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Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice.

Chen S, Zhu K, Wang R, Zhao X - Exp Ther Med (2014)

Bottom Line: A high dose of PLYCSB (50 mg/kg) was found to reduce the levels of the serum proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, as well as increase the levels of IL-4 compared with those in mice treated with a low dose of PLYCSB (25 mg/kg) and control mice.These results suggest that PLYCSB has an inhibitory effect against gastric injury that is comparable to that of the gastric injury drug omeprazole.Therefore, PLYCSB has the potential to be used as a natural therapeutic drug.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.

ABSTRACT
In the present study the preventive effect of polysaccharides from the large yellow croaker swim bladder (PLYCSB) on HCl/ethanol-induced gastric injury in ICR mice was investigated. A high dose of PLYCSB (50 mg/kg) was found to reduce the levels of the serum proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, as well as increase the levels of IL-4 compared with those in mice treated with a low dose of PLYCSB (25 mg/kg) and control mice. The somatostatin and vasoactive intestinal peptide serum levels in PLYCSB-treated mice were higher compared with those in control mice, whilst motilin and substance P serum levels were lower compared with those in control mice. The extent of the gastric injury in the mice treated with PLYCSB was lower compared with that in the control mice; however, the results obtained for mice treated with a high dose of PLYCSB were similar to those for omeprazole-treated mice. In addition, the superoxide dismutase and glutathione peroxidase activities of PLYCSB-treated mice were higher compared with those of the control mice, and similar to those observed in normal and omeprazole-treated mice. Furthermore, PLYCSB-treated mice showed levels of nitric oxide and malondialdehyde that were similar to those in the normal group. Using PCR and western blot analysis, it was demonstrated that PLYCSB significantly inhibited inflammation in the tissues of the HCl/ethanol induced gastric injury mice by downregulating the expression of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α and IL-1β. These results suggest that PLYCSB has an inhibitory effect against gastric injury that is comparable to that of the gastric injury drug omeprazole. Therefore, PLYCSB has the potential to be used as a natural therapeutic drug.

No MeSH data available.


Related in: MedlinePlus

Gastric secretion volume of mice treated with PLYCSB following the induction of gastric injury with HCl/ethanol. Mean values with different letters over the bars are significantly different (P<0.05) according to Duncan’s multiple range test. PLYCSB, polysaccharides of large yellow croaker swim bladder.
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f2-etm-08-01-0316: Gastric secretion volume of mice treated with PLYCSB following the induction of gastric injury with HCl/ethanol. Mean values with different letters over the bars are significantly different (P<0.05) according to Duncan’s multiple range test. PLYCSB, polysaccharides of large yellow croaker swim bladder.

Mentions: The gastric secretion volume of normal mice was the lowest (0.15±0.07 ml) among all the groups (Fig. 2A). The volume was increased in the control mice (0.75±0.11 ml); however, the increase was attenuated following treatment with 25 mg/kg PLYCSB (0.47±0.09 ml), 50 mg/kg PLYCSB (0.31±0.06 ml) and omeprazole (0.24±0.05 ml). The pH values of the gastric juices were 3.68±0.42, 0.98±0.12, 1.74±0.27, 2.51±0.21 and 2.86±0.22 for the normal, control, 25 mg/kg PLYCSB, 50 mg/kg PLYCSB and omeprazole groups, respectively (Fig. 2B). These results demonstrate that there is a reduction in gastric secretion and an increase in gastric pH in mice treated with PLYCSB compared with those in the control mice.


Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice.

Chen S, Zhu K, Wang R, Zhao X - Exp Ther Med (2014)

Gastric secretion volume of mice treated with PLYCSB following the induction of gastric injury with HCl/ethanol. Mean values with different letters over the bars are significantly different (P<0.05) according to Duncan’s multiple range test. PLYCSB, polysaccharides of large yellow croaker swim bladder.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061197&req=5

f2-etm-08-01-0316: Gastric secretion volume of mice treated with PLYCSB following the induction of gastric injury with HCl/ethanol. Mean values with different letters over the bars are significantly different (P<0.05) according to Duncan’s multiple range test. PLYCSB, polysaccharides of large yellow croaker swim bladder.
Mentions: The gastric secretion volume of normal mice was the lowest (0.15±0.07 ml) among all the groups (Fig. 2A). The volume was increased in the control mice (0.75±0.11 ml); however, the increase was attenuated following treatment with 25 mg/kg PLYCSB (0.47±0.09 ml), 50 mg/kg PLYCSB (0.31±0.06 ml) and omeprazole (0.24±0.05 ml). The pH values of the gastric juices were 3.68±0.42, 0.98±0.12, 1.74±0.27, 2.51±0.21 and 2.86±0.22 for the normal, control, 25 mg/kg PLYCSB, 50 mg/kg PLYCSB and omeprazole groups, respectively (Fig. 2B). These results demonstrate that there is a reduction in gastric secretion and an increase in gastric pH in mice treated with PLYCSB compared with those in the control mice.

Bottom Line: A high dose of PLYCSB (50 mg/kg) was found to reduce the levels of the serum proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, as well as increase the levels of IL-4 compared with those in mice treated with a low dose of PLYCSB (25 mg/kg) and control mice.These results suggest that PLYCSB has an inhibitory effect against gastric injury that is comparable to that of the gastric injury drug omeprazole.Therefore, PLYCSB has the potential to be used as a natural therapeutic drug.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.

ABSTRACT
In the present study the preventive effect of polysaccharides from the large yellow croaker swim bladder (PLYCSB) on HCl/ethanol-induced gastric injury in ICR mice was investigated. A high dose of PLYCSB (50 mg/kg) was found to reduce the levels of the serum proinflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, as well as increase the levels of IL-4 compared with those in mice treated with a low dose of PLYCSB (25 mg/kg) and control mice. The somatostatin and vasoactive intestinal peptide serum levels in PLYCSB-treated mice were higher compared with those in control mice, whilst motilin and substance P serum levels were lower compared with those in control mice. The extent of the gastric injury in the mice treated with PLYCSB was lower compared with that in the control mice; however, the results obtained for mice treated with a high dose of PLYCSB were similar to those for omeprazole-treated mice. In addition, the superoxide dismutase and glutathione peroxidase activities of PLYCSB-treated mice were higher compared with those of the control mice, and similar to those observed in normal and omeprazole-treated mice. Furthermore, PLYCSB-treated mice showed levels of nitric oxide and malondialdehyde that were similar to those in the normal group. Using PCR and western blot analysis, it was demonstrated that PLYCSB significantly inhibited inflammation in the tissues of the HCl/ethanol induced gastric injury mice by downregulating the expression of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α and IL-1β. These results suggest that PLYCSB has an inhibitory effect against gastric injury that is comparable to that of the gastric injury drug omeprazole. Therefore, PLYCSB has the potential to be used as a natural therapeutic drug.

No MeSH data available.


Related in: MedlinePlus