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Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yin Y, Wan J, Li P, Jia Y, Sun R, Pan G, Wan G - Exp Ther Med (2014)

Bottom Line: In addition, XMJ treatment increased cell activity and decreased monolayer permeability.The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration.Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

ABSTRACT
The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti-inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2-induced injury of HUVECs, which may be associated with the NO-cyclic guanosine monophosphate signaling pathway.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence analysis was used to detect the levels of eNOS in HUVECs (magnification, ×400) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; eNOS, endothelial nitric oxide synthase.
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f8-etm-08-01-0038: Immunofluorescence analysis was used to detect the levels of eNOS in HUVECs (magnification, ×400) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; eNOS, endothelial nitric oxide synthase.

Mentions: Confocal fluorescence tomography was performed on immunofluorescence cells with a laser scanning confocal microscope. The 32 facets of each cell were scanned and the fluorescence intensity within the cells was detected using fluorescence quantitative analysis software. The eNOS protein was predominantly expressed in the cytoplasm of the HUVECs. The positive signals presented yellowish-green spotlight, with diffused distribution. The fluorescence intensity values of the eNOS protein were 178.33±11.26 in the high-dose XMJ group and 65.27±4.66 in the model group, which exhibited a statistically significant difference (P<0.05). Lovastatin and zhibituo significantly increased (P<0.05) the fluorescence intensity of the eNOS protein in the HUVECs induced by H2O2. However, the fluorescence intensity of the eNOS protein in the high-dose XMJ group was greater than those in the lovastatin and zhibituo groups (P<0.05) The fluorescence intensities of the eNOS protein in the HUVECs treated with low- and middle-dose XMJ were significantly weaker than that of high-dose XMJ (P<0.05), indicating XMJ dose dependence (Fig. 8).


Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yin Y, Wan J, Li P, Jia Y, Sun R, Pan G, Wan G - Exp Ther Med (2014)

Immunofluorescence analysis was used to detect the levels of eNOS in HUVECs (magnification, ×400) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; eNOS, endothelial nitric oxide synthase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061210&req=5

f8-etm-08-01-0038: Immunofluorescence analysis was used to detect the levels of eNOS in HUVECs (magnification, ×400) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; eNOS, endothelial nitric oxide synthase.
Mentions: Confocal fluorescence tomography was performed on immunofluorescence cells with a laser scanning confocal microscope. The 32 facets of each cell were scanned and the fluorescence intensity within the cells was detected using fluorescence quantitative analysis software. The eNOS protein was predominantly expressed in the cytoplasm of the HUVECs. The positive signals presented yellowish-green spotlight, with diffused distribution. The fluorescence intensity values of the eNOS protein were 178.33±11.26 in the high-dose XMJ group and 65.27±4.66 in the model group, which exhibited a statistically significant difference (P<0.05). Lovastatin and zhibituo significantly increased (P<0.05) the fluorescence intensity of the eNOS protein in the HUVECs induced by H2O2. However, the fluorescence intensity of the eNOS protein in the high-dose XMJ group was greater than those in the lovastatin and zhibituo groups (P<0.05) The fluorescence intensities of the eNOS protein in the HUVECs treated with low- and middle-dose XMJ were significantly weaker than that of high-dose XMJ (P<0.05), indicating XMJ dose dependence (Fig. 8).

Bottom Line: In addition, XMJ treatment increased cell activity and decreased monolayer permeability.The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration.Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

ABSTRACT
The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti-inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2-induced injury of HUVECs, which may be associated with the NO-cyclic guanosine monophosphate signaling pathway.

No MeSH data available.


Related in: MedlinePlus