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Analysis of differentially expressed genes in cold-exposed mice to investigate the potential causes of cold-induced hypertension.

Tuo B, Li C, Peng L, Ye M, Liu W, Zhong X, Li H - Exp Ther Med (2014)

Bottom Line: Analysis results indicated that the differentially expressed genes with the most significance were associated with adaptive thermogenesis, fatty acid metabolism and energy metabolism.In addition, genes associated with oxidative stress were significantly upregulated, including superoxide dismutase 2 (SOD2) and epoxide hydrolase 2 (EPHX2).Therefore, the results of the present study indicate that tissue hypoxia and increased oxidative stress may play important roles in the process of CIH.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, 451 Hospital of PLA, Xi'an, Shaanxi 710054, P.R. China.

ABSTRACT
Cold exposure is considered to be an important contributing factor to the high morbidity of hypertension. In order to elucidate the cause and mechanism of cold-induced hypertension (CIH), gene expression analysis was performed on microarray data for two groups of cold-exposed mice (4°C for 1 week and 4°C for 5 weeks, three replicates per group) and their respective control groups maintained at 30°C. Analysis results indicated that the differentially expressed genes with the most significance were associated with adaptive thermogenesis, fatty acid metabolism and energy metabolism. The expected marked increase in metabolism during cold exposure caused tissue hypoxia. Genes involved in the hypoxia-inducible factor signaling pathway were activated. In addition, genes associated with oxidative stress were significantly upregulated, including superoxide dismutase 2 (SOD2) and epoxide hydrolase 2 (EPHX2). The majority of genes involved in inflammation-associated pathways were shown to be downregulated in the 4°C 5-week group. Therefore, the results of the present study indicate that tissue hypoxia and increased oxidative stress may play important roles in the process of CIH.

No MeSH data available.


Related in: MedlinePlus

Heatmaps demonstrating the expression levels of blood pressure-associated genes in (A) all mice and the (B) 4°C 1 week and (C) 4°C 5 week groups. Information for each sample corresponding to Table I is shown on the x-axis.
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f2-etm-08-01-0110: Heatmaps demonstrating the expression levels of blood pressure-associated genes in (A) all mice and the (B) 4°C 1 week and (C) 4°C 5 week groups. Information for each sample corresponding to Table I is shown on the x-axis.

Mentions: A total of 52 DEGs associated with blood pressure regulation were selected according to the GO terms and descriptions. Expression patterns were constructed with clustering analysis and are shown in Fig. 2. In total, 18 DEGs were observed that were differentially expressed after 1 and 5 weeks of exposure. In addition, six genes were identified to have a larger difference at the expression level with long term exposure, including superoxide dismutase 2 (SOD2), glucose-6-phosphate dehydrogenase X-linked (G6pdx), WNK lysine deficient protein kinase 1 (Wnk1), peroxisome proliferator activated receptor α (Ppara), cytochrome b-245, α polypeptide (Cyba) and ATPase, Na+/K+ transporting, α 2 polypeptide (Atp1a2). Furthermore, there were a number of 1 week specific DEGs that exhibited a transient expression pattern following 1 week exposure, but returned to the initial values by week 5, including angiotensin II receptor type 1a (Agtr1a) and adrenergic receptor α 1a (Adra1a). There were also specific genes considered to be slow-response genes that were only observed after 5 weeks of cold exposure.


Analysis of differentially expressed genes in cold-exposed mice to investigate the potential causes of cold-induced hypertension.

Tuo B, Li C, Peng L, Ye M, Liu W, Zhong X, Li H - Exp Ther Med (2014)

Heatmaps demonstrating the expression levels of blood pressure-associated genes in (A) all mice and the (B) 4°C 1 week and (C) 4°C 5 week groups. Information for each sample corresponding to Table I is shown on the x-axis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061198&req=5

f2-etm-08-01-0110: Heatmaps demonstrating the expression levels of blood pressure-associated genes in (A) all mice and the (B) 4°C 1 week and (C) 4°C 5 week groups. Information for each sample corresponding to Table I is shown on the x-axis.
Mentions: A total of 52 DEGs associated with blood pressure regulation were selected according to the GO terms and descriptions. Expression patterns were constructed with clustering analysis and are shown in Fig. 2. In total, 18 DEGs were observed that were differentially expressed after 1 and 5 weeks of exposure. In addition, six genes were identified to have a larger difference at the expression level with long term exposure, including superoxide dismutase 2 (SOD2), glucose-6-phosphate dehydrogenase X-linked (G6pdx), WNK lysine deficient protein kinase 1 (Wnk1), peroxisome proliferator activated receptor α (Ppara), cytochrome b-245, α polypeptide (Cyba) and ATPase, Na+/K+ transporting, α 2 polypeptide (Atp1a2). Furthermore, there were a number of 1 week specific DEGs that exhibited a transient expression pattern following 1 week exposure, but returned to the initial values by week 5, including angiotensin II receptor type 1a (Agtr1a) and adrenergic receptor α 1a (Adra1a). There were also specific genes considered to be slow-response genes that were only observed after 5 weeks of cold exposure.

Bottom Line: Analysis results indicated that the differentially expressed genes with the most significance were associated with adaptive thermogenesis, fatty acid metabolism and energy metabolism.In addition, genes associated with oxidative stress were significantly upregulated, including superoxide dismutase 2 (SOD2) and epoxide hydrolase 2 (EPHX2).Therefore, the results of the present study indicate that tissue hypoxia and increased oxidative stress may play important roles in the process of CIH.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, 451 Hospital of PLA, Xi'an, Shaanxi 710054, P.R. China.

ABSTRACT
Cold exposure is considered to be an important contributing factor to the high morbidity of hypertension. In order to elucidate the cause and mechanism of cold-induced hypertension (CIH), gene expression analysis was performed on microarray data for two groups of cold-exposed mice (4°C for 1 week and 4°C for 5 weeks, three replicates per group) and their respective control groups maintained at 30°C. Analysis results indicated that the differentially expressed genes with the most significance were associated with adaptive thermogenesis, fatty acid metabolism and energy metabolism. The expected marked increase in metabolism during cold exposure caused tissue hypoxia. Genes involved in the hypoxia-inducible factor signaling pathway were activated. In addition, genes associated with oxidative stress were significantly upregulated, including superoxide dismutase 2 (SOD2) and epoxide hydrolase 2 (EPHX2). The majority of genes involved in inflammation-associated pathways were shown to be downregulated in the 4°C 5-week group. Therefore, the results of the present study indicate that tissue hypoxia and increased oxidative stress may play important roles in the process of CIH.

No MeSH data available.


Related in: MedlinePlus