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Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yin Y, Wan J, Li P, Jia Y, Sun R, Pan G, Wan G - Exp Ther Med (2014)

Bottom Line: In addition, XMJ treatment increased cell activity and decreased monolayer permeability.The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration.Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

ABSTRACT
The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti-inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2-induced injury of HUVECs, which may be associated with the NO-cyclic guanosine monophosphate signaling pathway.

No MeSH data available.


Related in: MedlinePlus

Microscopic images showing the protective effect of XMJ on HUVEC injury induced by H2O2 (magnification, ×400; HE stain) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVEC, human umbilical vein endothelial cell; HE, hematoxylin and eosin; H2O2, hydrogen peroxide.
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f1-etm-08-01-0038: Microscopic images showing the protective effect of XMJ on HUVEC injury induced by H2O2 (magnification, ×400; HE stain) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVEC, human umbilical vein endothelial cell; HE, hematoxylin and eosin; H2O2, hydrogen peroxide.

Mentions: HUVECs stained with HE were observed under a microscope (magnification, ×400) and the observations were as follows. HUVEC apoptosis was significantly reduced in the high-dose XMJ group. Cytoplasmic staining was relatively uniform and the metachromatic particles of the nuclei were not marked. The cells were arranged closely and their morphology was normal. Evident differences were identified when comparing these cells with the model group. Higher-dose XMJ demonstrated a significant protective effect on the HUVEC injury induced by H2O2. Lovastatin and zhibituo also exhibited marked protective effects, however, their protective effects with regard to morphology were relatively weaker when compared with the high-dose XMJ group. The protective effects of the low- and middle-dose XMJ groups were significantly weaker than that of the high-dose XMJ group, indicating the dependence of the protective effect on XMJ dosage (Fig. 1).


Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO-cGMP signaling pathway.

Yin Y, Wan J, Li P, Jia Y, Sun R, Pan G, Wan G - Exp Ther Med (2014)

Microscopic images showing the protective effect of XMJ on HUVEC injury induced by H2O2 (magnification, ×400; HE stain) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVEC, human umbilical vein endothelial cell; HE, hematoxylin and eosin; H2O2, hydrogen peroxide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061210&req=5

f1-etm-08-01-0038: Microscopic images showing the protective effect of XMJ on HUVEC injury induced by H2O2 (magnification, ×400; HE stain) in the (A) blank control, (B) XMJ control, (C) model, (D) lovastatin, (E) zhibituo, (F) low-dose XMJ, (G) medium-dose XMJ and (H) high-dose XMJ groups. XMJ, Xin Mai Jia; HUVEC, human umbilical vein endothelial cell; HE, hematoxylin and eosin; H2O2, hydrogen peroxide.
Mentions: HUVECs stained with HE were observed under a microscope (magnification, ×400) and the observations were as follows. HUVEC apoptosis was significantly reduced in the high-dose XMJ group. Cytoplasmic staining was relatively uniform and the metachromatic particles of the nuclei were not marked. The cells were arranged closely and their morphology was normal. Evident differences were identified when comparing these cells with the model group. Higher-dose XMJ demonstrated a significant protective effect on the HUVEC injury induced by H2O2. Lovastatin and zhibituo also exhibited marked protective effects, however, their protective effects with regard to morphology were relatively weaker when compared with the high-dose XMJ group. The protective effects of the low- and middle-dose XMJ groups were significantly weaker than that of the high-dose XMJ group, indicating the dependence of the protective effect on XMJ dosage (Fig. 1).

Bottom Line: In addition, XMJ treatment increased cell activity and decreased monolayer permeability.The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration.Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.

ABSTRACT
The aim of the present study was to evaluate the protective effect of the ultrafiltration extract of Xin Mai Jia (XMJ) on a human umbilical vein endothelial cell (HUVEC) injury model induced by hydrogen peroxide (H2O2), by providing experimental data to investigate the mechanism and efficacy underlying the therapeutic effects on atherosclerosis. HUVECs were first injured by H2O2 and then varying final concentrations of the Chinese herb extract were added. Effects of the XMJ extract on morphology, activity, monolayer permeability, biochemical indicators, cytokines, endothelial nitric oxide synthase (eNOS) protein content and eNOS gene expression in the HUVECs were analyzed. H2O2 significantly promoted HUVEC injury. The XMJ ultrafiltration extract significantly improved the morphological changes in the injured HUVECs. In addition, XMJ treatment increased cell activity and decreased monolayer permeability. The expression levels of intracellular adhesion molecule-1, vascular adhesion molecule-1, interleukin-1 and -6 and nuclear factor-κB decreased, while the expression levels of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 increased with XMJ administration. Increased levels of nitric oxide (NO), eNOS protein and eNOS gene expression were also observed. Therefore, the XMJ ultrafiltration extract exhibits marked anti-inflammatory effects and antioxidant abilities. These properties significantly inhibited the H2O2-induced injury of HUVECs, which may be associated with the NO-cyclic guanosine monophosphate signaling pathway.

No MeSH data available.


Related in: MedlinePlus