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Comparative pharmacognosy of Pashanbhed.

Verma P, Gauttam V, Kalia AN - J Ayurveda Integr Med (2014)

Bottom Line: The effect on urine output and electrolyte changes were measured for 24 h and compared.However, in intercomparison of the ME C. aromaticus (1,000 mg/kg p.o.) produced more significant diuresis (P < 0.05) and electrolyte excretion compared to other test groups, the effect was at par with furosemide.This suggests the use of C. aromaticus leaves as "Pashanbhed"; the most effective diuretic drug.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Indo Soviet Friendship College of Pharmacy, Moga, Punjab, India.

ABSTRACT

Background: Pashanbhed is a commercially available diuretic and lithotropic drug, used to treat renal problems. It is a controversial name as it is assigned to various plants such as Bergenia ligulata, Kalanchoe pinnata, Coleus aromaticus and Rotula aquatica.

Objective: To perform the comparative preliminary phytochemical screening, diuretic activity, and thin layer chromatography (TLC) finger printing profile of three plants (B. ligulata, C. aromaticus, and K. pinnata), most commonly used as Pashanbhed.

Materials and methods: Diuretic potential of methanolic extract (ME) of three plants were evaluated at two dose levels (500 and 1,000 mg/kg p.o.), using normal Wistar rats (Lipschitz method). Furosemide (20 mg/kg p.o.) was used as a standard drug. The effect on urine output and electrolyte changes were measured for 24 h and compared. All MEs were screened preliminarily for their constituents and their TLC finger printing profiles were prepared. One-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison test. P < 0.05 was considered statistically significant.

Results: The MEs of all three plants have shown diuresis in normal rats. However, in intercomparison of the ME C. aromaticus (1,000 mg/kg p.o.) produced more significant diuresis (P < 0.05) and electrolyte excretion compared to other test groups, the effect was at par with furosemide. The ME of these plants showed presence of alkaloids, glycosides, steroids, terpenoids, saponins, flavonoids, etc.

Conclusion: The ME of C. aromaticus (1,000 mg/kg p.o.) has showed highest diuretic action (4.2) among the tested extracts. This suggests the use of C. aromaticus leaves as "Pashanbhed"; the most effective diuretic drug.

No MeSH data available.


Related in: MedlinePlus

(a) TLC finger printing profiles of ME of Bergenia ligulata, Kalanchoe pinnata, and Coleus aromaticus, respectively, at short UV (254 nm). (b) TLC finger printing profiles of ME of B. ligulata, K. pinnata and C. aromaticus, respectively, at long UV (366 nm). (c) TLC finger printing profiles of ME of B. ligulata, K. pinnata, and C. aromaticus, respectively, at visible light
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Figure 1: (a) TLC finger printing profiles of ME of Bergenia ligulata, Kalanchoe pinnata, and Coleus aromaticus, respectively, at short UV (254 nm). (b) TLC finger printing profiles of ME of B. ligulata, K. pinnata and C. aromaticus, respectively, at long UV (366 nm). (c) TLC finger printing profiles of ME of B. ligulata, K. pinnata, and C. aromaticus, respectively, at visible light

Mentions: The TLC fingerprinting profile of B. ligulata, K. pinnata, and C. aromaticus had shown seven, five, and three spots at short UV [Figure 1a]; four, four, and three spots at long UV [Figure 1b]; and four, five, and three spots in visible light [Figure 1c]; respectively; with different Rf values [Table 1].


Comparative pharmacognosy of Pashanbhed.

Verma P, Gauttam V, Kalia AN - J Ayurveda Integr Med (2014)

(a) TLC finger printing profiles of ME of Bergenia ligulata, Kalanchoe pinnata, and Coleus aromaticus, respectively, at short UV (254 nm). (b) TLC finger printing profiles of ME of B. ligulata, K. pinnata and C. aromaticus, respectively, at long UV (366 nm). (c) TLC finger printing profiles of ME of B. ligulata, K. pinnata, and C. aromaticus, respectively, at visible light
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061584&req=5

Figure 1: (a) TLC finger printing profiles of ME of Bergenia ligulata, Kalanchoe pinnata, and Coleus aromaticus, respectively, at short UV (254 nm). (b) TLC finger printing profiles of ME of B. ligulata, K. pinnata and C. aromaticus, respectively, at long UV (366 nm). (c) TLC finger printing profiles of ME of B. ligulata, K. pinnata, and C. aromaticus, respectively, at visible light
Mentions: The TLC fingerprinting profile of B. ligulata, K. pinnata, and C. aromaticus had shown seven, five, and three spots at short UV [Figure 1a]; four, four, and three spots at long UV [Figure 1b]; and four, five, and three spots in visible light [Figure 1c]; respectively; with different Rf values [Table 1].

Bottom Line: The effect on urine output and electrolyte changes were measured for 24 h and compared.However, in intercomparison of the ME C. aromaticus (1,000 mg/kg p.o.) produced more significant diuresis (P < 0.05) and electrolyte excretion compared to other test groups, the effect was at par with furosemide.This suggests the use of C. aromaticus leaves as "Pashanbhed"; the most effective diuretic drug.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Indo Soviet Friendship College of Pharmacy, Moga, Punjab, India.

ABSTRACT

Background: Pashanbhed is a commercially available diuretic and lithotropic drug, used to treat renal problems. It is a controversial name as it is assigned to various plants such as Bergenia ligulata, Kalanchoe pinnata, Coleus aromaticus and Rotula aquatica.

Objective: To perform the comparative preliminary phytochemical screening, diuretic activity, and thin layer chromatography (TLC) finger printing profile of three plants (B. ligulata, C. aromaticus, and K. pinnata), most commonly used as Pashanbhed.

Materials and methods: Diuretic potential of methanolic extract (ME) of three plants were evaluated at two dose levels (500 and 1,000 mg/kg p.o.), using normal Wistar rats (Lipschitz method). Furosemide (20 mg/kg p.o.) was used as a standard drug. The effect on urine output and electrolyte changes were measured for 24 h and compared. All MEs were screened preliminarily for their constituents and their TLC finger printing profiles were prepared. One-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison test. P < 0.05 was considered statistically significant.

Results: The MEs of all three plants have shown diuresis in normal rats. However, in intercomparison of the ME C. aromaticus (1,000 mg/kg p.o.) produced more significant diuresis (P < 0.05) and electrolyte excretion compared to other test groups, the effect was at par with furosemide. The ME of these plants showed presence of alkaloids, glycosides, steroids, terpenoids, saponins, flavonoids, etc.

Conclusion: The ME of C. aromaticus (1,000 mg/kg p.o.) has showed highest diuretic action (4.2) among the tested extracts. This suggests the use of C. aromaticus leaves as "Pashanbhed"; the most effective diuretic drug.

No MeSH data available.


Related in: MedlinePlus