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Qianliening capsule inhibits benign prostatic hyperplasia angiogenesis via the HIF-1α signaling pathway.

Lin J, Zhou J, Xu W, Hong Z, Peng J - Exp Ther Med (2014)

Bottom Line: However, the mechanisms underlying the anti-BPH effect remain largely unknown.QC was shown to reduce the prostatic index in BPH rats, but without affecting the body weight, demonstrating that QC is effective in the treatment of BPH and without apparent toxicity.In addition, QC treatment significantly reduced the intraprostatic microvessel density, indicating antiangiogenesis activity in vivo.

View Article: PubMed Central - PubMed

Affiliation: Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT
Angiogenesis plays an important role in the progression and development of benign prostatic hyperplasia (BPH), and has become a promising target for BPH treatment. The hypoxia-inducible factor-1α (HIF-1α) signaling pathway promotes the process of angiogenesis, contributing to the growth and progression of a number of hyperplasia diseases, including BPH. Qianliening capsule (QC) is a traditional Chinese formula that has been used clinically in China to treat BPH for a number of years. Recently, QC was demonstrated to inhibit prostatic cell growth and induce apoptosis in vivo and in vitro via regulating the epidermal growth factor/signal transducer and activator of transcription 3 signaling pathway and mitochondrion-dependent apoptosis pathway. However, the mechanisms underlying the anti-BPH effect remain largely unknown. To further elucidate the mechanism of QC activity in BPH treatment, a rat BPH model established by injecting testosterone following castration was established and the effect of QC on prostatic tissue angiogenesis was evaluated, as well as the underlying molecular mechanisms. QC was shown to reduce the prostatic index in BPH rats, but without affecting the body weight, demonstrating that QC is effective in the treatment of BPH and without apparent toxicity. In addition, QC treatment significantly reduced the intraprostatic microvessel density, indicating antiangiogenesis activity in vivo. In addition, treatment with QC inhibited the expression of HIF-1α in BPH rats, as well as the expression of vascular endothelial growth factor and basic fibroblast growth factor. Therefore, for the first time, the present study hypothesized that QC inhibits angiogenesis in prostatic tissue of BPH rats via the inhibition of the HIF-1α signaling pathway, which may be one of the mechanisms in which QC treats BPH.

No MeSH data available.


Related in: MedlinePlus

Inhibition effect of QC on the intraprostatic MVD in BPH rats. CD31 expression was observed using IHC staining (magnification, ×400) and quantification of the IHC assay was represented as the percentage of positively-stained cells. Data are expressed as the mean ± standard deviation (error bars) from 10 individual rats in each group. *P<0.01, vs. control; #P<0.01, vs. model. QC, qianliening capsule; BPH, benign prostatic hyperplasia; IHC, immunohistochemistry; MVD, microvessel density.
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f2-etm-08-01-0118: Inhibition effect of QC on the intraprostatic MVD in BPH rats. CD31 expression was observed using IHC staining (magnification, ×400) and quantification of the IHC assay was represented as the percentage of positively-stained cells. Data are expressed as the mean ± standard deviation (error bars) from 10 individual rats in each group. *P<0.01, vs. control; #P<0.01, vs. model. QC, qianliening capsule; BPH, benign prostatic hyperplasia; IHC, immunohistochemistry; MVD, microvessel density.

Mentions: IHC staining for the EC-specific marker, CD31, was performed to determine the effect of QC on intratumoral microvessel density (MVD), which is considered to be an indicator of angiogenesis. As shown in Fig. 2, the percentage of CD31-positive cells in the model group significantly increased when compared with the control group (P<0.01); however, treatment with QC significantly decreased the number of CD31-positive cells when compared with the model group. These observations indicated that inhibition of BPH tissue angiogenesis by QC may contribute to the inhibition of prostatic cell proliferation.


Qianliening capsule inhibits benign prostatic hyperplasia angiogenesis via the HIF-1α signaling pathway.

Lin J, Zhou J, Xu W, Hong Z, Peng J - Exp Ther Med (2014)

Inhibition effect of QC on the intraprostatic MVD in BPH rats. CD31 expression was observed using IHC staining (magnification, ×400) and quantification of the IHC assay was represented as the percentage of positively-stained cells. Data are expressed as the mean ± standard deviation (error bars) from 10 individual rats in each group. *P<0.01, vs. control; #P<0.01, vs. model. QC, qianliening capsule; BPH, benign prostatic hyperplasia; IHC, immunohistochemistry; MVD, microvessel density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061199&req=5

f2-etm-08-01-0118: Inhibition effect of QC on the intraprostatic MVD in BPH rats. CD31 expression was observed using IHC staining (magnification, ×400) and quantification of the IHC assay was represented as the percentage of positively-stained cells. Data are expressed as the mean ± standard deviation (error bars) from 10 individual rats in each group. *P<0.01, vs. control; #P<0.01, vs. model. QC, qianliening capsule; BPH, benign prostatic hyperplasia; IHC, immunohistochemistry; MVD, microvessel density.
Mentions: IHC staining for the EC-specific marker, CD31, was performed to determine the effect of QC on intratumoral microvessel density (MVD), which is considered to be an indicator of angiogenesis. As shown in Fig. 2, the percentage of CD31-positive cells in the model group significantly increased when compared with the control group (P<0.01); however, treatment with QC significantly decreased the number of CD31-positive cells when compared with the model group. These observations indicated that inhibition of BPH tissue angiogenesis by QC may contribute to the inhibition of prostatic cell proliferation.

Bottom Line: However, the mechanisms underlying the anti-BPH effect remain largely unknown.QC was shown to reduce the prostatic index in BPH rats, but without affecting the body weight, demonstrating that QC is effective in the treatment of BPH and without apparent toxicity.In addition, QC treatment significantly reduced the intraprostatic microvessel density, indicating antiangiogenesis activity in vivo.

View Article: PubMed Central - PubMed

Affiliation: Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT
Angiogenesis plays an important role in the progression and development of benign prostatic hyperplasia (BPH), and has become a promising target for BPH treatment. The hypoxia-inducible factor-1α (HIF-1α) signaling pathway promotes the process of angiogenesis, contributing to the growth and progression of a number of hyperplasia diseases, including BPH. Qianliening capsule (QC) is a traditional Chinese formula that has been used clinically in China to treat BPH for a number of years. Recently, QC was demonstrated to inhibit prostatic cell growth and induce apoptosis in vivo and in vitro via regulating the epidermal growth factor/signal transducer and activator of transcription 3 signaling pathway and mitochondrion-dependent apoptosis pathway. However, the mechanisms underlying the anti-BPH effect remain largely unknown. To further elucidate the mechanism of QC activity in BPH treatment, a rat BPH model established by injecting testosterone following castration was established and the effect of QC on prostatic tissue angiogenesis was evaluated, as well as the underlying molecular mechanisms. QC was shown to reduce the prostatic index in BPH rats, but without affecting the body weight, demonstrating that QC is effective in the treatment of BPH and without apparent toxicity. In addition, QC treatment significantly reduced the intraprostatic microvessel density, indicating antiangiogenesis activity in vivo. In addition, treatment with QC inhibited the expression of HIF-1α in BPH rats, as well as the expression of vascular endothelial growth factor and basic fibroblast growth factor. Therefore, for the first time, the present study hypothesized that QC inhibits angiogenesis in prostatic tissue of BPH rats via the inhibition of the HIF-1α signaling pathway, which may be one of the mechanisms in which QC treats BPH.

No MeSH data available.


Related in: MedlinePlus