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MicroRNA-203 inhibits malignant melanoma cell migration by targeting versican.

Bu P, Yang P - Exp Ther Med (2014)

Bottom Line: In the present study, the expression of miR-203 was shown to be significantly downregulated in MM tissues when compared with normal adjacent tissues.These observations indicated that versican functions as a downstream effector in miR-203-mediated MM cell migration.Therefore, the results demonstrated that miR-203 exhibited an inhibitory effect on MM cell migration via directly targeting versican, thus, may become an effective inhibitor for MM metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Burns, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.

ABSTRACT
MicroRNA (miR)-203 has been demonstrated to function as a suppressor in tumorigenesis. Recently, miR-203 was reported to play a role in malignant melanoma (MM); however, the detailed function of miR-203 in MM remains unclear. In the present study, the expression of miR-203 was shown to be significantly downregulated in MM tissues when compared with normal adjacent tissues. Based on a bioinformatic prediction, versican was further identified as a novel target of miR-203, and the expression of versican was markedly increased in MM tissues. Inhibition of miR-203 increased the protein expression of versican, while upregulation of miR-203 inhibited the protein expression of versican in MM A375 cells. In addition, the upregulation of versican significantly promoted A375 cell migration; however, upregulation of miR-203 suppressed A375 cell migration. The present study further investigated whether miR-203 was involved in versican-mediated A375 cell migration, and the results indicated that upregulation of miR-203 significantly inhibited A375 cell migration, which was impaired by overexpression of versican. These observations indicated that versican functions as a downstream effector in miR-203-mediated MM cell migration. Therefore, the results demonstrated that miR-203 exhibited an inhibitory effect on MM cell migration via directly targeting versican, thus, may become an effective inhibitor for MM metastasis.

No MeSH data available.


Related in: MedlinePlus

Effects of versican on MM A375 cell migration. (A) Western blot analysis was performed to determine the protein expression levels of versican in A375 cells transfected with versican plasmid or versican siRNA. (B) Cell migration analysis was performed using A375 cells transfected with versican plasmid or versican siRNA. **P<0.01 vs. control. MM, malignant melanoma.
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f5-etm-08-01-0309: Effects of versican on MM A375 cell migration. (A) Western blot analysis was performed to determine the protein expression levels of versican in A375 cells transfected with versican plasmid or versican siRNA. (B) Cell migration analysis was performed using A375 cells transfected with versican plasmid or versican siRNA. **P<0.01 vs. control. MM, malignant melanoma.

Mentions: Since versican was shown to play a role in the regulation of cell migration (12), the effect of versican on MM A375 cell migration was further investigated. Following transfection of MM A375 cells with the versican plasmid and versican siRNA, the protein expression of versican was analyzed. The results demonstrated that the transfection efficiency was satisfactory (Fig. 5A). It was further demonstrated that overexpression of versican significantly promoted MM A375 cell migration, while siRNA-mediated versican inhibition markedly suppressed A375 cell migration (Fig. 5B). These observations indicate that versican plays a promoting role in MM cell migration.


MicroRNA-203 inhibits malignant melanoma cell migration by targeting versican.

Bu P, Yang P - Exp Ther Med (2014)

Effects of versican on MM A375 cell migration. (A) Western blot analysis was performed to determine the protein expression levels of versican in A375 cells transfected with versican plasmid or versican siRNA. (B) Cell migration analysis was performed using A375 cells transfected with versican plasmid or versican siRNA. **P<0.01 vs. control. MM, malignant melanoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061213&req=5

f5-etm-08-01-0309: Effects of versican on MM A375 cell migration. (A) Western blot analysis was performed to determine the protein expression levels of versican in A375 cells transfected with versican plasmid or versican siRNA. (B) Cell migration analysis was performed using A375 cells transfected with versican plasmid or versican siRNA. **P<0.01 vs. control. MM, malignant melanoma.
Mentions: Since versican was shown to play a role in the regulation of cell migration (12), the effect of versican on MM A375 cell migration was further investigated. Following transfection of MM A375 cells with the versican plasmid and versican siRNA, the protein expression of versican was analyzed. The results demonstrated that the transfection efficiency was satisfactory (Fig. 5A). It was further demonstrated that overexpression of versican significantly promoted MM A375 cell migration, while siRNA-mediated versican inhibition markedly suppressed A375 cell migration (Fig. 5B). These observations indicate that versican plays a promoting role in MM cell migration.

Bottom Line: In the present study, the expression of miR-203 was shown to be significantly downregulated in MM tissues when compared with normal adjacent tissues.These observations indicated that versican functions as a downstream effector in miR-203-mediated MM cell migration.Therefore, the results demonstrated that miR-203 exhibited an inhibitory effect on MM cell migration via directly targeting versican, thus, may become an effective inhibitor for MM metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Burns, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China.

ABSTRACT
MicroRNA (miR)-203 has been demonstrated to function as a suppressor in tumorigenesis. Recently, miR-203 was reported to play a role in malignant melanoma (MM); however, the detailed function of miR-203 in MM remains unclear. In the present study, the expression of miR-203 was shown to be significantly downregulated in MM tissues when compared with normal adjacent tissues. Based on a bioinformatic prediction, versican was further identified as a novel target of miR-203, and the expression of versican was markedly increased in MM tissues. Inhibition of miR-203 increased the protein expression of versican, while upregulation of miR-203 inhibited the protein expression of versican in MM A375 cells. In addition, the upregulation of versican significantly promoted A375 cell migration; however, upregulation of miR-203 suppressed A375 cell migration. The present study further investigated whether miR-203 was involved in versican-mediated A375 cell migration, and the results indicated that upregulation of miR-203 significantly inhibited A375 cell migration, which was impaired by overexpression of versican. These observations indicated that versican functions as a downstream effector in miR-203-mediated MM cell migration. Therefore, the results demonstrated that miR-203 exhibited an inhibitory effect on MM cell migration via directly targeting versican, thus, may become an effective inhibitor for MM metastasis.

No MeSH data available.


Related in: MedlinePlus