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Ethanol extract of Cirsium japonicum attenuates hepatic lipid accumulation via AMPK activation in human HepG2 cells.

Wan Y, Liu LY, Hong ZF, Peng J - Exp Ther Med (2014)

Bottom Line: One of the most common causes of chronic liver disease, nonalcoholic fatty liver disease (NAFLD), is strongly associated with obesity and dysregulated insulin action in the liver.A flowering plant in the Asteraceae family, Cirsium japonicum (CJ), exhibits a variety of pharmacological and antioxidative properties that promote hepatoprotection.In the present study, CJ ethanol extract was shown to reduce hepatic triglyceride (TG) and cholesterol accumulation.

View Article: PubMed Central - PubMed

Affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT
One of the most common causes of chronic liver disease, nonalcoholic fatty liver disease (NAFLD), is strongly associated with obesity and dysregulated insulin action in the liver. However, there are no pharmacological agents currently established for the treatment of NAFLD. A flowering plant in the Asteraceae family, Cirsium japonicum (CJ), exhibits a variety of pharmacological and antioxidative properties that promote hepatoprotection. In the present study, CJ ethanol extract was shown to reduce hepatic triglyceride (TG) and cholesterol accumulation. CJ significantly increased AMP-activated protein kinase (AMPK) phosphorylation in HepG2 hepatocytes and downregulated the level of the target genes, acetyl-CoA carboxylase and fatty acid synthase. In addition, CJ upregulated the expression of carnitine palmitoyltransferase-1, which is involved in fatty acid oxidation. The results of the present study indicated that the positive effects of CJ extract on high-fat diet-induced hepatic TG accumulation were mediated via the AMPK signaling pathway, indicating a potential target for the preventative treatment of NAFLD.

No MeSH data available.


Related in: MedlinePlus

Effects of CJ and HFFA on HepG2 cells. (A) HepG2 cells were incubated with or without CJ at various concentrations and the cell viability was measured using the MTT assay. (B) Effect of HFFA on cytotoxicity in HepG2 cells. Data are expressed as the mean ± SE (bars) of three independent experiments. *P<0.05, vs. control. CJ, Cirsium japonicum; HFFA, free fatty acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
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f1-etm-08-01-0079: Effects of CJ and HFFA on HepG2 cells. (A) HepG2 cells were incubated with or without CJ at various concentrations and the cell viability was measured using the MTT assay. (B) Effect of HFFA on cytotoxicity in HepG2 cells. Data are expressed as the mean ± SE (bars) of three independent experiments. *P<0.05, vs. control. CJ, Cirsium japonicum; HFFA, free fatty acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Mentions: The concentration dependence of the potential cytotoxicity of CJ in HepG2 cells in the absence or presence of 1 mmol/l FFA for 24 h was firstly determined using an MTT assay. CJ exhibited between 1.7 and 12.4% cytotoxicity in HepG2 cells at the 0–2 mg/ml concentration range when incubated for 24 and 48 h, as shown in Fig. 1A. When treated with HFFA for 24 h to induce conditions of hepatic steatosis, 14.3% cytotoxicity was observed in the cells. Nevertheless, no significant cytotoxicity was imparted by CJ, as demonstrated in Fig. 1B.


Ethanol extract of Cirsium japonicum attenuates hepatic lipid accumulation via AMPK activation in human HepG2 cells.

Wan Y, Liu LY, Hong ZF, Peng J - Exp Ther Med (2014)

Effects of CJ and HFFA on HepG2 cells. (A) HepG2 cells were incubated with or without CJ at various concentrations and the cell viability was measured using the MTT assay. (B) Effect of HFFA on cytotoxicity in HepG2 cells. Data are expressed as the mean ± SE (bars) of three independent experiments. *P<0.05, vs. control. CJ, Cirsium japonicum; HFFA, free fatty acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061235&req=5

f1-etm-08-01-0079: Effects of CJ and HFFA on HepG2 cells. (A) HepG2 cells were incubated with or without CJ at various concentrations and the cell viability was measured using the MTT assay. (B) Effect of HFFA on cytotoxicity in HepG2 cells. Data are expressed as the mean ± SE (bars) of three independent experiments. *P<0.05, vs. control. CJ, Cirsium japonicum; HFFA, free fatty acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
Mentions: The concentration dependence of the potential cytotoxicity of CJ in HepG2 cells in the absence or presence of 1 mmol/l FFA for 24 h was firstly determined using an MTT assay. CJ exhibited between 1.7 and 12.4% cytotoxicity in HepG2 cells at the 0–2 mg/ml concentration range when incubated for 24 and 48 h, as shown in Fig. 1A. When treated with HFFA for 24 h to induce conditions of hepatic steatosis, 14.3% cytotoxicity was observed in the cells. Nevertheless, no significant cytotoxicity was imparted by CJ, as demonstrated in Fig. 1B.

Bottom Line: One of the most common causes of chronic liver disease, nonalcoholic fatty liver disease (NAFLD), is strongly associated with obesity and dysregulated insulin action in the liver.A flowering plant in the Asteraceae family, Cirsium japonicum (CJ), exhibits a variety of pharmacological and antioxidative properties that promote hepatoprotection.In the present study, CJ ethanol extract was shown to reduce hepatic triglyceride (TG) and cholesterol accumulation.

View Article: PubMed Central - PubMed

Affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT
One of the most common causes of chronic liver disease, nonalcoholic fatty liver disease (NAFLD), is strongly associated with obesity and dysregulated insulin action in the liver. However, there are no pharmacological agents currently established for the treatment of NAFLD. A flowering plant in the Asteraceae family, Cirsium japonicum (CJ), exhibits a variety of pharmacological and antioxidative properties that promote hepatoprotection. In the present study, CJ ethanol extract was shown to reduce hepatic triglyceride (TG) and cholesterol accumulation. CJ significantly increased AMP-activated protein kinase (AMPK) phosphorylation in HepG2 hepatocytes and downregulated the level of the target genes, acetyl-CoA carboxylase and fatty acid synthase. In addition, CJ upregulated the expression of carnitine palmitoyltransferase-1, which is involved in fatty acid oxidation. The results of the present study indicated that the positive effects of CJ extract on high-fat diet-induced hepatic TG accumulation were mediated via the AMPK signaling pathway, indicating a potential target for the preventative treatment of NAFLD.

No MeSH data available.


Related in: MedlinePlus