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In vivo determination of muscle-derived stem cells in the rat corpus cavernosum.

Xu L, Xue B, Shan Y, Chen D, Gao J, Yang D, Sun C, Cui Y - Exp Ther Med (2014)

Bottom Line: A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus.The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05).In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum.

View Article: PubMed Central - PubMed

Affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.

ABSTRACT
The aim of the present in vivo study was to determine the presence of muscle-derived stem cells (MDSCs) in the corpus cavernosum of rats. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were performed to detect the expression of the stem cell markers stem cell antigen-1 (Sca-1), Oct4 and Desmin in Sprague-Dawley rats aged 2, 5 and 20 months. Sca-1 was mainly expressed in the blood vessels and cavernous sinus and staining revealed that Sca-1 was predominantly expressed in the cytoplasm. Desmin was primarily expressed in muscular tissues and staining demonstrated that it was mainly expressed in the cytoplasm, however, Desmin was also partially expressed in the nuclei. A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus. It was found that the expression of the markers was negatively correlated with the age of the rats (P<0.05). The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05). In addition, the correlation analysis indicated that the expression of Sca-1 and Desmin were negatively correlated with the age of the rats (r=--0.929; P<0.05). In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum. MDSCs may be a potential therapeutic treatment for organic erectile dysfunction.

No MeSH data available.


Related in: MedlinePlus

Differences in structural density of the corpus cavernosum and the distribution of blood vessels in Sprague-Dawley rats aged (A) two (young group), (B) five (middle-aged group) and (C) 20 months (old group). The cells were stained with hematoxylin and eosin (magnification, ×400).
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f1-etm-08-01-0274: Differences in structural density of the corpus cavernosum and the distribution of blood vessels in Sprague-Dawley rats aged (A) two (young group), (B) five (middle-aged group) and (C) 20 months (old group). The cells were stained with hematoxylin and eosin (magnification, ×400).

Mentions: The corpus cavernosum of the rats gradually changed from tightly organized structures to loosely organized structures as the age of the rats increased. Similarly, the blood vessels were found to be abundant, slightly decreased and significantly decreased in the young, middle-aged and old rats, respectively (Fig. 1).


In vivo determination of muscle-derived stem cells in the rat corpus cavernosum.

Xu L, Xue B, Shan Y, Chen D, Gao J, Yang D, Sun C, Cui Y - Exp Ther Med (2014)

Differences in structural density of the corpus cavernosum and the distribution of blood vessels in Sprague-Dawley rats aged (A) two (young group), (B) five (middle-aged group) and (C) 20 months (old group). The cells were stained with hematoxylin and eosin (magnification, ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061231&req=5

f1-etm-08-01-0274: Differences in structural density of the corpus cavernosum and the distribution of blood vessels in Sprague-Dawley rats aged (A) two (young group), (B) five (middle-aged group) and (C) 20 months (old group). The cells were stained with hematoxylin and eosin (magnification, ×400).
Mentions: The corpus cavernosum of the rats gradually changed from tightly organized structures to loosely organized structures as the age of the rats increased. Similarly, the blood vessels were found to be abundant, slightly decreased and significantly decreased in the young, middle-aged and old rats, respectively (Fig. 1).

Bottom Line: A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus.The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05).In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum.

View Article: PubMed Central - PubMed

Affiliation: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China.

ABSTRACT
The aim of the present in vivo study was to determine the presence of muscle-derived stem cells (MDSCs) in the corpus cavernosum of rats. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were performed to detect the expression of the stem cell markers stem cell antigen-1 (Sca-1), Oct4 and Desmin in Sprague-Dawley rats aged 2, 5 and 20 months. Sca-1 was mainly expressed in the blood vessels and cavernous sinus and staining revealed that Sca-1 was predominantly expressed in the cytoplasm. Desmin was primarily expressed in muscular tissues and staining demonstrated that it was mainly expressed in the cytoplasm, however, Desmin was also partially expressed in the nuclei. A small number of double positive cells, expressing Sca-1 and Desmin, were also detected near the cavernous sinus. It was found that the expression of the markers was negatively correlated with the age of the rats (P<0.05). The results from the RT-PCR demonstrated that the expression levels of Sca-1 and Desmin significantly decreased with age (P<0.05). In addition, the correlation analysis indicated that the expression of Sca-1 and Desmin were negatively correlated with the age of the rats (r=--0.929; P<0.05). In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum. MDSCs may be a potential therapeutic treatment for organic erectile dysfunction.

No MeSH data available.


Related in: MedlinePlus