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Monoamine neurotransmitters and fibroblast growth factor-2 in the brains of rats with post-stroke depression.

Ji XW, Wu CL, Wang XC, Liu J, Bi JZ, Wang DY - Exp Ther Med (2014)

Bottom Line: High-performance liquid chromatography was used to detect the levels of 5-HT, DA and NE, while western blotting was used to detect the FGF-2 protein expression levels in the frontal lobe and hippocampus.In addition, protein expression levels of FGF-2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF-2 in the hippocampus did not exhibit a statistically significant difference (P>0.05).Therefore, reduced levels of monoamine neurotransmitters and FGF-2 expression in the brains of rats with PSD are associated with the incidence of PSD.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China.

ABSTRACT
The aim of the present study was to investigate the changes in the levels of serotonin (5-HT), dopamine (DA), norepinephrine (NE) and fibroblast growth factor-2 (FGF-2) in the brains of rats with post-stroke depression (PSD). A rat model of stroke was established by middle cerebral artery occlusion and the rats were randomly divided into two groups: Control and modification groups. The rats in the modification group had PSD, while the rats in the control group had experienced a stroke only. The PSD model was established by applying chronic mild stress to the individually housed rats. High-performance liquid chromatography was used to detect the levels of 5-HT, DA and NE, while western blotting was used to detect the FGF-2 protein expression levels in the frontal lobe and hippocampus. Quantitative polymerase chain reaction was also used to determine the mRNA expression levels of FGF-2 in the frontal lobes of the two groups. The levels of 5-HT, DA and NE in the frontal lobe and hippocampus of the rats in the PSD group were significantly lower than the levels observed in the rats in the stroke group (P<0.01). In addition, protein expression levels of FGF-2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF-2 in the hippocampus did not exhibit a statistically significant difference (P>0.05). The mRNA expression levels of FGF-2 in the frontal lobe of the rats in the modification group were significantly lower than the levels in the control group rats (P<0.01). Therefore, reduced levels of monoamine neurotransmitters and FGF-2 expression in the brains of rats with PSD are associated with the incidence of PSD.

No MeSH data available.


Related in: MedlinePlus

mRNA expression levels of FGF-2 in the frontal lobe tissue. #P<0.01, PSD vs. stroke. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression.
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f3-etm-08-01-0159: mRNA expression levels of FGF-2 in the frontal lobe tissue. #P<0.01, PSD vs. stroke. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression.

Mentions: Compared with the stroke group, the mRNA expression levels of FGF-2 in the frontal cortex tissue of the PSD rats were significantly decreased (P<0.01; Student’s t-test; Fig. 3). This marked reduction in the frontal lobe of the rats indicated that the decreased FGF-2 protein expression levels, as observed in the previous experiment, were caused by the downregulation of FGF-2 mRNA expression.


Monoamine neurotransmitters and fibroblast growth factor-2 in the brains of rats with post-stroke depression.

Ji XW, Wu CL, Wang XC, Liu J, Bi JZ, Wang DY - Exp Ther Med (2014)

mRNA expression levels of FGF-2 in the frontal lobe tissue. #P<0.01, PSD vs. stroke. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061212&req=5

f3-etm-08-01-0159: mRNA expression levels of FGF-2 in the frontal lobe tissue. #P<0.01, PSD vs. stroke. FGF-2, fibroblast growth factor-2; PSD, post-stroke depression.
Mentions: Compared with the stroke group, the mRNA expression levels of FGF-2 in the frontal cortex tissue of the PSD rats were significantly decreased (P<0.01; Student’s t-test; Fig. 3). This marked reduction in the frontal lobe of the rats indicated that the decreased FGF-2 protein expression levels, as observed in the previous experiment, were caused by the downregulation of FGF-2 mRNA expression.

Bottom Line: High-performance liquid chromatography was used to detect the levels of 5-HT, DA and NE, while western blotting was used to detect the FGF-2 protein expression levels in the frontal lobe and hippocampus.In addition, protein expression levels of FGF-2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF-2 in the hippocampus did not exhibit a statistically significant difference (P>0.05).Therefore, reduced levels of monoamine neurotransmitters and FGF-2 expression in the brains of rats with PSD are associated with the incidence of PSD.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China.

ABSTRACT
The aim of the present study was to investigate the changes in the levels of serotonin (5-HT), dopamine (DA), norepinephrine (NE) and fibroblast growth factor-2 (FGF-2) in the brains of rats with post-stroke depression (PSD). A rat model of stroke was established by middle cerebral artery occlusion and the rats were randomly divided into two groups: Control and modification groups. The rats in the modification group had PSD, while the rats in the control group had experienced a stroke only. The PSD model was established by applying chronic mild stress to the individually housed rats. High-performance liquid chromatography was used to detect the levels of 5-HT, DA and NE, while western blotting was used to detect the FGF-2 protein expression levels in the frontal lobe and hippocampus. Quantitative polymerase chain reaction was also used to determine the mRNA expression levels of FGF-2 in the frontal lobes of the two groups. The levels of 5-HT, DA and NE in the frontal lobe and hippocampus of the rats in the PSD group were significantly lower than the levels observed in the rats in the stroke group (P<0.01). In addition, protein expression levels of FGF-2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF-2 in the hippocampus did not exhibit a statistically significant difference (P>0.05). The mRNA expression levels of FGF-2 in the frontal lobe of the rats in the modification group were significantly lower than the levels in the control group rats (P<0.01). Therefore, reduced levels of monoamine neurotransmitters and FGF-2 expression in the brains of rats with PSD are associated with the incidence of PSD.

No MeSH data available.


Related in: MedlinePlus