sub-region, while the bottom panels display signals recorded from the PN sub-region and for all recordings within a specific VTA sub-region (PBP, PIF and PN) pertaining to the specific animal.

Bottom Line: Approximate entropy values were then grouped according to each anatomic location including the PBP, PIF and PN.Our results have shown that the local field potentials corresponding to the neurons located in the PIF region of the VTA have ApEn values significantly higher (p = 2x10-4) in the maternal nicotine cases when compared to the saline.Therefore, we speculate that the dopamine neurons located in the PIF sub-region of the VTA are very likely involved with the nicotine addiction.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Cullen College of Engineering, University of Houston, Houston, TX 77204, USA. makay@uh.edu.

ABSTRACT

Background: Recent publications highlight differences within the sub-regions of the ventral tegmental area (VTA) including the parabrachial pigmented nucleus (PBP), parainterfascicular nucleus (PIF) and paranigral nucleus (PN) in the projections to the prefrontal cortex (PFC) and the glutamatergic pathway.

Methods: In order to characterize the effects of prenatal nicotine exposure on the mesocorticolimbic system of the rat offspring, local field potentials were recorded from 27 sites across the VTA of 9 rats aged 40-55 days. The extracellular VTA neural activities were analyzed using Approximate Entropy (ApEn) method. Approximate entropy values were then grouped according to each anatomic location including the PBP, PIF and PN.

Results: Our results have shown that the local field potentials corresponding to the neurons located in the PIF region of the VTA have ApEn values significantly higher (p = 2x10-4) in the maternal nicotine cases when compared to the saline.

Conclusion: Therefore, we speculate that the dopamine neurons located in the PIF sub-region of the VTA are very likely involved with the nicotine addiction.

Show MeSH
Related in: MedlinePlus