Limits...
Direct identification of Mycobacterium abscessus through 16S rDNA sequence analysis and a citrate utilization test: A case report.

Zou Z, Liu Y, Zhu B, Zeng P - Exp Ther Med (2014)

Bottom Line: The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg.Routine cultures for fungus were repeatedly negative.The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.

ABSTRACT
A growing number of nontuberculous mycobacteria infection cases, especially those caused by rapidly growing mycobacteria (RGM), have been reported in the past decade. Conventional methods for mycobacteria diagnosis are inefficient and easily lead to misdiagnosis. New detection methods, such as gene sequencing, have been reported but are not widely used. The aim of the present case report was to provide a quick and exact method of identifying Myobacterium abscessus (M. abscessus) infections. The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg. Routine cultures for fungus were repeatedly negative. However, cultures of the purulent material under aerobic cultivation for five days yielded a rapidly growing, nontuberculous mycobacterium. A Ziehl-Neelsen staining of this mycobacterium revealed the presence of acid-fast bacilli that were finally identified as M. abscessus through 16S rDNA sequence analysis and a citrate utilization test. The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.

No MeSH data available.


Related in: MedlinePlus

Multiple skin lesions on the right leg of the patient at admission. There was seropurulent discharge from certain lesions, and some crusts were formed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4061238&req=5

f1-etm-08-01-0115: Multiple skin lesions on the right leg of the patient at admission. There was seropurulent discharge from certain lesions, and some crusts were formed.

Mentions: Inspection of the lower extremities revealed multiple, painless, purple-brown colored, circular and clearly delineated nodular lesions, 2×2 cm in size, which were localized to the lower right leg and foot (Fig. 1). Crimson liquid was exuding from certain lesions and some crusts had formed. Laboratory investigations revealed that the blood glucose level was normal. No abnormalities in the biochemical and urine tests were identified. Examination of autoantibodies also revealed no abnormalities and the X-rays of the chest were unremarkable. A plain film of the right leg revealed a small area of shadow in the soft tissue area, which was considered as a foreign material. Gross pathological changes in the bones and joints were not identified. Magnetic resonance angiography (MRA) of the lower extremity vasculature revealed that stenosis was present in the peroneal artery of the lower right leg. An ultrasound scan of the lower extremity vasculature demonstrated extensive thrombosis involving the right calf muscle veins. A skin biopsy revealed signs consistent with a suppurative inflammation process in the skin, with a large number of inflammatory cells (mainly small lymphocytes) present.


Direct identification of Mycobacterium abscessus through 16S rDNA sequence analysis and a citrate utilization test: A case report.

Zou Z, Liu Y, Zhu B, Zeng P - Exp Ther Med (2014)

Multiple skin lesions on the right leg of the patient at admission. There was seropurulent discharge from certain lesions, and some crusts were formed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061238&req=5

f1-etm-08-01-0115: Multiple skin lesions on the right leg of the patient at admission. There was seropurulent discharge from certain lesions, and some crusts were formed.
Mentions: Inspection of the lower extremities revealed multiple, painless, purple-brown colored, circular and clearly delineated nodular lesions, 2×2 cm in size, which were localized to the lower right leg and foot (Fig. 1). Crimson liquid was exuding from certain lesions and some crusts had formed. Laboratory investigations revealed that the blood glucose level was normal. No abnormalities in the biochemical and urine tests were identified. Examination of autoantibodies also revealed no abnormalities and the X-rays of the chest were unremarkable. A plain film of the right leg revealed a small area of shadow in the soft tissue area, which was considered as a foreign material. Gross pathological changes in the bones and joints were not identified. Magnetic resonance angiography (MRA) of the lower extremity vasculature revealed that stenosis was present in the peroneal artery of the lower right leg. An ultrasound scan of the lower extremity vasculature demonstrated extensive thrombosis involving the right calf muscle veins. A skin biopsy revealed signs consistent with a suppurative inflammation process in the skin, with a large number of inflammatory cells (mainly small lymphocytes) present.

Bottom Line: The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg.Routine cultures for fungus were repeatedly negative.The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China.

ABSTRACT
A growing number of nontuberculous mycobacteria infection cases, especially those caused by rapidly growing mycobacteria (RGM), have been reported in the past decade. Conventional methods for mycobacteria diagnosis are inefficient and easily lead to misdiagnosis. New detection methods, such as gene sequencing, have been reported but are not widely used. The aim of the present case report was to provide a quick and exact method of identifying Myobacterium abscessus (M. abscessus) infections. The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg. Routine cultures for fungus were repeatedly negative. However, cultures of the purulent material under aerobic cultivation for five days yielded a rapidly growing, nontuberculous mycobacterium. A Ziehl-Neelsen staining of this mycobacterium revealed the presence of acid-fast bacilli that were finally identified as M. abscessus through 16S rDNA sequence analysis and a citrate utilization test. The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.

No MeSH data available.


Related in: MedlinePlus