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In vivo determination of muscle-derived stem cells in the rat corpus cavernosum.

Xu L, Xue B, Shan Y, Chen D, Gao J, Yang D, Sun C, Cui Y - Exp Ther Med (2014)

Bottom Line: A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus.The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05).In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum.

View Article: PubMed Central - PubMed

Affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.

ABSTRACT
The aim of the present in vivo study was to determine the presence of muscle-derived stem cells (MDSCs) in the corpus cavernosum of rats. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were performed to detect the expression of the stem cell markers stem cell antigen-1 (Sca-1), Oct4 and Desmin in Sprague-Dawley rats aged 2, 5 and 20 months. Sca-1 was mainly expressed in the blood vessels and cavernous sinus and staining revealed that Sca-1 was predominantly expressed in the cytoplasm. Desmin was primarily expressed in muscular tissues and staining demonstrated that it was mainly expressed in the cytoplasm, however, Desmin was also partially expressed in the nuclei. A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus. It was found that the expression of the markers was negatively correlated with the age of the rats (P<0.05). The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05). In addition, the correlation analysis indicated that the expression of Sca-1 and Desmin were negatively correlated with the age of the rats (r=--0.929; P<0.05). In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum. MDSCs may be a potential therapeutic treatment for organic erectile dysfunction.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of Sca-1, Oct4 and Desmin expression in PP1–PP6 cells. PP, pre-plate; Sca-1, stem cell antigen-1.
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f6-etm-08-01-0274: Western blot analysis of Sca-1, Oct4 and Desmin expression in PP1–PP6 cells. PP, pre-plate; Sca-1, stem cell antigen-1.

Mentions: The expression of Sca-1, Oct4 and Desmin was detected in PP6 cells, however, the expression of Sca-1 and Oct4 was only detected in a few cells. The expression of Sca-1, Oct4 and Desmin in PP6 cells was 5.7, 2.6 and 41.2% respectively (Fig. 5). Sca-1 and Desmin were primarily expressed in the cytoplasm, whilst Oct4 was primarily expressed in the nuclei (Fig. 6). A small number of cells expressed Sca-1/Oct4, Sca-1/Desmin and Oct4/Desmin. Sca-1/Oct4 were expressed in the cytoplasm and the nuclei, whilst Sca-1/Desmin were primarily expressed in the cytoplasm. Oct4/Desmin were mainly expressed in the nuclei and the cytoplasm.


In vivo determination of muscle-derived stem cells in the rat corpus cavernosum.

Xu L, Xue B, Shan Y, Chen D, Gao J, Yang D, Sun C, Cui Y - Exp Ther Med (2014)

Western blot analysis of Sca-1, Oct4 and Desmin expression in PP1–PP6 cells. PP, pre-plate; Sca-1, stem cell antigen-1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061231&req=5

f6-etm-08-01-0274: Western blot analysis of Sca-1, Oct4 and Desmin expression in PP1–PP6 cells. PP, pre-plate; Sca-1, stem cell antigen-1.
Mentions: The expression of Sca-1, Oct4 and Desmin was detected in PP6 cells, however, the expression of Sca-1 and Oct4 was only detected in a few cells. The expression of Sca-1, Oct4 and Desmin in PP6 cells was 5.7, 2.6 and 41.2% respectively (Fig. 5). Sca-1 and Desmin were primarily expressed in the cytoplasm, whilst Oct4 was primarily expressed in the nuclei (Fig. 6). A small number of cells expressed Sca-1/Oct4, Sca-1/Desmin and Oct4/Desmin. Sca-1/Oct4 were expressed in the cytoplasm and the nuclei, whilst Sca-1/Desmin were primarily expressed in the cytoplasm. Oct4/Desmin were mainly expressed in the nuclei and the cytoplasm.

Bottom Line: A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus.The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05).In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum.

View Article: PubMed Central - PubMed

Affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.

ABSTRACT
The aim of the present in vivo study was to determine the presence of muscle-derived stem cells (MDSCs) in the corpus cavernosum of rats. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were performed to detect the expression of the stem cell markers stem cell antigen-1 (Sca-1), Oct4 and Desmin in Sprague-Dawley rats aged 2, 5 and 20 months. Sca-1 was mainly expressed in the blood vessels and cavernous sinus and staining revealed that Sca-1 was predominantly expressed in the cytoplasm. Desmin was primarily expressed in muscular tissues and staining demonstrated that it was mainly expressed in the cytoplasm, however, Desmin was also partially expressed in the nuclei. A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus. It was found that the expression of the markers was negatively correlated with the age of the rats (P<0.05). The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05). In addition, the correlation analysis indicated that the expression of Sca-1 and Desmin were negatively correlated with the age of the rats (r=--0.929; P<0.05). In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum. MDSCs may be a potential therapeutic treatment for organic erectile dysfunction.

No MeSH data available.


Related in: MedlinePlus