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Effect of Yangyinqingfei decoction on radiation-induced lung injury via downregulation of MMP12 and TIMP-1 expression.

Li H, Wu H, Gao Y, Cai S - Exp Ther Med (2014)

Bottom Line: The effect of Yangyinqingfei decoction was assessed one, two and four weeks post-irradiation according to the pathological changes and the right lung index (wet weight of right lung/body weight ×100%).Pretreatment with Yangyinqingfei resulted in a significant dose-dependent resistance to radiation-induced body weight loss.The expression of MMP-12 and TIMP-1 increased following irradiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, South Building, Special Inpatient Unit, Chinese PLA General Hospital, Beijing 100853, P.R. China.

ABSTRACT
The aim of this study was to evaluate the effect and underlying mechanism of Yangyinqingfei decoction on radiation-induced lung injury in rats. Wistar rats (n=75) were randomly divided into five experimental groups (A-E). Rats in two of the groups were administered saline solution, whereas rats in the remaining three groups were administered different doses of Yangyinqingfei decoction. After one week, the rats were irradiated with a single dose of 25 Gy to their right hemi-thoraxes by a (60)Co γ-ray, with the exception of the control group, which underwent sham irradiation. The effect of Yangyinqingfei decoction was assessed one, two and four weeks post-irradiation according to the pathological changes and the right lung index (wet weight of right lung/body weight ×100%). Expression levels of matrix metalloproteinase-12 (MMP-12) and tissue inhibitors of metalloproteinases-1 (TIMP-1) in lung tissue were determined using the reverse transcription-polymerase chain reaction and western blot analysis. Pretreatment with Yangyinqingfei resulted in a significant dose-dependent resistance to radiation-induced body weight loss. The expression of MMP-12 and TIMP-1 increased following irradiation. However, the levels of MMP-12 and TIMP-1 in groups receiving Yangyinqingfei were lower four weeks after irradiation compared with those in rats administered saline. Cumulatively, these results suggest that Yangyinqingfei has a protective effect on radiation-induced lung injury in rats, possibly by downregulating MMP-12 and TIMP-1 expression.

No MeSH data available.


Related in: MedlinePlus

Effect of Yangyinqingfei decoction on MMP-12 and TIMP-1 expression. At different time-points following irradiation, total RNA was extracted from lung tissues and reverse transcription-polymerase chain reaction and western blot analysis were performed. (A) mRNA expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation; (B) protein expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation. β-actin was used as an internal control. a, Control group; b, model group (irradiated rats without drug administration); c, low-dose treatment group (2 g/kg/day); d, intermediate-dose treatment group (6 g/kg/day); e, high-dose treatment group (18 g/kg/day); MMP-12, matrix metalloproteinase-12; TIMP-1, tissue inhibitors of matrix metalloproteinase-1; p.i., post-irradiation.
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f2-etm-08-01-0009: Effect of Yangyinqingfei decoction on MMP-12 and TIMP-1 expression. At different time-points following irradiation, total RNA was extracted from lung tissues and reverse transcription-polymerase chain reaction and western blot analysis were performed. (A) mRNA expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation; (B) protein expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation. β-actin was used as an internal control. a, Control group; b, model group (irradiated rats without drug administration); c, low-dose treatment group (2 g/kg/day); d, intermediate-dose treatment group (6 g/kg/day); e, high-dose treatment group (18 g/kg/day); MMP-12, matrix metalloproteinase-12; TIMP-1, tissue inhibitors of matrix metalloproteinase-1; p.i., post-irradiation.

Mentions: To investigate the effects of Yangyinqingfei decoction on mRNA and protein expression of MMP-12 and TIMP-1 in rats with radiation-induced lung injury, RT-PCR and western blot analysis were performed, respectively. As shown in Fig. 2A, the level of MMP-12 mRNA in lung tissues increased following irradiation and peaked at two weeks post-irradiation; however, at four weeks post-irradiation the level of mRNA was reduced markedly. By contrast, the level of TIMP-1 mRNA in lung tissues increased following irradiation and was highest four weeks post-irradiation. In comparison with group B, the levels of MMP-12 and TIMP-1 mRNA in groups C-E were reduced. These results indicate that Yangyinqingfei decoction had an inhibitory effect on mRNA expression of MMP-12 and TIMP-1, particularly at the higher dose.


Effect of Yangyinqingfei decoction on radiation-induced lung injury via downregulation of MMP12 and TIMP-1 expression.

Li H, Wu H, Gao Y, Cai S - Exp Ther Med (2014)

Effect of Yangyinqingfei decoction on MMP-12 and TIMP-1 expression. At different time-points following irradiation, total RNA was extracted from lung tissues and reverse transcription-polymerase chain reaction and western blot analysis were performed. (A) mRNA expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation; (B) protein expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation. β-actin was used as an internal control. a, Control group; b, model group (irradiated rats without drug administration); c, low-dose treatment group (2 g/kg/day); d, intermediate-dose treatment group (6 g/kg/day); e, high-dose treatment group (18 g/kg/day); MMP-12, matrix metalloproteinase-12; TIMP-1, tissue inhibitors of matrix metalloproteinase-1; p.i., post-irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061229&req=5

f2-etm-08-01-0009: Effect of Yangyinqingfei decoction on MMP-12 and TIMP-1 expression. At different time-points following irradiation, total RNA was extracted from lung tissues and reverse transcription-polymerase chain reaction and western blot analysis were performed. (A) mRNA expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation; (B) protein expression of MMP-12 and TIMP-1 one, two and four weeks after irradiation. β-actin was used as an internal control. a, Control group; b, model group (irradiated rats without drug administration); c, low-dose treatment group (2 g/kg/day); d, intermediate-dose treatment group (6 g/kg/day); e, high-dose treatment group (18 g/kg/day); MMP-12, matrix metalloproteinase-12; TIMP-1, tissue inhibitors of matrix metalloproteinase-1; p.i., post-irradiation.
Mentions: To investigate the effects of Yangyinqingfei decoction on mRNA and protein expression of MMP-12 and TIMP-1 in rats with radiation-induced lung injury, RT-PCR and western blot analysis were performed, respectively. As shown in Fig. 2A, the level of MMP-12 mRNA in lung tissues increased following irradiation and peaked at two weeks post-irradiation; however, at four weeks post-irradiation the level of mRNA was reduced markedly. By contrast, the level of TIMP-1 mRNA in lung tissues increased following irradiation and was highest four weeks post-irradiation. In comparison with group B, the levels of MMP-12 and TIMP-1 mRNA in groups C-E were reduced. These results indicate that Yangyinqingfei decoction had an inhibitory effect on mRNA expression of MMP-12 and TIMP-1, particularly at the higher dose.

Bottom Line: The effect of Yangyinqingfei decoction was assessed one, two and four weeks post-irradiation according to the pathological changes and the right lung index (wet weight of right lung/body weight ×100%).Pretreatment with Yangyinqingfei resulted in a significant dose-dependent resistance to radiation-induced body weight loss.The expression of MMP-12 and TIMP-1 increased following irradiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, South Building, Special Inpatient Unit, Chinese PLA General Hospital, Beijing 100853, P.R. China.

ABSTRACT
The aim of this study was to evaluate the effect and underlying mechanism of Yangyinqingfei decoction on radiation-induced lung injury in rats. Wistar rats (n=75) were randomly divided into five experimental groups (A-E). Rats in two of the groups were administered saline solution, whereas rats in the remaining three groups were administered different doses of Yangyinqingfei decoction. After one week, the rats were irradiated with a single dose of 25 Gy to their right hemi-thoraxes by a (60)Co γ-ray, with the exception of the control group, which underwent sham irradiation. The effect of Yangyinqingfei decoction was assessed one, two and four weeks post-irradiation according to the pathological changes and the right lung index (wet weight of right lung/body weight ×100%). Expression levels of matrix metalloproteinase-12 (MMP-12) and tissue inhibitors of metalloproteinases-1 (TIMP-1) in lung tissue were determined using the reverse transcription-polymerase chain reaction and western blot analysis. Pretreatment with Yangyinqingfei resulted in a significant dose-dependent resistance to radiation-induced body weight loss. The expression of MMP-12 and TIMP-1 increased following irradiation. However, the levels of MMP-12 and TIMP-1 in groups receiving Yangyinqingfei were lower four weeks after irradiation compared with those in rats administered saline. Cumulatively, these results suggest that Yangyinqingfei has a protective effect on radiation-induced lung injury in rats, possibly by downregulating MMP-12 and TIMP-1 expression.

No MeSH data available.


Related in: MedlinePlus