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Intraocular soluble intracellular adhesion molecule-1 correlates with subretinal fluid height of diabetic macular edema.

Zhu D, Zhu H, Wang C, Yang D - Indian J Ophthalmol (2014)

Bottom Line: Maximum height of submacular fluid measured by Optical coherence tomography (OCT) was significantly associated with aqueous sICAM-1 (r = -0.45, P = 0.034).The maximum height of macular thickness measured by OCT was not significantly associated with either VEGF (P = 0.300), MCP-1 (P = 0.320) or sICAM-1 (P = 0.285).Our results suggest that sICAM-1 may majorly contribute to the formation of subretinal fluid in DME patients and imply that MCP-1 and sICAM-1 may be the potential therapy targets, besides VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, the Affiliated Hospital of Inner Mongolia Medical College, Hohhot, Inner Mongolia, China.

ABSTRACT

Objective: To investigate the correlations between aqueous concentrations of vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP-1), soluble intracellular adhesion molecule-1 (sICAM-1) and diabetic macular edema (DME).

Materials and methods: VEGF, MCP-1 and sICAM-1 concentrations in aqueous humor samples of 22 patients with DME and 23 patients with cataract of a control group were measured with solid-phase chemiluminescence immunoassay.

Results: Aqueous VEGF (89.2 ± 58.5 pg/ml versus 48.5 ± 27.8 pg/ml, P = 0.006), MCP-1 (684.2 ± 423.4 pg/ml versus 432.4 ± 230.4 pg/ml, P = 0.019) and sICAM-1 (3213.8 ± 2581.6 pg/ml versus 260.2 ± 212.2 pg/ml, P < 0.001) all vary significantly between DME group and control group. Maximum height of submacular fluid measured by Optical coherence tomography (OCT) was significantly associated with aqueous sICAM-1 (r = -0.45, P = 0.034). The maximum height of macular thickness measured by OCT was not significantly associated with either VEGF (P = 0.300), MCP-1 (P = 0.320) or sICAM-1 (P = 0.285).

Conclusions: Our results suggest that sICAM-1 may majorly contribute to the formation of subretinal fluid in DME patients and imply that MCP-1 and sICAM-1 may be the potential therapy targets, besides VEGF.

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Related in: MedlinePlus

Error bar showing the soluble intracellular adhesion molecule-1 in aqueous humor between diabetic macular edema patients without subretinal fluid (n = 16) and diabetic macular edema patients with subretinal fluid (n = 6) (independent t-test, 4032.9 ± 2570.8 pg/ml versus 1029.6 ± 542.1 pg/ml; P < 0.001)
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Figure 5: Error bar showing the soluble intracellular adhesion molecule-1 in aqueous humor between diabetic macular edema patients without subretinal fluid (n = 16) and diabetic macular edema patients with subretinal fluid (n = 6) (independent t-test, 4032.9 ± 2570.8 pg/ml versus 1029.6 ± 542.1 pg/ml; P < 0.001)

Mentions: Pearson correlation test showed that the maximum height of submacular fluid measured by OCT was significantly associated with aqueous sICAM-1 (r = -0.45, P = 0.034; Fig. 4), and was not significantly associated with aqueous VEGF (P = 0.395) and MCP-1 (P = 0.613). According to the presence of subretinal fluid in macula, the DME patients were further divided into two groups (6 patients with submacular fluid and 16 patients without submacular fluid), and independent t-test showed that only sICAM-1 varied significantly between the two groups (Fig. 5, 1029.6 ± 542.1 pg/ml (95%Confidence interval: 460.7 pg/ml–1598.5 pg/ml) versus 4032.9 ± 2570.8 pg/ml (95% Confidence interval: 2663.0 pg/ml–5402.8 pg/ml); P < 0.001). MCP-1 (700.2 ± 463.2 pg/ml (95%Confidence interval: 214.1 pg/ml–1186.4 pg/ml) versus 678.2 ± 423.5 pg/ml (95%Confidence interval: 452.6 pg/ml–903.9 pg/ml); P = 0.917) and VEGF (103.5 ± 73.4 pg/ml (95% Confidence interval: 26.4 pg/ml–180.5 pg/ml) versus 83.8 ± 53.8 pg/ml (95%Confidence interval: 55.2 pg/ml–112.5 pg/ml); P = 0.497) did not vary significantly between the two groups.


Intraocular soluble intracellular adhesion molecule-1 correlates with subretinal fluid height of diabetic macular edema.

Zhu D, Zhu H, Wang C, Yang D - Indian J Ophthalmol (2014)

Error bar showing the soluble intracellular adhesion molecule-1 in aqueous humor between diabetic macular edema patients without subretinal fluid (n = 16) and diabetic macular edema patients with subretinal fluid (n = 6) (independent t-test, 4032.9 ± 2570.8 pg/ml versus 1029.6 ± 542.1 pg/ml; P < 0.001)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061666&req=5

Figure 5: Error bar showing the soluble intracellular adhesion molecule-1 in aqueous humor between diabetic macular edema patients without subretinal fluid (n = 16) and diabetic macular edema patients with subretinal fluid (n = 6) (independent t-test, 4032.9 ± 2570.8 pg/ml versus 1029.6 ± 542.1 pg/ml; P < 0.001)
Mentions: Pearson correlation test showed that the maximum height of submacular fluid measured by OCT was significantly associated with aqueous sICAM-1 (r = -0.45, P = 0.034; Fig. 4), and was not significantly associated with aqueous VEGF (P = 0.395) and MCP-1 (P = 0.613). According to the presence of subretinal fluid in macula, the DME patients were further divided into two groups (6 patients with submacular fluid and 16 patients without submacular fluid), and independent t-test showed that only sICAM-1 varied significantly between the two groups (Fig. 5, 1029.6 ± 542.1 pg/ml (95%Confidence interval: 460.7 pg/ml–1598.5 pg/ml) versus 4032.9 ± 2570.8 pg/ml (95% Confidence interval: 2663.0 pg/ml–5402.8 pg/ml); P < 0.001). MCP-1 (700.2 ± 463.2 pg/ml (95%Confidence interval: 214.1 pg/ml–1186.4 pg/ml) versus 678.2 ± 423.5 pg/ml (95%Confidence interval: 452.6 pg/ml–903.9 pg/ml); P = 0.917) and VEGF (103.5 ± 73.4 pg/ml (95% Confidence interval: 26.4 pg/ml–180.5 pg/ml) versus 83.8 ± 53.8 pg/ml (95%Confidence interval: 55.2 pg/ml–112.5 pg/ml); P = 0.497) did not vary significantly between the two groups.

Bottom Line: Maximum height of submacular fluid measured by Optical coherence tomography (OCT) was significantly associated with aqueous sICAM-1 (r = -0.45, P = 0.034).The maximum height of macular thickness measured by OCT was not significantly associated with either VEGF (P = 0.300), MCP-1 (P = 0.320) or sICAM-1 (P = 0.285).Our results suggest that sICAM-1 may majorly contribute to the formation of subretinal fluid in DME patients and imply that MCP-1 and sICAM-1 may be the potential therapy targets, besides VEGF.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, the Affiliated Hospital of Inner Mongolia Medical College, Hohhot, Inner Mongolia, China.

ABSTRACT

Objective: To investigate the correlations between aqueous concentrations of vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP-1), soluble intracellular adhesion molecule-1 (sICAM-1) and diabetic macular edema (DME).

Materials and methods: VEGF, MCP-1 and sICAM-1 concentrations in aqueous humor samples of 22 patients with DME and 23 patients with cataract of a control group were measured with solid-phase chemiluminescence immunoassay.

Results: Aqueous VEGF (89.2 ± 58.5 pg/ml versus 48.5 ± 27.8 pg/ml, P = 0.006), MCP-1 (684.2 ± 423.4 pg/ml versus 432.4 ± 230.4 pg/ml, P = 0.019) and sICAM-1 (3213.8 ± 2581.6 pg/ml versus 260.2 ± 212.2 pg/ml, P < 0.001) all vary significantly between DME group and control group. Maximum height of submacular fluid measured by Optical coherence tomography (OCT) was significantly associated with aqueous sICAM-1 (r = -0.45, P = 0.034). The maximum height of macular thickness measured by OCT was not significantly associated with either VEGF (P = 0.300), MCP-1 (P = 0.320) or sICAM-1 (P = 0.285).

Conclusions: Our results suggest that sICAM-1 may majorly contribute to the formation of subretinal fluid in DME patients and imply that MCP-1 and sICAM-1 may be the potential therapy targets, besides VEGF.

Show MeSH
Related in: MedlinePlus