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Rhodotorula mucilaginosa Keratitis: a rare fungus from Eastern India.

Saha S, Sengupta J, Chatterjee D, Banerjee D - Indian J Ophthalmol (2014)

Bottom Line: Molecular identification of the organism was performed which showed 100% homology with Rhodotorula mucilaginosa.Management of these cases is difficult often necessitating surgical procedures.However further reports are necessary to understand the disease and establish a treatment protocol.

View Article: PubMed Central - PubMed

Affiliation: Ocular Microbiology Laboratory Services, Priyamvada Birla Aravind Eye Hospital, 10 Loudon Street, Kolkata; Department of Microbiology, Vidyasagar University, Midnapore, India.

ABSTRACT
Rhodotorula mucilaginosa rarely cause keratitis in immunocompromised individuals. A 30 year old male with history of minor trauma presented with cotton wool like stromal infiltration and hypopyon in left eye. Microbiological examination of corneal scraping showed fungal hyphae and yeast cells in direct smear. Molecular identification of the organism was performed which showed 100% homology with Rhodotorula mucilaginosa. Management of these cases is difficult often necessitating surgical procedures. However further reports are necessary to understand the disease and establish a treatment protocol.

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Related in: MedlinePlus

Lactophenol cotton blue mount in 100X
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Figure 4: Lactophenol cotton blue mount in 100X

Mentions: After 12 days of incubation orange pigmented glistening yeast like colonies were grown on SDA and PDA [Fig. 3]. Unicellular blastoconidia and elongated globose forms were seen in Grams staining from culture. Lactophenol cotton blue mount from the colonies showed capsulated yeast cells without hyphae [Fig. 4]. The yeast was initially identified as Rhodotorula sp. Histopathological sections also showed the presence of yeast like cells [Fig. 5]. Antifungal sensitivity testing by disc diffusion method (CLSI document M44-A2) showed maximum sensitivity to Voriconazole; Amphotericin B and Natamycin showed intermediate activity while it was resistant to Itaconazole and Fluconazole. Recurrence of infection in the graft was noted as reappearance of hypopyon (3mm) along with a temporal full thickness stromal infiltration (3.2 mm × 2.8mm) involving the graft- host junction, after 18 days with isolation of same organism. Medical therapy with topical voriconazole (2%) was initiated along with voriconazole lavage of the anterior chamber, which led to resolution of the infection and subsequently to graft failure at the end of 3 months.


Rhodotorula mucilaginosa Keratitis: a rare fungus from Eastern India.

Saha S, Sengupta J, Chatterjee D, Banerjee D - Indian J Ophthalmol (2014)

Lactophenol cotton blue mount in 100X
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061676&req=5

Figure 4: Lactophenol cotton blue mount in 100X
Mentions: After 12 days of incubation orange pigmented glistening yeast like colonies were grown on SDA and PDA [Fig. 3]. Unicellular blastoconidia and elongated globose forms were seen in Grams staining from culture. Lactophenol cotton blue mount from the colonies showed capsulated yeast cells without hyphae [Fig. 4]. The yeast was initially identified as Rhodotorula sp. Histopathological sections also showed the presence of yeast like cells [Fig. 5]. Antifungal sensitivity testing by disc diffusion method (CLSI document M44-A2) showed maximum sensitivity to Voriconazole; Amphotericin B and Natamycin showed intermediate activity while it was resistant to Itaconazole and Fluconazole. Recurrence of infection in the graft was noted as reappearance of hypopyon (3mm) along with a temporal full thickness stromal infiltration (3.2 mm × 2.8mm) involving the graft- host junction, after 18 days with isolation of same organism. Medical therapy with topical voriconazole (2%) was initiated along with voriconazole lavage of the anterior chamber, which led to resolution of the infection and subsequently to graft failure at the end of 3 months.

Bottom Line: Molecular identification of the organism was performed which showed 100% homology with Rhodotorula mucilaginosa.Management of these cases is difficult often necessitating surgical procedures.However further reports are necessary to understand the disease and establish a treatment protocol.

View Article: PubMed Central - PubMed

Affiliation: Ocular Microbiology Laboratory Services, Priyamvada Birla Aravind Eye Hospital, 10 Loudon Street, Kolkata; Department of Microbiology, Vidyasagar University, Midnapore, India.

ABSTRACT
Rhodotorula mucilaginosa rarely cause keratitis in immunocompromised individuals. A 30 year old male with history of minor trauma presented with cotton wool like stromal infiltration and hypopyon in left eye. Microbiological examination of corneal scraping showed fungal hyphae and yeast cells in direct smear. Molecular identification of the organism was performed which showed 100% homology with Rhodotorula mucilaginosa. Management of these cases is difficult often necessitating surgical procedures. However further reports are necessary to understand the disease and establish a treatment protocol.

Show MeSH
Related in: MedlinePlus