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Inhibitory effect of rape pollen supercritical CO2 fluid extract against testosterone-induced benign prostatic hyperplasia in rats.

Yang BC, Jin LL, Yang YF, Li K, Peng DM - Exp Ther Med (2014)

Bottom Line: However, rape pollen SFE-CO2 treatment resulted in significant reductions in the prostate index and testosterone, DHT, 5α-reductase and COX-2 levels compared with those in BPH-induced animals.These observations indicate that rape pollen SFE-CO2 inhibits the development of BPH in rats and these effects are closely associated with reductions in DHT, 5α-reductase and COX-2 levels.Therefore, the results of the present study clearly indicate that rape pollen SFE-CO2 extract may be a useful agent in BPH treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, P.R. China ; Key Laboratory of Women's Reproductive Health of Jiangxi Province, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi 330006, P.R. China.

ABSTRACT
Benign prostatic hyperplasia (BPH) can lead to lower urinary tract symptoms. Rape pollen is an apicultural product that is composed of nutritionally valuable and biologically active substances. The aim of the present study was to investigate the protective effect of rape pollen supercritical CO2 fluid extract (SFE-CO2) in BPH development using a testosterone-induced BPH rat model. BPH was induced in the experimental groups by daily subcutaneous injections of testosterone for a period of 30 days. Rape pollen SFE-CO2 was administered daily by oral gavage concurrently with the testosterone injections. Animals were sacrificed at the scheduled termination and the prostates were weighed and subjected to histopathological examination. Testosterone, dihydrotestosterone (DHT), 5α-reductase and cyclooxygenase-2 (COX-2) levels were also measured. BPH-induced animals exhibited an increase in prostate weight with increased testosterone, DHT, 5α-reductase and COX-2 expression levels. However, rape pollen SFE-CO2 treatment resulted in significant reductions in the prostate index and testosterone, DHT, 5α-reductase and COX-2 levels compared with those in BPH-induced animals. Histopathological examination also demonstrated that rape pollen SFE-CO2 treatment suppressed testosterone-induced BPH. These observations indicate that rape pollen SFE-CO2 inhibits the development of BPH in rats and these effects are closely associated with reductions in DHT, 5α-reductase and COX-2 levels. Therefore, the results of the present study clearly indicate that rape pollen SFE-CO2 extract may be a useful agent in BPH treatment.

No MeSH data available.


Related in: MedlinePlus

Effects of rape pollen SFE-CO2 on (A and C) testosterone and (B and D) DHT levels in (A and B) serum and (C and D) prostate. The rape pollen SFE-CO2 treatment groups exhibited significantly decreased testosterone and DHT levels in the serum and prostate compared with the BPH group. Rape pollen SFE-CO2 or finasteride treatment was administered 1 h prior to testosterone injection. #P<0.01, vs. castration; *P<0.05, vs. BPH. Castration, corn oil injection (sc) + PBS (p.o.); BPH, testosterone (sc) + PBS (p.o.); finasteride, 5 mg/kg finasteride (p.o.) + testosterone (sc); pollen (low), 21.3 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); pollen (high), 88.7 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); SFE, supercritical fluid extract; DHT, dihydrotestosterone; BPH, benign prostatic hyperplasia; PBS, phosphate-buffered saline.
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f1-etm-08-01-0031: Effects of rape pollen SFE-CO2 on (A and C) testosterone and (B and D) DHT levels in (A and B) serum and (C and D) prostate. The rape pollen SFE-CO2 treatment groups exhibited significantly decreased testosterone and DHT levels in the serum and prostate compared with the BPH group. Rape pollen SFE-CO2 or finasteride treatment was administered 1 h prior to testosterone injection. #P<0.01, vs. castration; *P<0.05, vs. BPH. Castration, corn oil injection (sc) + PBS (p.o.); BPH, testosterone (sc) + PBS (p.o.); finasteride, 5 mg/kg finasteride (p.o.) + testosterone (sc); pollen (low), 21.3 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); pollen (high), 88.7 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); SFE, supercritical fluid extract; DHT, dihydrotestosterone; BPH, benign prostatic hyperplasia; PBS, phosphate-buffered saline.

Mentions: As shown in Fig. 1A and B, the testosterone-induced BPH group had significantly increased serum testosterone levels compared with those in the castration group. However, the finasteride- and pollen-treated groups had significantly decreased serum testosterone levels compared with those in the testosterone-induced BPH group. Serum DHT levels in the testosterone-induced BPH group were significantly increased compared with those in the castration group. However, the serum DHT levels in the finasteride- and pollen-treated groups were significantly decreased compared with those in the testosterone-induced BPH group.


Inhibitory effect of rape pollen supercritical CO2 fluid extract against testosterone-induced benign prostatic hyperplasia in rats.

Yang BC, Jin LL, Yang YF, Li K, Peng DM - Exp Ther Med (2014)

Effects of rape pollen SFE-CO2 on (A and C) testosterone and (B and D) DHT levels in (A and B) serum and (C and D) prostate. The rape pollen SFE-CO2 treatment groups exhibited significantly decreased testosterone and DHT levels in the serum and prostate compared with the BPH group. Rape pollen SFE-CO2 or finasteride treatment was administered 1 h prior to testosterone injection. #P<0.01, vs. castration; *P<0.05, vs. BPH. Castration, corn oil injection (sc) + PBS (p.o.); BPH, testosterone (sc) + PBS (p.o.); finasteride, 5 mg/kg finasteride (p.o.) + testosterone (sc); pollen (low), 21.3 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); pollen (high), 88.7 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); SFE, supercritical fluid extract; DHT, dihydrotestosterone; BPH, benign prostatic hyperplasia; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061240&req=5

f1-etm-08-01-0031: Effects of rape pollen SFE-CO2 on (A and C) testosterone and (B and D) DHT levels in (A and B) serum and (C and D) prostate. The rape pollen SFE-CO2 treatment groups exhibited significantly decreased testosterone and DHT levels in the serum and prostate compared with the BPH group. Rape pollen SFE-CO2 or finasteride treatment was administered 1 h prior to testosterone injection. #P<0.01, vs. castration; *P<0.05, vs. BPH. Castration, corn oil injection (sc) + PBS (p.o.); BPH, testosterone (sc) + PBS (p.o.); finasteride, 5 mg/kg finasteride (p.o.) + testosterone (sc); pollen (low), 21.3 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); pollen (high), 88.7 mg/kg rape pollen SFE-CO2 (p.o.) + testosterone (sc); SFE, supercritical fluid extract; DHT, dihydrotestosterone; BPH, benign prostatic hyperplasia; PBS, phosphate-buffered saline.
Mentions: As shown in Fig. 1A and B, the testosterone-induced BPH group had significantly increased serum testosterone levels compared with those in the castration group. However, the finasteride- and pollen-treated groups had significantly decreased serum testosterone levels compared with those in the testosterone-induced BPH group. Serum DHT levels in the testosterone-induced BPH group were significantly increased compared with those in the castration group. However, the serum DHT levels in the finasteride- and pollen-treated groups were significantly decreased compared with those in the testosterone-induced BPH group.

Bottom Line: However, rape pollen SFE-CO2 treatment resulted in significant reductions in the prostate index and testosterone, DHT, 5α-reductase and COX-2 levels compared with those in BPH-induced animals.These observations indicate that rape pollen SFE-CO2 inhibits the development of BPH in rats and these effects are closely associated with reductions in DHT, 5α-reductase and COX-2 levels.Therefore, the results of the present study clearly indicate that rape pollen SFE-CO2 extract may be a useful agent in BPH treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, P.R. China ; Key Laboratory of Women's Reproductive Health of Jiangxi Province, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi 330006, P.R. China.

ABSTRACT
Benign prostatic hyperplasia (BPH) can lead to lower urinary tract symptoms. Rape pollen is an apicultural product that is composed of nutritionally valuable and biologically active substances. The aim of the present study was to investigate the protective effect of rape pollen supercritical CO2 fluid extract (SFE-CO2) in BPH development using a testosterone-induced BPH rat model. BPH was induced in the experimental groups by daily subcutaneous injections of testosterone for a period of 30 days. Rape pollen SFE-CO2 was administered daily by oral gavage concurrently with the testosterone injections. Animals were sacrificed at the scheduled termination and the prostates were weighed and subjected to histopathological examination. Testosterone, dihydrotestosterone (DHT), 5α-reductase and cyclooxygenase-2 (COX-2) levels were also measured. BPH-induced animals exhibited an increase in prostate weight with increased testosterone, DHT, 5α-reductase and COX-2 expression levels. However, rape pollen SFE-CO2 treatment resulted in significant reductions in the prostate index and testosterone, DHT, 5α-reductase and COX-2 levels compared with those in BPH-induced animals. Histopathological examination also demonstrated that rape pollen SFE-CO2 treatment suppressed testosterone-induced BPH. These observations indicate that rape pollen SFE-CO2 inhibits the development of BPH in rats and these effects are closely associated with reductions in DHT, 5α-reductase and COX-2 levels. Therefore, the results of the present study clearly indicate that rape pollen SFE-CO2 extract may be a useful agent in BPH treatment.

No MeSH data available.


Related in: MedlinePlus