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Brazilian green propolis modulates inflammation, angiogenesis and fibrogenesis in intraperitoneal implant in mice.

Lima LD, Andrade SP, Campos PP, Barcelos LS, Soriani FM, Moura SA, Ferreira MA - BMC Complement Altern Med (2014)

Bottom Line: Propolis was able to decrease intraperitoneal permeability.Conversely, the treatment up-regulated inflammatory enzyme activities, TNF-α levels and gene expression of NOS2 and IFN-γ (23 and 7 fold, respectively), and of FIZZ1 and YM1 (8 and 2 fold) when compared with the untreated group.These observations show for the first time the effects of propolis modulating intraperitoneal inflammatory angiogenesis in mice and disclose important action mechanisms of the compound (downregulation of angiogenic components and activation of murine macrophage pathways).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Antônio Carlos 6627- Pampulha, Belo Horizonte, Minas Gerais CEP 31,270-901, Brazil. andrades@icb.ufmg.br.

ABSTRACT

Background: Chronic inflammatory processes in the peritoneal cavity develop as a result of ischemia, foreign body reaction, and trauma. Brazilian green propolis, a beeswax product, has been shown to exhibit multiple actions on inflammation and tissue repair. Our aim was to investigate the effects of this natural product on the inflammatory, angiogenic, and fibrogenic components of the peritoneal fibroproliferative tissue induced by a synthetic matrix.

Methods: Chronic inflammation was induced by placing polyether-polyurethane sponge discs in the abdominal cavity of anesthetized Swiss mice. Oral administration of propolis (500/mg/kg/day) by gavage started 24 hours after injury for four days. The effect of propolis on peritoneal permeability was evaluated through fluorescein diffusion rate 4 days post implantation. The effects of propolis on the inflammatory (myeloperoxidase and n-acetyl-β-D-glucosaminidase activities and TNF-α levels), angiogenic (hemoglobin content-Hb), and fibrogenic (TGF-β1 and collagen deposition) components of the fibrovascular tissue in the implants were determined 5 days after the injury.

Results: Propolis was able to decrease intraperitoneal permeability. The time taken for fluorescence to peak in the systemic circulation was 20±1 min in the treated group in contrast with 15±1 min in the control group. In addition, the treatment was shown to down-regulate angiogenesis (Hb content) and fibrosis by decreasing TGF-β1 levels and collagen deposition in fibroproliferative tissue induced by the synthetic implants. Conversely, the treatment up-regulated inflammatory enzyme activities, TNF-α levels and gene expression of NOS2 and IFN-γ (23 and 7 fold, respectively), and of FIZZ1 and YM1 (8 and 2 fold) when compared with the untreated group.

Conclusions: These observations show for the first time the effects of propolis modulating intraperitoneal inflammatory angiogenesis in mice and disclose important action mechanisms of the compound (downregulation of angiogenic components and activation of murine macrophage pathways).

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Effects of WEP on diffusion rate. WEP (500 mg/kg/day) on diffusion rate of sodium fluorescein applied intraperitoneally; (A), on angiogenesis (B and C) and on fibrogenesis components of intraperitoneal implants (D and E). Values shown are the means (±SEM) from f 10 animals in both groups. *p < 0.05 versus control group.
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Figure 2: Effects of WEP on diffusion rate. WEP (500 mg/kg/day) on diffusion rate of sodium fluorescein applied intraperitoneally; (A), on angiogenesis (B and C) and on fibrogenesis components of intraperitoneal implants (D and E). Values shown are the means (±SEM) from f 10 animals in both groups. *p < 0.05 versus control group.

Mentions: Systemic treatment with propolis for 4 days was able to lower the rate of fluorescein diffusion from the peritoneal cavity to the systemic circulation when compared with the untreated animals. It took 20 ± 1 min for the fluorescence to peak in the systemic circulation in the treated group and 15 ± 1 min in the control group (Figure 2A).


Brazilian green propolis modulates inflammation, angiogenesis and fibrogenesis in intraperitoneal implant in mice.

Lima LD, Andrade SP, Campos PP, Barcelos LS, Soriani FM, Moura SA, Ferreira MA - BMC Complement Altern Med (2014)

Effects of WEP on diffusion rate. WEP (500 mg/kg/day) on diffusion rate of sodium fluorescein applied intraperitoneally; (A), on angiogenesis (B and C) and on fibrogenesis components of intraperitoneal implants (D and E). Values shown are the means (±SEM) from f 10 animals in both groups. *p < 0.05 versus control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4061536&req=5

Figure 2: Effects of WEP on diffusion rate. WEP (500 mg/kg/day) on diffusion rate of sodium fluorescein applied intraperitoneally; (A), on angiogenesis (B and C) and on fibrogenesis components of intraperitoneal implants (D and E). Values shown are the means (±SEM) from f 10 animals in both groups. *p < 0.05 versus control group.
Mentions: Systemic treatment with propolis for 4 days was able to lower the rate of fluorescein diffusion from the peritoneal cavity to the systemic circulation when compared with the untreated animals. It took 20 ± 1 min for the fluorescence to peak in the systemic circulation in the treated group and 15 ± 1 min in the control group (Figure 2A).

Bottom Line: Propolis was able to decrease intraperitoneal permeability.Conversely, the treatment up-regulated inflammatory enzyme activities, TNF-α levels and gene expression of NOS2 and IFN-γ (23 and 7 fold, respectively), and of FIZZ1 and YM1 (8 and 2 fold) when compared with the untreated group.These observations show for the first time the effects of propolis modulating intraperitoneal inflammatory angiogenesis in mice and disclose important action mechanisms of the compound (downregulation of angiogenic components and activation of murine macrophage pathways).

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Antônio Carlos 6627- Pampulha, Belo Horizonte, Minas Gerais CEP 31,270-901, Brazil. andrades@icb.ufmg.br.

ABSTRACT

Background: Chronic inflammatory processes in the peritoneal cavity develop as a result of ischemia, foreign body reaction, and trauma. Brazilian green propolis, a beeswax product, has been shown to exhibit multiple actions on inflammation and tissue repair. Our aim was to investigate the effects of this natural product on the inflammatory, angiogenic, and fibrogenic components of the peritoneal fibroproliferative tissue induced by a synthetic matrix.

Methods: Chronic inflammation was induced by placing polyether-polyurethane sponge discs in the abdominal cavity of anesthetized Swiss mice. Oral administration of propolis (500/mg/kg/day) by gavage started 24 hours after injury for four days. The effect of propolis on peritoneal permeability was evaluated through fluorescein diffusion rate 4 days post implantation. The effects of propolis on the inflammatory (myeloperoxidase and n-acetyl-β-D-glucosaminidase activities and TNF-α levels), angiogenic (hemoglobin content-Hb), and fibrogenic (TGF-β1 and collagen deposition) components of the fibrovascular tissue in the implants were determined 5 days after the injury.

Results: Propolis was able to decrease intraperitoneal permeability. The time taken for fluorescence to peak in the systemic circulation was 20±1 min in the treated group in contrast with 15±1 min in the control group. In addition, the treatment was shown to down-regulate angiogenesis (Hb content) and fibrosis by decreasing TGF-β1 levels and collagen deposition in fibroproliferative tissue induced by the synthetic implants. Conversely, the treatment up-regulated inflammatory enzyme activities, TNF-α levels and gene expression of NOS2 and IFN-γ (23 and 7 fold, respectively), and of FIZZ1 and YM1 (8 and 2 fold) when compared with the untreated group.

Conclusions: These observations show for the first time the effects of propolis modulating intraperitoneal inflammatory angiogenesis in mice and disclose important action mechanisms of the compound (downregulation of angiogenic components and activation of murine macrophage pathways).

Show MeSH
Related in: MedlinePlus