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Reprogramming of cassava (Manihot esculenta) microspores towards sporophytic development.

Perera PI, Ordoñez CA, Dedicova B, Ortega PE - AoB Plants (2014)

Bottom Line: In two cassava genotypes, different developmental stages of microspores were found to initiate sporophytic cell divisions, that is, with tetrads of TMS 60444 and with mid or late uni-nucleate microspores of SM 1219-9.A low frequency of the reprogramming and the presence of non-responsive microspores among the responsive ones in tetrads were found to be related to the viability and exine formation of the microspores.This is the first ever detailed report of microspore reprogramming at the tetrad stage and the first report of microspore embryogenesis induction in cassava with detailed evidence.

View Article: PubMed Central - PubMed

Affiliation: Agrobiodiversity Research Area, International Center for Tropical Agriculture, A.A. 6713, Cali, Colombia Present address: Department of Horticulture and Landscape Gardening, Faculty of Agriculture and Plantation Management, Wayamba University, Gonawila, Makandura, Sri Lanka prasanthi1970@yahoo.com.

No MeSH data available.


Related in: MedlinePlus

Morphological aspects of isolated microspores from genotype SM1219-9 of M. esculenta. (A) The suspensions consisted of microspores of black (b) colour having a well-developed exine captured in the 70– to 104-µm extract. (B) Microspores with partially synthesized exine (pse) of less intense black and slightly synthesized exine (sse) in yellowish brown in the 41– to 70-µm extract.
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PLU022F1: Morphological aspects of isolated microspores from genotype SM1219-9 of M. esculenta. (A) The suspensions consisted of microspores of black (b) colour having a well-developed exine captured in the 70– to 104-µm extract. (B) Microspores with partially synthesized exine (pse) of less intense black and slightly synthesized exine (sse) in yellowish brown in the 41– to 70-µm extract.

Mentions: Freshly isolated microspores were separated into three size groups by filtering. In both genotypes, the tested groups of microspores were in the size ranges of 149–104, 104–70 and 70–41 µm, designated hereafter as 104, 70 and 41 extracts. An almost-clear debris-free extract of each size category was obtained by centrifuging the suspensions with 10 % followed by 30 % sucrose solution independently at different speeds: 104 and 70 extracts at 500 rpm and the 41 extract at 1000 rpm, each for 1 min. In the 104 and 70 extracts, the suspensions consisted of microspores of different sizes of black colour when viewed under an inverted microscope (Fig. 1A) with a well-developed exine, whereas in the 41–70 µm extract the microspores contained a partially synthesized exine of less intense black colour and a slightly synthesized exine of yellowish brown colour (Fig. 1B). Tetrads, mixed with free microspores in the mid or late uni-nucleate stage, were captured by the 70 extract.Figure 1.


Reprogramming of cassava (Manihot esculenta) microspores towards sporophytic development.

Perera PI, Ordoñez CA, Dedicova B, Ortega PE - AoB Plants (2014)

Morphological aspects of isolated microspores from genotype SM1219-9 of M. esculenta. (A) The suspensions consisted of microspores of black (b) colour having a well-developed exine captured in the 70– to 104-µm extract. (B) Microspores with partially synthesized exine (pse) of less intense black and slightly synthesized exine (sse) in yellowish brown in the 41– to 70-µm extract.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061485&req=5

PLU022F1: Morphological aspects of isolated microspores from genotype SM1219-9 of M. esculenta. (A) The suspensions consisted of microspores of black (b) colour having a well-developed exine captured in the 70– to 104-µm extract. (B) Microspores with partially synthesized exine (pse) of less intense black and slightly synthesized exine (sse) in yellowish brown in the 41– to 70-µm extract.
Mentions: Freshly isolated microspores were separated into three size groups by filtering. In both genotypes, the tested groups of microspores were in the size ranges of 149–104, 104–70 and 70–41 µm, designated hereafter as 104, 70 and 41 extracts. An almost-clear debris-free extract of each size category was obtained by centrifuging the suspensions with 10 % followed by 30 % sucrose solution independently at different speeds: 104 and 70 extracts at 500 rpm and the 41 extract at 1000 rpm, each for 1 min. In the 104 and 70 extracts, the suspensions consisted of microspores of different sizes of black colour when viewed under an inverted microscope (Fig. 1A) with a well-developed exine, whereas in the 41–70 µm extract the microspores contained a partially synthesized exine of less intense black colour and a slightly synthesized exine of yellowish brown colour (Fig. 1B). Tetrads, mixed with free microspores in the mid or late uni-nucleate stage, were captured by the 70 extract.Figure 1.

Bottom Line: In two cassava genotypes, different developmental stages of microspores were found to initiate sporophytic cell divisions, that is, with tetrads of TMS 60444 and with mid or late uni-nucleate microspores of SM 1219-9.A low frequency of the reprogramming and the presence of non-responsive microspores among the responsive ones in tetrads were found to be related to the viability and exine formation of the microspores.This is the first ever detailed report of microspore reprogramming at the tetrad stage and the first report of microspore embryogenesis induction in cassava with detailed evidence.

View Article: PubMed Central - PubMed

Affiliation: Agrobiodiversity Research Area, International Center for Tropical Agriculture, A.A. 6713, Cali, Colombia Present address: Department of Horticulture and Landscape Gardening, Faculty of Agriculture and Plantation Management, Wayamba University, Gonawila, Makandura, Sri Lanka prasanthi1970@yahoo.com.

No MeSH data available.


Related in: MedlinePlus