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Expression of hypoxia-inducible factor-1α, endothelin-1 and adrenomedullin in newborn rats with hypoxia-induced pulmonary hypertension.

Wang L, Zhou Y, Li M, Zhu Y - Exp Ther Med (2014)

Bottom Line: Hypoxia-inducible factor (HIF)-1α is associated with hypoxia-induced pulmonary hypertension (HPH) in adults.In the present study, the expression levels of HIF-1α, endothelin (ET)-1 and adrenomedullin (ADM) were analyzed during HPH in neonates.At later stages, the mPAP increases, vascular remodeling occurs and HIF-1α, ET-1 and ADM expression levels restore to normal levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Neonatology, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.

ABSTRACT
Hypoxia-inducible factor (HIF)-1α is associated with hypoxia-induced pulmonary hypertension (HPH) in adults. In the present study, the expression levels of HIF-1α, endothelin (ET)-1 and adrenomedullin (ADM) were analyzed during HPH in neonates. In total, 96 newborn rats were subjected to hypoxia or normoxia for 3, 5, 7, 10, 14 or 21 days (n=8 per subgroup). HIF-1α, ET-1 and ADM expression levels were measured by quantitative polymerase chain reaction. In addition, the intima-media thickness/external diameter ratio (MT%) and medial wall cross-sectional area/vessel total cross-sectional area ratio (MA%) were calculated to evaluate pulmonary vascular remodeling. The mean pulmonary arterial pressure (mPAP) increased with exposure to hypoxia. Furthermore, the expression levels of HIF-1α, ET-1 and ADM in the lungs were shown to increase after three and five days of hypoxia, while the MT% and MA% increased after seven days of hypoxia, as compared with the controls (P<0.05). Therefore, the expression of HIF-1α, ET-1 and ADM is upregulated in the lungs of newborn rats during early HPH. At later stages, the mPAP increases, vascular remodeling occurs and HIF-1α, ET-1 and ADM expression levels restore to normal levels.

No MeSH data available.


Related in: MedlinePlus

mRNA expression levels of (A) HIF-1α, (B) ET-1 and (C) ADM in the lung tissues from the various subgroups of newborn rats subjected to hypoxia or normoxia (n=8 per subgroup). Values are expressed as the mean ± standard deviation. *P<0.01, vs. respective control subgroup. HIF, hypoxia-inducible factor; ET, endothelin, ADM, adrenomedullin.
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f1-etm-08-01-0335: mRNA expression levels of (A) HIF-1α, (B) ET-1 and (C) ADM in the lung tissues from the various subgroups of newborn rats subjected to hypoxia or normoxia (n=8 per subgroup). Values are expressed as the mean ± standard deviation. *P<0.01, vs. respective control subgroup. HIF, hypoxia-inducible factor; ET, endothelin, ADM, adrenomedullin.

Mentions: The mRNA expression levels of HIF-1α, ET-1 and ADM in the lungs of the neonatal rats in the hypoxic group were higher at day three and five when compared with the respective control subgroups (P<0.05; Fig. 1). However, at the later time points, the mRNA expression levels of HIF-1α, ET-1 and ADM were not significantly different between the hypoxic and control groups.


Expression of hypoxia-inducible factor-1α, endothelin-1 and adrenomedullin in newborn rats with hypoxia-induced pulmonary hypertension.

Wang L, Zhou Y, Li M, Zhu Y - Exp Ther Med (2014)

mRNA expression levels of (A) HIF-1α, (B) ET-1 and (C) ADM in the lung tissues from the various subgroups of newborn rats subjected to hypoxia or normoxia (n=8 per subgroup). Values are expressed as the mean ± standard deviation. *P<0.01, vs. respective control subgroup. HIF, hypoxia-inducible factor; ET, endothelin, ADM, adrenomedullin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061228&req=5

f1-etm-08-01-0335: mRNA expression levels of (A) HIF-1α, (B) ET-1 and (C) ADM in the lung tissues from the various subgroups of newborn rats subjected to hypoxia or normoxia (n=8 per subgroup). Values are expressed as the mean ± standard deviation. *P<0.01, vs. respective control subgroup. HIF, hypoxia-inducible factor; ET, endothelin, ADM, adrenomedullin.
Mentions: The mRNA expression levels of HIF-1α, ET-1 and ADM in the lungs of the neonatal rats in the hypoxic group were higher at day three and five when compared with the respective control subgroups (P<0.05; Fig. 1). However, at the later time points, the mRNA expression levels of HIF-1α, ET-1 and ADM were not significantly different between the hypoxic and control groups.

Bottom Line: Hypoxia-inducible factor (HIF)-1α is associated with hypoxia-induced pulmonary hypertension (HPH) in adults.In the present study, the expression levels of HIF-1α, endothelin (ET)-1 and adrenomedullin (ADM) were analyzed during HPH in neonates.At later stages, the mPAP increases, vascular remodeling occurs and HIF-1α, ET-1 and ADM expression levels restore to normal levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Neonatology, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang 830011, P.R. China.

ABSTRACT
Hypoxia-inducible factor (HIF)-1α is associated with hypoxia-induced pulmonary hypertension (HPH) in adults. In the present study, the expression levels of HIF-1α, endothelin (ET)-1 and adrenomedullin (ADM) were analyzed during HPH in neonates. In total, 96 newborn rats were subjected to hypoxia or normoxia for 3, 5, 7, 10, 14 or 21 days (n=8 per subgroup). HIF-1α, ET-1 and ADM expression levels were measured by quantitative polymerase chain reaction. In addition, the intima-media thickness/external diameter ratio (MT%) and medial wall cross-sectional area/vessel total cross-sectional area ratio (MA%) were calculated to evaluate pulmonary vascular remodeling. The mean pulmonary arterial pressure (mPAP) increased with exposure to hypoxia. Furthermore, the expression levels of HIF-1α, ET-1 and ADM in the lungs were shown to increase after three and five days of hypoxia, while the MT% and MA% increased after seven days of hypoxia, as compared with the controls (P<0.05). Therefore, the expression of HIF-1α, ET-1 and ADM is upregulated in the lungs of newborn rats during early HPH. At later stages, the mPAP increases, vascular remodeling occurs and HIF-1α, ET-1 and ADM expression levels restore to normal levels.

No MeSH data available.


Related in: MedlinePlus