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Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yin Y, Wan J, Li P, Jia Y, Sun R, Pan G, Wan G - Exp Ther Med (2014)

Bottom Line: In addition, XMJ treatment increased cell activity and decreased monolayer permeability.The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration.Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

ABSTRACT
The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti-inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2-induced injury of HUVECs, which may be associated with the NO-cyclic guanosine monophosphate signaling pathway.

No MeSH data available.


Related in: MedlinePlus

Effect of XMJ on the concentration of SOD and MDA in the supernatants of HUVECs induced by H2O2. *P<0.05, vs. model group; #P<0.05, vs. high-dose XMJ group; △P<0.05, vs. blank control group. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; SOD, superoxide dismutase; MDA, maldionaldehyde; H2O2, hydrogen peroxide.
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f3-etm-08-01-0038: Effect of XMJ on the concentration of SOD and MDA in the supernatants of HUVECs induced by H2O2. *P<0.05, vs. model group; #P<0.05, vs. high-dose XMJ group; △P<0.05, vs. blank control group. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; SOD, superoxide dismutase; MDA, maldionaldehyde; H2O2, hydrogen peroxide.

Mentions: In the high-dose XMJ group, the concentrations of SOD and MDA were 18.21±1.39 U/ml and 1.37±0.26 nmol/ml, respectively. In the model group, the concentration of SOD was 6.35±0.87 U/ml and the concentration of MDA was 1.84±0.26 nmol/ml. Compared with the model group, statistically significant differences (P<0.05) were observed in the high-dose XMJ group with regard to SOD concentration, which significantly increased, and MDA, which significantly decreased. Lovastatin and zhibituo also exhibited marked suppressive effects on the decrease of supernatant SOD levels and the increase of supernatant MDA levels in the HUVEC injury model induced by H2O2. The results were statistically significant (P<0.05) when compared with the model group, however, when compared with the high-dose XMJ group, the differences in HUVEC supernatant SOD and MDA concentrations in the lovastatin and zhibituo groups exhibited no statistically significant differences (P>0.05). The suppressive effects of low- and middle-dose XMJ on the decrease in supernatant SOD concentration and on the increase in supernatant MDA concentration were significantly weaker than that of high-dose XMJ (P<0.05), thus, the effects were dependent on XMJ dosage (Fig. 3).


Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yin Y, Wan J, Li P, Jia Y, Sun R, Pan G, Wan G - Exp Ther Med (2014)

Effect of XMJ on the concentration of SOD and MDA in the supernatants of HUVECs induced by H2O2. *P<0.05, vs. model group; #P<0.05, vs. high-dose XMJ group; △P<0.05, vs. blank control group. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; SOD, superoxide dismutase; MDA, maldionaldehyde; H2O2, hydrogen peroxide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061210&req=5

f3-etm-08-01-0038: Effect of XMJ on the concentration of SOD and MDA in the supernatants of HUVECs induced by H2O2. *P<0.05, vs. model group; #P<0.05, vs. high-dose XMJ group; △P<0.05, vs. blank control group. XMJ, Xin Mai Jia; HUVECs, human umbilical vein endothelial cells; SOD, superoxide dismutase; MDA, maldionaldehyde; H2O2, hydrogen peroxide.
Mentions: In the high-dose XMJ group, the concentrations of SOD and MDA were 18.21±1.39 U/ml and 1.37±0.26 nmol/ml, respectively. In the model group, the concentration of SOD was 6.35±0.87 U/ml and the concentration of MDA was 1.84±0.26 nmol/ml. Compared with the model group, statistically significant differences (P<0.05) were observed in the high-dose XMJ group with regard to SOD concentration, which significantly increased, and MDA, which significantly decreased. Lovastatin and zhibituo also exhibited marked suppressive effects on the decrease of supernatant SOD levels and the increase of supernatant MDA levels in the HUVEC injury model induced by H2O2. The results were statistically significant (P<0.05) when compared with the model group, however, when compared with the high-dose XMJ group, the differences in HUVEC supernatant SOD and MDA concentrations in the lovastatin and zhibituo groups exhibited no statistically significant differences (P>0.05). The suppressive effects of low- and middle-dose XMJ on the decrease in supernatant SOD concentration and on the increase in supernatant MDA concentration were significantly weaker than that of high-dose XMJ (P<0.05), thus, the effects were dependent on XMJ dosage (Fig. 3).

Bottom Line: In addition, XMJ treatment increased cell activity and decreased monolayer permeability.The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration.Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

ABSTRACT
The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti-inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2-induced injury of HUVECs, which may be associated with the NO-cyclic guanosine monophosphate signaling pathway.

No MeSH data available.


Related in: MedlinePlus