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(18)F-FLT PET/CT imaging in a Wister rabbit inflammation model.

Tan Y, Liang J, Liu D, Zhu F, Wang G, Ding X, Han C - Exp Ther Med (2014)

Bottom Line: A total of eight rabbits were implanted with S. aureus in the left tibia to induce an inflammatory process.Calcium sulphate + gentamicin was implanted in the right tibia to induce a physical stimulus without bacterial multiplication.The uptake of (18)F-FLT was significantly higher in the abscess site compared with that in the granuloma, with maximum standardised uptake values of 5.76±0.25 and 1.15±0.32, respectively (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: PET-CT Center, The Affiliated School of Clinical Medicine of Xuzhou Medical College, Xuzhou, Jiangsu 221009, P.R. China.

ABSTRACT
The aim of the present study was to determine the tumour specificity of the newly developed nucleoside metabolic positron emission tomography (PET) tracer, 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT). Using (18)F-FLT PET imaging, DNA synthesis and cell proliferation were detected in Staphylococcus aureus (S. aureus) abscess and calcium sulphate models in Wister rabbits. A total of eight rabbits were implanted with S. aureus in the left tibia to induce an inflammatory process. Calcium sulphate + gentamicin was implanted in the right tibia to induce a physical stimulus without bacterial multiplication. After four weeks, the animals underwent (18)F-FLT PET imaging, bacterial culturing and tissue pathology. The uptake of (18)F-FLT was significantly higher in the abscess site compared with that in the granuloma, with maximum standardised uptake values of 5.76±0.25 and 1.15±0.32, respectively (P<0.01). This indicates that (18)F-FLT is not a specific tumour tracer since active inflammation also results in the uptake of this compound. However, the tumour specificity of this tracer is higher compared with that of (18)F-fluorodeoxyglucose. Therefore, (18)F-FLT may be useful in the differential diagnosis of benign and malignant tumours.

No MeSH data available.


Related in: MedlinePlus

Calcium sulphate and degradation products are surrounded by fibrous tissue. No inflammatory cells, bone necrosis or local abscesses are visible in the medullary cavity. Haematoxylin and eosin staining. Magnification, ×4.
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f1-etm-08-01-0069: Calcium sulphate and degradation products are surrounded by fibrous tissue. No inflammatory cells, bone necrosis or local abscesses are visible in the medullary cavity. Haematoxylin and eosin staining. Magnification, ×4.

Mentions: In group A, calcium sulphate and the degradation products were surrounded by fibrous tissue and there were no inflammatory cells, necrosis of the bone or local abscesses present in the medullary cavity (Fig. 1). In group B, a certain amount of inflammatory cells and S. aureus abscess formation were visible. In addition, a small degree of new bone formation over the scar tissues was observed (Fig. 2).


(18)F-FLT PET/CT imaging in a Wister rabbit inflammation model.

Tan Y, Liang J, Liu D, Zhu F, Wang G, Ding X, Han C - Exp Ther Med (2014)

Calcium sulphate and degradation products are surrounded by fibrous tissue. No inflammatory cells, bone necrosis or local abscesses are visible in the medullary cavity. Haematoxylin and eosin staining. Magnification, ×4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061190&req=5

f1-etm-08-01-0069: Calcium sulphate and degradation products are surrounded by fibrous tissue. No inflammatory cells, bone necrosis or local abscesses are visible in the medullary cavity. Haematoxylin and eosin staining. Magnification, ×4.
Mentions: In group A, calcium sulphate and the degradation products were surrounded by fibrous tissue and there were no inflammatory cells, necrosis of the bone or local abscesses present in the medullary cavity (Fig. 1). In group B, a certain amount of inflammatory cells and S. aureus abscess formation were visible. In addition, a small degree of new bone formation over the scar tissues was observed (Fig. 2).

Bottom Line: A total of eight rabbits were implanted with S. aureus in the left tibia to induce an inflammatory process.Calcium sulphate + gentamicin was implanted in the right tibia to induce a physical stimulus without bacterial multiplication.The uptake of (18)F-FLT was significantly higher in the abscess site compared with that in the granuloma, with maximum standardised uptake values of 5.76±0.25 and 1.15±0.32, respectively (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: PET-CT Center, The Affiliated School of Clinical Medicine of Xuzhou Medical College, Xuzhou, Jiangsu 221009, P.R. China.

ABSTRACT
The aim of the present study was to determine the tumour specificity of the newly developed nucleoside metabolic positron emission tomography (PET) tracer, 3'-deoxy-3'-(18)F-fluorothymidine ((18)F-FLT). Using (18)F-FLT PET imaging, DNA synthesis and cell proliferation were detected in Staphylococcus aureus (S. aureus) abscess and calcium sulphate models in Wister rabbits. A total of eight rabbits were implanted with S. aureus in the left tibia to induce an inflammatory process. Calcium sulphate + gentamicin was implanted in the right tibia to induce a physical stimulus without bacterial multiplication. After four weeks, the animals underwent (18)F-FLT PET imaging, bacterial culturing and tissue pathology. The uptake of (18)F-FLT was significantly higher in the abscess site compared with that in the granuloma, with maximum standardised uptake values of 5.76±0.25 and 1.15±0.32, respectively (P<0.01). This indicates that (18)F-FLT is not a specific tumour tracer since active inflammation also results in the uptake of this compound. However, the tumour specificity of this tracer is higher compared with that of (18)F-fluorodeoxyglucose. Therefore, (18)F-FLT may be useful in the differential diagnosis of benign and malignant tumours.

No MeSH data available.


Related in: MedlinePlus